Long non‑coding RNA Mirt2 interacts with long non‑coding RNA IFNG‑AS1 to regulate ulcerative colitis

  • Authors:
    • Chenyang Li
    • Lujia Cui
    • Siqiong Li
    • Minrui Li
    • Xinpu Miao
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  • Published online on: September 1, 2020     https://doi.org/10.3892/etm.2020.9159
  • Article Number: 32
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Abstract

Long non‑coding RNAs (lncRNAs) Mirt2 and interferon‑γ antisense RNA I (IFNG‑AS1) play opposing roles in lipopolysaccharide (LPS)‑induced inflammation, a key initiator of ulcerative colitis (UC). The present study aimed to analyze the potential interaction between Mirt2 and IFNG‑AS1 in UC. Levels of IFNG‑AS1 and Mirt2 in plasma samples from UC patients were measured using reverse transcription‑quantitative PCR. Receiver operating characteristic curves were used to evaluate the diagnostic values of IFNG‑AS1 and Mirt2 fr UC. The role of Mirt2 and IFNG‑AS1 in colonic epithelial cell apoptosis was analyzed by cell apoptosis assay. In patients with UC, Mirt2 and IFNG‑AS1 exhibited an inverse correlation, in which Mirt2 was downregulated while IFNG‑AS1 was upregulated. Altered expression of IFNG‑AS1 and Mirt2 separated patients with UC from healthy controls. In colonic epithelial cells, lipopolysaccharide treatment led to the downregulation of Mirt2 and the upregulation of IFNG‑AS1. Furthermore, overexpression of Mirt2 in colonic epithelial cells resulted in downregulation of IFNG‑AS1, and vice versa. Overexpression of Mirt2 led to a decreased rate of colonic epithelial cell apoptosis, while overexpression of IFNG‑AS1 led to an increased rate of apoptosis. Moreover, IFNG‑AS1 overexpression attenuated the effects of Mirt2 overexpression. Therefore, Mirt2 may interact with IFNG‑AS1 during UC to participate in colonic epithelial cell apoptosis.
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November-2020
Volume 20 Issue 5

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Li C, Cui L, Li S, Li M and Miao X: Long non‑coding RNA Mirt2 interacts with long non‑coding RNA IFNG‑AS1 to regulate ulcerative colitis. Exp Ther Med 20: 32, 2020
APA
Li, C., Cui, L., Li, S., Li, M., & Miao, X. (2020). Long non‑coding RNA Mirt2 interacts with long non‑coding RNA IFNG‑AS1 to regulate ulcerative colitis. Experimental and Therapeutic Medicine, 20, 32. https://doi.org/10.3892/etm.2020.9159
MLA
Li, C., Cui, L., Li, S., Li, M., Miao, X."Long non‑coding RNA Mirt2 interacts with long non‑coding RNA IFNG‑AS1 to regulate ulcerative colitis". Experimental and Therapeutic Medicine 20.5 (2020): 32.
Chicago
Li, C., Cui, L., Li, S., Li, M., Miao, X."Long non‑coding RNA Mirt2 interacts with long non‑coding RNA IFNG‑AS1 to regulate ulcerative colitis". Experimental and Therapeutic Medicine 20, no. 5 (2020): 32. https://doi.org/10.3892/etm.2020.9159