Open Access

Preparation and in vitro study of stromal cell‑derived factor 1‑targeted Fe3O4/poly(lactic‑co‑glycolic acid)/perfluorohexane nanoparticles

  • Authors:
    • Fei Wang
    • Zhigang Wang
    • Liang Pang
    • Shuliang Wan
    • Lihua Qiu
  • View Affiliations

  • Published online on: June 24, 2020     https://doi.org/10.3892/etm.2020.8925
  • Pages: 2003-2012
  • Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Compared with traditional imaging techniques, multimodal imaging obtains more accurate images that may increase disease detection rates. The present study prepared stromal cell‑derived factor 1 (SDF‑1)‑loaded, targeted nanoparticles coated with iron (II,III) oxide and perfluorohexane (PFH) to be used as polymer‑shelled contrast agents with multimodal imaging functions, with the aim of improving tongue cancer and lymph node metastasis diagnosis. The multifunctional, targeted, polymeric nanoparticles were prepared using a double emulsion method and chemokine SDF‑1 was conjugated to nanoparticles by a sulfide bond. The nanoparticles were spherical, uniform size and well dispersed. The results of the in vitro photoacoustic and ultrasonic imaging experiments demonstrated that the multifunctional nanoparticles displayed excellent multimodal imaging functions, as even small concentrations of nanoparticles presented clear ultrasound and photoacoustic imaging. When the temperature reached the boiling point of PFH (56˚C), a liquid‑gas phase change occurred and the microsphere volume and acoustic impedance increased, leading to enhanced ultrasonic development. The nanoparticles were automatically targeted to tongue squamous carcinoma cells in vitro via SDF‑1‑CXC chemokine receptor 4 interactions. The targeted experiment and flow cytometry results indicated that the nanoparticles underwent strong targeted binding to human tongue squamous cell carcinoma (SCC‑15) cells. In summary, the nanoparticles were automatically targeted to SCC‑15 cells and displayed promising characteristics for ultrasound and photoacoustic imaging. Higher concentrations of nanoparticles was associated with clearer imaged and greater echo intensity value and photoacoustic value. The present study established a foundation for the development of procedures for primary tongue cancer and lymph node metastasis diagnosis.

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September-2020
Volume 20 Issue 3

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Wang F, Wang Z, Pang L, Wan S and Qiu L: Preparation and in vitro study of stromal cell‑derived factor 1‑targeted Fe3O4/poly(lactic‑co‑glycolic acid)/perfluorohexane nanoparticles. Exp Ther Med 20: 2003-2012, 2020
APA
Wang, F., Wang, Z., Pang, L., Wan, S., & Qiu, L. (2020). Preparation and in vitro study of stromal cell‑derived factor 1‑targeted Fe3O4/poly(lactic‑co‑glycolic acid)/perfluorohexane nanoparticles. Experimental and Therapeutic Medicine, 20, 2003-2012. https://doi.org/10.3892/etm.2020.8925
MLA
Wang, F., Wang, Z., Pang, L., Wan, S., Qiu, L."Preparation and in vitro study of stromal cell‑derived factor 1‑targeted Fe3O4/poly(lactic‑co‑glycolic acid)/perfluorohexane nanoparticles". Experimental and Therapeutic Medicine 20.3 (2020): 2003-2012.
Chicago
Wang, F., Wang, Z., Pang, L., Wan, S., Qiu, L."Preparation and in vitro study of stromal cell‑derived factor 1‑targeted Fe3O4/poly(lactic‑co‑glycolic acid)/perfluorohexane nanoparticles". Experimental and Therapeutic Medicine 20, no. 3 (2020): 2003-2012. https://doi.org/10.3892/etm.2020.8925