Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Experimental and Therapeutic Medicine
Join Editorial Board Propose a Special Issue
Print ISSN: 1792-0981 Online ISSN: 1792-1015
Journal Cover
August-2020 Volume 20 Issue 2

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
August-2020 Volume 20 Issue 2

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article Open Access

ZMIZ1 promotes the proliferation and migration of melanocytes in vitiligo

  • Authors:
    • Meng Li
    • Yibin Fan
    • Yutong Wang
    • Jinhua Xu
    • Hui Xu
  • View Affiliations / Copyright

    Affiliations: Department of Dermatology, Shanghai Ninth People's Hospital, Shanghai Jiaotong University, School of Medicine, Shanghai 200011, P.R. China, Department of Dermatology, Zhejiang Provincial People's Hospital, People's Hospital of Hangzhou Medical College, Hangzhou, Zhejiang 310014, P.R. China, Department of Dermatology, Huashan Hospital, Fudan University, Shanghai 200041, P.R. China
    Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1371-1378
    |
    Published online on: June 5, 2020
       https://doi.org/10.3892/etm.2020.8849
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Genome wide association studies have revealed that the zinc finger MIZ-type containing 1 (ZMIZ1) is involved in the pathogenesis of vitiligo; however, the underlying mechanism remains unclear. The present study aimed to investigate the effects of ZMIZ1 on the proliferation, apoptosis and migration of the human melanocyte cell lines PIG1 and PIG3V. ZMIZ1 overexpression and knockdown PIG1 and PIG3V cell models were established by lentivirus infection, and the effects of ZMIZ1 on cell proliferation and apoptosis were determined using an MTT assay and flow cytometry, respectively. Furthermore, the expression levels of proliferation- and apoptosis-associated proteins were analyzed using western blotting. Additionally, Transwell assays were performed to determine the effect of ZMIZ1 on the migration of PIG1 and PIG3V cells. Finally, the effect of ZMIZ1 on cytoskeletal remodeling in PIG1 and PIG3V cells was analyzed using immunocytochemistry. The overexpression of ZMIZ1 promoted the proliferation and inhibited the apoptosis of PIG1 and PIG3V cells, whereas the genetic knockdown of ZMIZ1 resulted in the opposite effects. Furthermore, ZMIZ1 overexpression increased the migration, whereas the knockdown of ZMIZ1 inhibited the migration and altered remodeling of the actin cytoskeleton in PIG1 and PIG3V cells. In conclusion, the results of the present study suggest that ZMIZ1 regulates the proliferation, apoptosis and migration of PIG1 and PIG3V cells, and indicate that ZMIZ1 may serve as a potential therapeutic target for vitiligo.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

View References

1 

Yadav AK, Singh P and Khunger N: Clinicopathologic analysis of stable and unstable vitiligo: A study of 66 cases. Am J Dermatopathol. 38:608–613. 2016.PubMed/NCBI View Article : Google Scholar

2 

Shi H, Lin B, Huang Y, Wu J, Zhang H, Lin C, Wang Z, Zhu J, Zhao Y, Fu X, et al: Basic fibroblast growth factor promotes melanocyte migration via activating PI3K/Akt-Rac1-FAK-JNK and ERK signaling pathways. IUBMB Life. 68:735–747. 2016.PubMed/NCBI View Article : Google Scholar

3 

Wang X, Du J, Wang T, Zhou C, Shen Y, Ding X, Tian S, Liu Y, Peng G, Xue S, et al: Prevalence and clinical profile of vitiligo in China: A community-based study in six cities. Acta Derm Venereol. 93:62–65. 2013.PubMed/NCBI View Article : Google Scholar

4 

Grimes PE and Miller MM: Vitiligo: Patient stories, self-esteem, and the psychological burden of disease. Int J Womens Dermatol. 4:32–37. 2018.PubMed/NCBI View Article : Google Scholar

5 

Hadi A, Wang JF, Uppal P, Penn LA and Elbuluk N: Comorbid diseases of vitiligo: A 10-year cross-sectional retrospective study of an urban US population. J Am Acad Dermatol. 82:628–633. 2020.PubMed/NCBI View Article : Google Scholar

6 

Yuan X, Meng D, Cao P, Sun L, Pang Y, Li Y, Wang X, Luo Z, Zhang L and Liu G: Identification of pathogenic genes and transcription factors in vitiligo. Dermatol Ther (Heidelb). 32(e13025)2019.PubMed/NCBI View Article : Google Scholar

7 

Galeone M, Colucci R, Dragoni F and Moretti S: Can environmental factors contribute in triggering vitiligo and associated autoimmune thyroid diseases? Clinical series possibly related to the Chernobyl nuclear accident. G Ital Dermatol Venereol. 153:729–730. 2018.PubMed/NCBI View Article : Google Scholar

