Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Experimental and Therapeutic Medicine
Join Editorial Board Propose a Special Issue
Print ISSN: 1792-0981 Online ISSN: 1792-1015
Journal Cover
August-2020 Volume 20 Issue 2

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
August-2020 Volume 20 Issue 2

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article Open Access

miR‑223‑3p reduces high glucose and high fat‑induced endothelial cell injury in diabetic mice by regulating NLRP3 expression

  • Authors:
    • Bo Deng
    • Ying Hu
    • Xia Sheng
    • Huijun Zeng
    • Yanan Huo
  • View Affiliations / Copyright

    Affiliations: Department of Endocrinology, The Third Affiliated Hospital of Nanchang University, Nanchang, Jiangxi 330008, P.R. China, Department of Pharmacy, The Third Affiliated Hospital of Nanchang University, Nanchang, Jiangxi 330008, P.R. China, Department of Endocrinology, Jiangxi Provincial People's Hospital, Nanchang, Jiangxi 330006, P.R. China
    Copyright: © Deng et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1514-1520
    |
    Published online on: June 10, 2020
       https://doi.org/10.3892/etm.2020.8864
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Expression levels of miR‑223‑3p and NLRP3 in high glucose and high fat (HGHF)‑induced diabetic mice, and the mechanism on the injury of mouse cardiac microvascular endothelial cells (MCMECs) were investigated. Four‑week C57BL/6J laboratory mice were selected and randomized into a control group and a model group (n=10 each). Mice in the model group were fed with HGHF diet to establish a mouse model of diabetes. Further MCMECs were purchased to construct carriers through transient transfection, and were separated into a normal group (cultured in the normal environment), a model group (not transfected), a blank carrier group (transfected with miR‑NC), a miR‑223‑3p‑mimics group, and a miR‑223‑3p‑inhibitor group. RT‑qPCR was used to detect the expression levels of miR‑223‑3p and NLRP3, and western blot analysis to detect the expression levels of NLRP3, apoptosis‑related proteins Bax and caspase‑3, and anti‑apoptotic protein Bcl‑2. Flow cytometry was used to observe apoptosis and TargetScan to predict the target relationship between miR‑223‑3p and NLRP3. Dual‑luciferase reporter gene assay was used to detect the relationship between miR‑223‑3p and NLRP3. Compared with those in the control group, the mice in the model group had significantly lower expression of miR‑223‑3p. However, significantly higher mRNA and protein expression levels of NLRP3 were observed (P<0.05). After modeling, miR‑223‑3p overexpression downregulated the expression levels of NLRP3 mRNA, Bax and NLRP3 protein, as well as inhibited endothelial cell apoptosis (P<0.05), while the inhibition of miR‑223‑3p expression upregulated the expression levels and promoted apoptosis. In conclusion, miR‑223‑3p expression is low, however, NLRP3 is highly expressed in the heart tissue of HGHF‑induced diabetic mice. miR‑223‑3p reduces the injury of MCMECs and inhibits endothelial cell apoptosis in mice by regulating the expression of NLRP3.
View Figures

Figure 1

Figure 2

Figure 3

View References

1 

Chamberlain JJ, Rhinehart AS, Shaefer CF Jr and Neuman A: Diagnosis and management of diabetes: Synopsis of the 2016 American Diabetes Association Standards of Medical Care in Diabetes. Ann Intern Med. 164:542–552. 2016.PubMed/NCBI View Article : Google Scholar

2 

Micha R, Peñalvo JL, Cudhea F, Imamura F, Rehm CD and Mozaffarian D: Association between dietary factors and mortality from heart disease, stroke, and type 2 diabetes in the United States. JAMA. 317:912–924. 2017.PubMed/NCBI View Article : Google Scholar

3 

Zhao W, Rasheed A, Tikkanen E, Lee JJ, Butterworth AS, Howson JMM, Assimes TL, Chowdhury R, Orho-Melander M, Damrauer S, et al: CHD Exome+ Consortium; EPIC-CVD Consortium; EPIC-Interact Consortium; Michigan Biobank: Identification of new susceptibility loci for type 2 diabetes and shared etiological pathways with coronary heart disease. Nat Genet. 49:1450–1457. 2017.PubMed/NCBI View Article : Google Scholar

4 

Parenti A, Pala L, Paccosi S and Rotella CM: Potential role for dendritic cells in endothelial dysfunction, diabetes and cardiovascular disease. Curr Pharm Des. 23:1435–1444. 2017.PubMed/NCBI View Article : Google Scholar

5 

Rawal S, Munasinghe PE, Shindikar A, Paulin J, Cameron V, Manning P, Williams MJ, Jones GT, Bunton R, Galvin I, et al: Down-regulation of proangiogenic microRNA-126 and microRNA-132 are early modulators of diabetic cardiac microangiopathy. Cardiovasc Res. 113:90–101. 2017.PubMed/NCBI View Article : Google Scholar

