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Integrated Bioinformatics analysis and clinical validation reveals that high expression of mucin 1 in intrahepatic cholangiocarcinoma predicts recurrence after curative resection

  • Authors:
    • Fei-Yu Chen
    • Cheng Zhou
    • Xiang-Yu Zhang
    • Kai-Qian Zhou
    • Yuan-Fei Peng
    • Lei Yu
    • Jia Fan
    • Jian Zhou
    • Jie Hu
    • Zheng Wang
  • View Affiliations / Copyright

    Affiliations: Department of Liver Surgery, Liver Cancer Institute, Zhongshan Hospital, Fudan University, Key Laboratory of Carcinogenesis and Cancer Invasion, Fudan University, Ministry of Education, Shanghai 200032, P.R. China
    Copyright: © Chen et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 50
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    Published online on: September 3, 2020
       https://doi.org/10.3892/etm.2020.9178
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Abstract

Intrahepatic cholangiocarcinoma (ICC) is a cancer type with high malignancy and a current lack of biomarkers to predict recurrence. In the present study, to identify potential biomarkers, five ICC datasets from the Gene Expression Omnibus database were analyzed to construct initial datasets by using a robust rank aggregation approach. A total of 19 upregulated genes were identified in the initial datasets. The genes identified were then further analysed using data from The Cancer Genome Atlas. Only mucin 1 (MUC1) exhibited significance regarding differential expression and survival prediction. Finally, the expression levels of MUC1 were assessed using reverse transcription‑quantitative PCR in 61 pairs of ICC tumor and matched non‑cancerous samples. The expression of MUC1 was significantly elevated in ICC tissues compared with that in matched non‑cancerous counterparts (P=0.001). Patients with high MUC1 expression levels had significantly shorter overall survival (OS, P=0.009) and recurrence‑free survival (RFS, P=0.012). MUC1 was identified as an independent prognostic factor for OS [hazard ratio (HR)=2.364, 95%CI: 1.214‑4.485; P=0.023] and RFS (HR=2.552 95%CI: 1.294‑5.032; P=0.007) in the multivariate analysis. Using receiver operating characteristic analysis, a co‑index including MUC1 had a high accuracy for predicting survival [MUC1 combined with serum levels of CEA and cancer antigen 19‑9, and lymph node metastasis, area under curve (AUC)=0.746, 95%CI: 0.620‑0.872] and recurrence (MUC1 combined with bile duct invasion and lymph node metastasis, AUC=0.729, 95%CI: 0.605‑854). In conclusion, MUC1 is highly expressed in ICC tissue and is a potential prognostic biomarker and therapeutic target for ICC.
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Copy and paste a formatted citation
Spandidos Publications style
Chen F, Zhou C, Zhang X, Zhou K, Peng Y, Yu L, Fan J, Zhou J, Hu J, Wang Z, Wang Z, et al: Integrated Bioinformatics analysis and clinical validation reveals that high expression of mucin 1 in intrahepatic cholangiocarcinoma predicts recurrence after curative resection. Exp Ther Med 20: 50, 2020.
APA
Chen, F., Zhou, C., Zhang, X., Zhou, K., Peng, Y., Yu, L. ... Wang, Z. (2020). Integrated Bioinformatics analysis and clinical validation reveals that high expression of mucin 1 in intrahepatic cholangiocarcinoma predicts recurrence after curative resection. Experimental and Therapeutic Medicine, 20, 50. https://doi.org/10.3892/etm.2020.9178
MLA
Chen, F., Zhou, C., Zhang, X., Zhou, K., Peng, Y., Yu, L., Fan, J., Zhou, J., Hu, J., Wang, Z."Integrated Bioinformatics analysis and clinical validation reveals that high expression of mucin 1 in intrahepatic cholangiocarcinoma predicts recurrence after curative resection". Experimental and Therapeutic Medicine 20.5 (2020): 50.
Chicago
Chen, F., Zhou, C., Zhang, X., Zhou, K., Peng, Y., Yu, L., Fan, J., Zhou, J., Hu, J., Wang, Z."Integrated Bioinformatics analysis and clinical validation reveals that high expression of mucin 1 in intrahepatic cholangiocarcinoma predicts recurrence after curative resection". Experimental and Therapeutic Medicine 20, no. 5 (2020): 50. https://doi.org/10.3892/etm.2020.9178
Copy and paste a formatted citation
x
Spandidos Publications style
Chen F, Zhou C, Zhang X, Zhou K, Peng Y, Yu L, Fan J, Zhou J, Hu J, Wang Z, Wang Z, et al: Integrated Bioinformatics analysis and clinical validation reveals that high expression of mucin 1 in intrahepatic cholangiocarcinoma predicts recurrence after curative resection. Exp Ther Med 20: 50, 2020.
APA
Chen, F., Zhou, C., Zhang, X., Zhou, K., Peng, Y., Yu, L. ... Wang, Z. (2020). Integrated Bioinformatics analysis and clinical validation reveals that high expression of mucin 1 in intrahepatic cholangiocarcinoma predicts recurrence after curative resection. Experimental and Therapeutic Medicine, 20, 50. https://doi.org/10.3892/etm.2020.9178
MLA
Chen, F., Zhou, C., Zhang, X., Zhou, K., Peng, Y., Yu, L., Fan, J., Zhou, J., Hu, J., Wang, Z."Integrated Bioinformatics analysis and clinical validation reveals that high expression of mucin 1 in intrahepatic cholangiocarcinoma predicts recurrence after curative resection". Experimental and Therapeutic Medicine 20.5 (2020): 50.
Chicago
Chen, F., Zhou, C., Zhang, X., Zhou, K., Peng, Y., Yu, L., Fan, J., Zhou, J., Hu, J., Wang, Z."Integrated Bioinformatics analysis and clinical validation reveals that high expression of mucin 1 in intrahepatic cholangiocarcinoma predicts recurrence after curative resection". Experimental and Therapeutic Medicine 20, no. 5 (2020): 50. https://doi.org/10.3892/etm.2020.9178
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