8 

Laddha NC, Dwivedi M, Gani AR, Mansuri MS and Begum R: Tumor necrosis factor B (TNFB) genetic variants and its increased expression are associated with vitiligo susceptibility. PLoS One. 8(e81736)2013.PubMed/NCBI View Article : Google Scholar

9 

Al-Harthi F, Zouman A, Arfin M, Tariq M and Al-Asmari A: Tumor necrosis factor-α and -β genetic polymorphisms as a risk factor in Saudi patients with vitiligo. Genet Mol Res. 12:2196–2204. 2013.PubMed/NCBI View Article : Google Scholar

10 

Veiga-Castelli L, Oliveira ML, Pereira A, Debortoli G, Marcorin L, Fracasso N, Silva G, Souza A, Massaro J, Simões AL, et al: HLA-G polymorphisms are associated with non-segmental vitiligo among Brazilians. Biomolecules. 9(9)2019.PubMed/NCBI View Article : Google Scholar

11 

Yi X, Cui T, Li S, Yang Y, Chen J, Guo S, Jian Z, Li C, Gao T, Liu L, et al: Identification of the risk HLA-A alleles and autoantigen in Han Chinese vitiligo patients and the association of CD8+T cell reactivity with disease characteristics. Med Sci Monit. 24:6489–6497. 2018.PubMed/NCBI View Article : Google Scholar

12 

Li J, Yan M, Zhang Y, Feng C, Wang H, Wang C and Sun L: Meta-analysis of the association between NLRP1 polymorphisms and the susceptibility to vitiligo and associated autoimmune diseases. Oncotarget. 8:88179–88188. 2017.PubMed/NCBI View Article : Google Scholar

13 

Sun Y, Zuo X, Zheng X, Zhou F, Liang B, Liu H, Chang R, Gao J, Sheng Y, Cui H, et al: A comprehensive association analysis confirms ZMIZ1 to be a susceptibility gene for vitiligo in Chinese population. J Med Genet. 51:345–353. 2014.PubMed/NCBI View Article : Google Scholar

14 

Yang SK, Hong M, Choi H, Zhao W, Jung Y, Haritunians T, Ye BD, Kim KJ, Park SH, Lee I, et al: Immunochip analysis identification of 6 additional susceptibility loci for Crohn's disease in Koreans. Inflamm Bowel Dis. 21:1–7. 2015.PubMed/NCBI View Article : Google Scholar

15 

Rakowski LA, Garagiola DD, Li CM, Decker M, Caruso S, Jones M, Kuick R, Cierpicki T, Maillard I and Chiang MY: Convergence of the ZMIZ1 and NOTCH1 pathways at C-MYC in acute T lymphoblastic leukemias. Cancer Res. 73:930–941. 2013.PubMed/NCBI View Article : Google Scholar

16 

Peng Y, Lee J, Zhu C and Sun Z: A novel role for protein inhibitor of activated STAT (PIAS) proteins in modulating the activity of Zimp7, a novel PIAS-like protein, in androgen receptor-mediated transcription. J Biol Chem. 285:11465–11475. 2010.PubMed/NCBI View Article : Google Scholar

17 

Xu L, Zhang Z, Xie T, Zhang X and Dai T: Inhibition of BDNF-AS Provides Neuroprotection for Retinal Ganglion Cells against Ischemic Injury. PLoS One. 11(e0164941)2016.PubMed/NCBI View Article : Google Scholar

18 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. Methods. 25:402–408. 2001.PubMed/NCBI View Article : Google Scholar

19 

Schnoor M: Endothelial actin-binding proteins and actin dynamics in leukocyte transendothelial migration. J Immunol. 194:3535–3541. 2015.PubMed/NCBI View Article : Google Scholar

20 

Wang DG, Xu XH, Ma HJ, Li CR, Yue XZ, Gao J and Zhu WY: Stem cell factor combined with matrix proteins regulates the attachment and migration of melanocyte precursors of human hair follicles in vitro. Biol Pharm Bull. 36:1317–1325. 2013.PubMed/NCBI View Article : Google Scholar

21 

Thingnes J, Lavelle TJ, Gjuvsland AB, Omholt SW and Hovig E: Towards a quantitative understanding of the MITF-PIAS3-STAT3 connection. BMC Syst Biol. 6(11)2012.PubMed/NCBI View Article : Google Scholar

22 

Kim HJ, Choi CP, Uhm YK, Kim YI, Lee JW, Yoon SH, Chung JH and Lee MH: The association between endothelin-1 gene polymorphisms and susceptibility to vitiligo in a Korean population. Exp Dermatol. 16:561–566. 2007.PubMed/NCBI View Article : Google Scholar