6 

Dai GH, Ma PZ, Song XB, Liu N, Zhang T and Wu B: MicroRNA-223-3p inhibits the angiogenesis of ischemic cardiac microvascular endothelial cells via affecting RPS6KB1/hif-1a signal pathway. PLoS One. 9(e108468)2014.PubMed/NCBI View Article : Google Scholar

7 

Liu X, Zhang Y, Du W, Liang H, He H, Zhang L, Pan Z, Li X, Xu C, Zhou Y, et al: MiR-223-3p as a novel microRNA regulator of expression of voltage-gated K+ channel Kv4.2 in acute myocardial infarction. Cell Physiol Biochem. 39:102–114. 2016.PubMed/NCBI View Article : Google Scholar

8 

Wang X, Huang W, Yang Y, Wang Y, Peng T, Chang J, Caldwell CC, Zingarelli B and Fan GC: Loss of duplex miR-223 (5p and 3p) aggravates myocardial depression and mortality in polymicrobial sepsis. Biochim Biophys Acta. 1842:701–711. 2014.PubMed/NCBI View Article : Google Scholar

9 

Yi H, Peng R, Zhang LY, Sun Y, Peng HM, Liu HD, Yu LJ, Li AL, Zhang YJ, Jiang WH, et al: LincRNA-Gm4419 knockdown ameliorates NF-κB/NLRP3 inflammasome-mediated inflammation in diabetic nephropathy. Cell Death Dis. 8(e2583)2017.PubMed/NCBI View Article : Google Scholar

10 

Stienstra R, van Diepen JA, Tack CJ, Zaki MH, van de Veerdonk FL, Perera D, Neale GA, Hooiveld GJ, Hijmans A, Vroegrijk I, et al: Inflammasome is a central player in the induction of obesity and insulin resistance. Proc Natl Acad Sci USA. 108:15324–15329. 2011.PubMed/NCBI View Article : Google Scholar

11 

Wang L, Chen Y, Li X and Zhang Y, Gulbins E and Zhang Y: Enhancement of endothelial permeability by free fatty acid through lysosomal cathepsin B-mediated Nlrp3 inflammasome activation. Oncotarget. 7:73229–73241. 2016.PubMed/NCBI View Article : Google Scholar

12 

Zhou W, Wang G, Zhao X, Xiong F, Zhou S, Peng J, Cheng Y, Xu S and Xu X: A multiplex qPCR gene dosage assay for rapid genotyping and large-scale population screening for deletional α-thalassemia. J Mol Diagn. 5:642–651. 2013.PubMed/NCBI View Article : Google Scholar

13 

Soliman EZ, Backlund JC, Bebu I, Orchard TJ, Zinman B and Lachin JM: DCCT/EDIC Research Group. Electrocardiographic abnormalities and cardiovascular disease risk in type 1 diabetes: The Epidemiology of Diabetes Interventions and Complications (EDIC) Study. Diabetes Care. 40:793–799. 2017.PubMed/NCBI View Article : Google Scholar

14 

Trikkalinou A, Papazafiropoulou AK and Melidonis A: Type 2 diabetes and quality of life. World J Diabetes. 8:120–129. 2017.PubMed/NCBI View Article : Google Scholar

15 

Cai H, Li G, Zhang P, Xu D and Chen L: Effect of exercise on the quality of life in type 2 diabetes mellitus: A systematic review. Qual Life Res. 26:515–530. 2017.PubMed/NCBI View Article : Google Scholar

16 

Guo R and Nair S: Role of microRNA in diabetic cardiomyopathy: From mechanism to intervention. Biochim Biophys Acta Mol Basis Dis. 1863:2070–2077. 2017.PubMed/NCBI View Article : Google Scholar

17 

Ye Y, Bajaj M, Yang HC, Perez-Polo JR and Birnbaum Y: SGLT-2 inhibition with dapagliflozin reduces the activation of the Nlrp3/ASC inflammasome and attenuates the development of diabetic cardiomyopathy in mice with type 2 diabetes. Further augmentation of the effects with saxagliptin, a DPP4 inhibitor. Cardiovasc Drugs Ther. 31:119–132. 2017.PubMed/NCBI View Article : Google Scholar

18 

Yang X, Lu F, Li L, Li J, Luo J, Zhang S, Liu X and Chen G: Wu-Mei-wan protects pancreatic β cells by inhibiting NLRP3 inflammasome activation in diabetic mice. BMC Complement Altern Med. 19(35)2019.PubMed/NCBI View Article : Google Scholar

19 

Liu Y, Lian K, Zhang L, Wang R, Yi F, Gao C, Xin C, Zhu D, Li Y, Yan W, et al: TXNIP mediates NLRP3 inflammasome activation in cardiac microvascular endothelial cells as a novel mechanism in myocardial ischemia/reperfusion injury. Basic Res Cardiol. 109(415)2014.PubMed/NCBI View Article : Google Scholar

