Open Access

Construction of a 14‑lncRNA risk score system predicting survival of children with acute myelocytic leukemia

  • Authors:
    • Shuli Guo
    • Bo Li
    • Xiaoyan Xu
    • Wanli Wang
    • Songyun Wang
    • Tao Lv
    • Huirui Wang
  • View Affiliations

  • Published online on: June 5, 2020     https://doi.org/10.3892/etm.2020.8846
  • Pages: 1521-1531
  • Copyright: © Guo et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Acute myelocytic leukemia (AML) is a frequent type of acute leukemia. The present study was performed to build a risk score system for the prognostic prediction of AML. AML RNA‑sequencing data from samples from 111 children were downloaded from The Cancer Genome Atlas database. Using the DEseq and edgeR packages, the differentially expressed long non‑coding RNAs (DE‑lncRNAs) between bad and good prognosis groups were identified. A survival package was used to screen prognosis‑associated lncRNAs and clinical factors. The optimal lncRNA combination was selected using the penalized package, and the risk‑score system was built and evaluated. After the lncRNA‑mRNA expression correlation network was constructed, the potential pathways involving the key lncRNAs were enriched using Gene Set Enrichment Analysis. Among the 61 DE‑lncRNAs, 48 lncRNAs were significantly associated with prognosis. Relapse was an independent prognostic factor. The optimal 14‑lncRNA risk score system was constructed. After 730 differentially expressed mRNAs were identified between the good and bad prognosis groups divided using a prognostic index, the lncRNA‑mRNA expression correlation network was constructed. Enrichment analysis showed that semaphorin‑3C [SEMA3C; regulated by probable leucine‑tRNA ligase, mitochondrial (LARS2‑AS1)] and secreted frizzled‑related protein 5 [SFRP5; mediated by WASH complex subunit 5 (WASHC5)‑antisense RNA 1 (AS1)] were involved in axon guidance and the Wnt signaling pathway, respectively. A 14‑lncRNA (including paired box protein Pax8‑AS1 and MYB AS1) risk‑score system might be effective in predicting the prognosis of AML. Axon guidance (involving SEMA3C and LARS2‑AS1) and the Wnt signaling pathway (involving SFRP5 and WASHC5‑AS1) might be two important pathways affecting the prognosis of AML.
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August-2020
Volume 20 Issue 2

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Guo S, Li B, Xu X, Wang W, Wang S, Lv T and Wang H: Construction of a 14‑lncRNA risk score system predicting survival of children with acute myelocytic leukemia. Exp Ther Med 20: 1521-1531, 2020
APA
Guo, S., Li, B., Xu, X., Wang, W., Wang, S., Lv, T., & Wang, H. (2020). Construction of a 14‑lncRNA risk score system predicting survival of children with acute myelocytic leukemia. Experimental and Therapeutic Medicine, 20, 1521-1531. https://doi.org/10.3892/etm.2020.8846
MLA
Guo, S., Li, B., Xu, X., Wang, W., Wang, S., Lv, T., Wang, H."Construction of a 14‑lncRNA risk score system predicting survival of children with acute myelocytic leukemia". Experimental and Therapeutic Medicine 20.2 (2020): 1521-1531.
Chicago
Guo, S., Li, B., Xu, X., Wang, W., Wang, S., Lv, T., Wang, H."Construction of a 14‑lncRNA risk score system predicting survival of children with acute myelocytic leukemia". Experimental and Therapeutic Medicine 20, no. 2 (2020): 1521-1531. https://doi.org/10.3892/etm.2020.8846