23 

Jeong KH, Shin MK, Uhm YK, Kim HJ, Chung JH and Lee MH: Association of TXNDC5 gene polymorphisms and susceptibility to nonsegmental vitiligo in the Korean population. Br J Dermatol. 162:759–764. 2010.PubMed/NCBI View Article : Google Scholar

24 

Ben Ahmed M, Zaraa I, Rekik R, Elbeldi-Ferchiou A, Kourda N, Belhadj Hmida N, Abdeladhim M, Karoui O, Ben Osman A, Mokni M, et al: Functional defects of peripheral regulatory T lymphocytes in patients with progressive vitiligo. Pigment Cell Melanoma Res. 25:99–109. 2012.PubMed/NCBI View Article : Google Scholar

25 

Lan CC, Wu CS, Chiou MH, Chiang TY and Yu HS: Low-energy helium-neon laser induces melanocyte proliferation via interaction with type IV collagen: Visible light as a therapeutic option for vitiligo. Br J Dermatol. 161:273–280. 2009.PubMed/NCBI View Article : Google Scholar

26 

Goldstein NB, Koster MI, Hoaglin LG, Spoelstra NS, Kechris KJ, Robinson SE, Robinson WA, Roop DR, Norris DA and Birlea SA: Narrow band ultraviolet B treatment for human vitiligo is associated with proliferation, migration, and differentiation of melanocyte precursors. J Invest Dermatol. 135:2068–2076. 2015.PubMed/NCBI View Article : Google Scholar

27 

Song Y, Zhong M and Cai FC: Oxcarbazepine causes neurocyte apoptosis and developing brain damage by triggering Bax/Bcl-2 signaling pathway mediated caspase 3 activation in neonatal rats. Eur Rev Med Pharmacol Sci. 22:250–261. 2018.PubMed/NCBI View Article : Google Scholar

28 

Hu PF, Chen WP, Bao JP and Wu LD: Paeoniflorin inhibits IL-1β -induced chondrocyte apoptosis by regulating the Bax/Bcl-2/caspase-3 signaling pathway. Mol Med Rep. 17:6194–6200. 2018.PubMed/NCBI View Article : Google Scholar

29 

Keswell D, Davids LM and Kidson SH: Migration of human melanocytes into keratinocyte monolayers in vitro. J Dermatol Sci. 66:160–163. 2012.PubMed/NCBI View Article : Google Scholar

30 

AlGhamdi KM, Kumar A, Ashour AE and AlGhamdi AA: A comparative study of the effects of different low-level lasers on the proliferation, viability, and migration of human melanocytes in vitro. Lasers Med Sci. 30:1541–1551. 2015.PubMed/NCBI View Article : Google Scholar

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Li M, Fan Y, Wang Y, Xu J and Xu H: ZMIZ1 promotes the proliferation and migration of melanocytes in vitiligo. Exp Ther Med 20: 1371-1378, 2020.
APA
Li, M., Fan, Y., Wang, Y., Xu, J., & Xu, H. (2020). ZMIZ1 promotes the proliferation and migration of melanocytes in vitiligo. Experimental and Therapeutic Medicine, 20, 1371-1378. https://doi.org/10.3892/etm.2020.8849
MLA
Li, M., Fan, Y., Wang, Y., Xu, J., Xu, H."ZMIZ1 promotes the proliferation and migration of melanocytes in vitiligo". Experimental and Therapeutic Medicine 20.2 (2020): 1371-1378.
Chicago
Li, M., Fan, Y., Wang, Y., Xu, J., Xu, H."ZMIZ1 promotes the proliferation and migration of melanocytes in vitiligo". Experimental and Therapeutic Medicine 20, no. 2 (2020): 1371-1378. https://doi.org/10.3892/etm.2020.8849
Copy and paste a formatted citation
x
Spandidos Publications style
Li M, Fan Y, Wang Y, Xu J and Xu H: ZMIZ1 promotes the proliferation and migration of melanocytes in vitiligo. Exp Ther Med 20: 1371-1378, 2020.
APA
Li, M., Fan, Y., Wang, Y., Xu, J., & Xu, H. (2020). ZMIZ1 promotes the proliferation and migration of melanocytes in vitiligo. Experimental and Therapeutic Medicine, 20, 1371-1378. https://doi.org/10.3892/etm.2020.8849
MLA
Li, M., Fan, Y., Wang, Y., Xu, J., Xu, H."ZMIZ1 promotes the proliferation and migration of melanocytes in vitiligo". Experimental and Therapeutic Medicine 20.2 (2020): 1371-1378.
Chicago
Li, M., Fan, Y., Wang, Y., Xu, J., Xu, H."ZMIZ1 promotes the proliferation and migration of melanocytes in vitiligo". Experimental and Therapeutic Medicine 20, no. 2 (2020): 1371-1378. https://doi.org/10.3892/etm.2020.8849
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team