Open Access

Circular RNA‑ABCB10 promotes angiogenesis induced by conditioned medium from human amnion‑derived mesenchymal stem cells via the microRNA‑29b‑3p/vascular endothelial growth factor A axis

  • Authors:
    • Zichun Tang
    • Junling Tan
    • Xiaoqin Yuan
    • Qianwen Zhou
    • Zhiyao Yuan
    • Ning Chen
    • Ming Shen
  • View Affiliations

  • Published online on: June 25, 2020     https://doi.org/10.3892/etm.2020.8939
  • Pages: 2021-2030
  • Copyright: © Tang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The powerful ability of human amnion-derived mesenchymal stem cells (hAMSCs) to promote angiogenesis suggests that they may facilitate angiogenesis‑associated therapeutic strategies. However, the molecular mechanisms underlying hAMSC‑induced angiogenesis remain largely unknown. The present study results suggested that enhanced migration and tube formation in human umbilical vein endothelial cells (HUVECs) was induced by conditioned medium from hAMSCs (hAMSC‑CM). In addition, culture with this conditioned medium resulted in the increased expression of circular RNA ATP binding cassette subfamily B member 10 (circ‑ABCB10) and vascular endothelial growth factor A (VEGFA). In the present study genes related to thecirc‑ABCB10/microRNA (miR)‑29b‑3p/VEGFA pathway were predicted using bioinformatics software, and further investigated using in vitro luciferase reporter assays. Loss‑of‑function assays were performed using small interfering RNAs (siRNAs). The results suggested that siRNA‑silencing of circ‑ABCB10 in HUVECs weakened migration and tube formation of HUVECs following hAMSC‑CM treatment and reduced the levels of VEGFA expression. Treatment with an miR‑29b‑3p inhibitor could largely rescue these effects in HUVECs, following circ‑ABCB10 silencing. The present study results suggest that the circ‑ABCB10/miR‑29b‑3p/VEGFA pathway may be involved in the pro‑angiogenic role of hAMSC‑CM in HUVECs.

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September-2020
Volume 20 Issue 3

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Tang Z, Tan J, Yuan X, Zhou Q, Yuan Z, Chen N and Shen M: Circular RNA‑ABCB10 promotes angiogenesis induced by conditioned medium from human amnion‑derived mesenchymal stem cells via the microRNA‑29b‑3p/vascular endothelial growth factor A axis. Exp Ther Med 20: 2021-2030, 2020
APA
Tang, Z., Tan, J., Yuan, X., Zhou, Q., Yuan, Z., Chen, N., & Shen, M. (2020). Circular RNA‑ABCB10 promotes angiogenesis induced by conditioned medium from human amnion‑derived mesenchymal stem cells via the microRNA‑29b‑3p/vascular endothelial growth factor A axis. Experimental and Therapeutic Medicine, 20, 2021-2030. https://doi.org/10.3892/etm.2020.8939
MLA
Tang, Z., Tan, J., Yuan, X., Zhou, Q., Yuan, Z., Chen, N., Shen, M."Circular RNA‑ABCB10 promotes angiogenesis induced by conditioned medium from human amnion‑derived mesenchymal stem cells via the microRNA‑29b‑3p/vascular endothelial growth factor A axis". Experimental and Therapeutic Medicine 20.3 (2020): 2021-2030.
Chicago
Tang, Z., Tan, J., Yuan, X., Zhou, Q., Yuan, Z., Chen, N., Shen, M."Circular RNA‑ABCB10 promotes angiogenesis induced by conditioned medium from human amnion‑derived mesenchymal stem cells via the microRNA‑29b‑3p/vascular endothelial growth factor A axis". Experimental and Therapeutic Medicine 20, no. 3 (2020): 2021-2030. https://doi.org/10.3892/etm.2020.8939