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Article Open Access

Role of microRNA‑15a‑5p/TNFAIP3‑interacting protein 2 axis in acute lung injury induced by traumatic hemorrhagic shock

  • Authors:
    • Feng Zhou
    • Zhizhen Liu
    • Huazhong Cai
    • Zhenjun Miao
    • Faxing Wei
    • Chao Song
  • View Affiliations / Copyright

    Affiliations: Department of Emergency, Affiliated Hospital of Jiangsu University, Zhenjiang, Jiangsu 212001, P.R. China
    Copyright: © Zhou et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 2
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    Published online on: August 25, 2020
       https://doi.org/10.3892/etm.2020.9130
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Abstract

The present study aimed to investigate the role of microRNA (miR)‑15a‑5p in the pathogenesis of acute lung injury induced by traumatic hemorrhagic shock (THS), and to explore the underlying molecular mechanism. The expression level of miR‑15a‑5p was detected using reverse transcription‑quantitative (RT‑qPCR) and the association between miR‑15a‑5p and TNFAIP3‑interacting protein 2 (TNIP2) was revealed using TargetScan and dual luciferase reporter assays. To investigate the effect of miR‑15a‑5p on THS‑induced acute lung injury, a THS rat model was established. Lung capillary permeability and lung edema were then determined. Moreover, proinflammatory factors in the bronchoalveolar lavage fluid (BALF) and serum of the THS rat model were detected using ELISA. In addition, protein levels in the current study were measured via western blotting. It was revealed that miR‑15a‑5p was significantly upregulated in both patients with THS and samples from the THS rat model. TNIP2 represents a direct target of miR‑15a‑5p, and it was downregulated in both patients with THS and the THS rat model. Further analyses indicated that downregulation of miR‑15a‑5p significantly relieved acute lung injury induced by THS, evidenced by a decreased ratio of Evan's blue dye (EBD) in the BALF to EBD in plasma of THS rats, decreased lung permeability index and reduced lung wet/dry ratio. Inhibition of miR‑15a‑5p also decreased THS‑induced upregulation of pro‑inflammatory factors. Furthermore, the data revealed that THS‑induced NF‑κB activation in the lung tissues of rats was inhibited by miR‑15a‑5p knockdown. Moreover, it was demonstrated that all the effects of miR‑15a‑5p on THS rats were ablated following TNIP2 silencing. Taken together, the data of the current study indicate that miR‑15a‑5p downregulation serves a protective role in THS‑induced acute lung injury via directly targeting TNIP2.
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Copy and paste a formatted citation
Spandidos Publications style
Zhou F, Liu Z, Cai H, Miao Z, Wei F and Song C: Role of microRNA‑15a‑5p/TNFAIP3‑interacting protein 2 axis in acute lung injury induced by traumatic hemorrhagic shock. Exp Ther Med 20: 2, 2020.
APA
Zhou, F., Liu, Z., Cai, H., Miao, Z., Wei, F., & Song, C. (2020). Role of microRNA‑15a‑5p/TNFAIP3‑interacting protein 2 axis in acute lung injury induced by traumatic hemorrhagic shock. Experimental and Therapeutic Medicine, 20, 2. https://doi.org/10.3892/etm.2020.9130
MLA
Zhou, F., Liu, Z., Cai, H., Miao, Z., Wei, F., Song, C."Role of microRNA‑15a‑5p/TNFAIP3‑interacting protein 2 axis in acute lung injury induced by traumatic hemorrhagic shock". Experimental and Therapeutic Medicine 20.5 (2020): 2.
Chicago
Zhou, F., Liu, Z., Cai, H., Miao, Z., Wei, F., Song, C."Role of microRNA‑15a‑5p/TNFAIP3‑interacting protein 2 axis in acute lung injury induced by traumatic hemorrhagic shock". Experimental and Therapeutic Medicine 20, no. 5 (2020): 2. https://doi.org/10.3892/etm.2020.9130
Copy and paste a formatted citation
x
Spandidos Publications style
Zhou F, Liu Z, Cai H, Miao Z, Wei F and Song C: Role of microRNA‑15a‑5p/TNFAIP3‑interacting protein 2 axis in acute lung injury induced by traumatic hemorrhagic shock. Exp Ther Med 20: 2, 2020.
APA
Zhou, F., Liu, Z., Cai, H., Miao, Z., Wei, F., & Song, C. (2020). Role of microRNA‑15a‑5p/TNFAIP3‑interacting protein 2 axis in acute lung injury induced by traumatic hemorrhagic shock. Experimental and Therapeutic Medicine, 20, 2. https://doi.org/10.3892/etm.2020.9130
MLA
Zhou, F., Liu, Z., Cai, H., Miao, Z., Wei, F., Song, C."Role of microRNA‑15a‑5p/TNFAIP3‑interacting protein 2 axis in acute lung injury induced by traumatic hemorrhagic shock". Experimental and Therapeutic Medicine 20.5 (2020): 2.
Chicago
Zhou, F., Liu, Z., Cai, H., Miao, Z., Wei, F., Song, C."Role of microRNA‑15a‑5p/TNFAIP3‑interacting protein 2 axis in acute lung injury induced by traumatic hemorrhagic shock". Experimental and Therapeutic Medicine 20, no. 5 (2020): 2. https://doi.org/10.3892/etm.2020.9130
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