20 

Chu M, Wu R, Qin S, Hua W, Shan Z, Rong X, Zeng J, Hong L, Sun Y, Liu Y, et al: Bone marrow-derived microRNA-223 works as an endocrine genetic signal in vascular endothelial cells and participates in vascular injury from Kawasaki disease. J Am Heart Assoc. 6(e004878)2017.PubMed/NCBI View Article : Google Scholar

21 

Zhang J, Xia L, Zhang F, Zhu D, Xin C, Wang H, Zhang F, Guo X, Lee Y, Zhang L, et al: A novel mechanism of diabetic vascular endothelial dysfunction: Hypoadiponectinemia-induced NLRP3 inflammasome activation. Biochim Biophys Acta Mol Basis Dis. 1863:1556–1567. 2017.PubMed/NCBI View Article : Google Scholar

22 

Castro-Villegas C, Pérez-Sánchez C, Escudero A, Filipescu I, Verdu M, Ruiz-Limón P, Aguirre MA, Jiménez-Gomez Y, Font P, Rodriguez-Ariza A, et al: Circulating miRNAs as biomarkers of therapy effectiveness in rheumatoid arthritis patients treated with anti-TNFα. Arthritis Res Ther. 17(49)2015.PubMed/NCBI View Article : Google Scholar

23 

Akushevich I, Yashkin AP, Kravchenko J, Fang F, Arbeev K, Sloan F and Yashin AI: Identifying the causes of the changes in the prevalence patterns of diabetes in older U.S. adults: A new trend partitioning approach. J Diabetes Complications. 32:362–367. 2018.PubMed/NCBI View Article : Google Scholar

24 

Patterson R, McNamara E, Tainio M, de Sá TH, Smith AD, Sharp SJ, Edwards P, Woodcock J, Brage S and Wijndaele K: Sedentary behaviour and risk of all-cause, cardiovascular and cancer mortality, and incident type 2 diabetes: A systematic review and dose response meta-analysis. Eur J Epidemiol. 33:811–829. 2018.PubMed/NCBI View Article : Google Scholar

25 

Kowluru RA, Kowluru V, Xiong Y and Ho YS: Overexpression of mitochondrial superoxide dismutase in mice protects the retina from diabetes-induced oxidative stress. Free Radic Biol Med. 41:1191–1196. 2006.PubMed/NCBI View Article : Google Scholar

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Deng B, Hu Y, Sheng X, Zeng H and Huo Y: miR‑223‑3p reduces high glucose and high fat‑induced endothelial cell injury in diabetic mice by regulating NLRP3 expression. Exp Ther Med 20: 1514-1520, 2020.
APA
Deng, B., Hu, Y., Sheng, X., Zeng, H., & Huo, Y. (2020). miR‑223‑3p reduces high glucose and high fat‑induced endothelial cell injury in diabetic mice by regulating NLRP3 expression. Experimental and Therapeutic Medicine, 20, 1514-1520. https://doi.org/10.3892/etm.2020.8864
MLA
Deng, B., Hu, Y., Sheng, X., Zeng, H., Huo, Y."miR‑223‑3p reduces high glucose and high fat‑induced endothelial cell injury in diabetic mice by regulating NLRP3 expression". Experimental and Therapeutic Medicine 20.2 (2020): 1514-1520.
Chicago
Deng, B., Hu, Y., Sheng, X., Zeng, H., Huo, Y."miR‑223‑3p reduces high glucose and high fat‑induced endothelial cell injury in diabetic mice by regulating NLRP3 expression". Experimental and Therapeutic Medicine 20, no. 2 (2020): 1514-1520. https://doi.org/10.3892/etm.2020.8864
Copy and paste a formatted citation
x
Spandidos Publications style
Deng B, Hu Y, Sheng X, Zeng H and Huo Y: miR‑223‑3p reduces high glucose and high fat‑induced endothelial cell injury in diabetic mice by regulating NLRP3 expression. Exp Ther Med 20: 1514-1520, 2020.
APA
Deng, B., Hu, Y., Sheng, X., Zeng, H., & Huo, Y. (2020). miR‑223‑3p reduces high glucose and high fat‑induced endothelial cell injury in diabetic mice by regulating NLRP3 expression. Experimental and Therapeutic Medicine, 20, 1514-1520. https://doi.org/10.3892/etm.2020.8864
MLA
Deng, B., Hu, Y., Sheng, X., Zeng, H., Huo, Y."miR‑223‑3p reduces high glucose and high fat‑induced endothelial cell injury in diabetic mice by regulating NLRP3 expression". Experimental and Therapeutic Medicine 20.2 (2020): 1514-1520.
Chicago
Deng, B., Hu, Y., Sheng, X., Zeng, H., Huo, Y."miR‑223‑3p reduces high glucose and high fat‑induced endothelial cell injury in diabetic mice by regulating NLRP3 expression". Experimental and Therapeutic Medicine 20, no. 2 (2020): 1514-1520. https://doi.org/10.3892/etm.2020.8864
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team