miR‑590‑5p may regulate colorectal cancer cell viability and migration by targeting PDCD4 Corrigendum in /10.3892/etm.2024.12392

Guo,Ting; Wang,Jun; Cheng,Guochang; Huang,He

doi:10.3892/etm.2020.9183

miR‑590‑5p may regulate colorectal cancer cell viability and migration by targeting PDCD4

Corrigendum in: /10.3892/etm.2024.12392

Authors:
- Ting Guo
- Jun Wang
- Guochang Cheng
- He Huang
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Affiliations: Central Laboratory, Taizhou People's Hospital, Taizhou, Jiangsu 225300, P.R. China, Department of Emergency, Taizhou People's Hospital, Taizhou, Jiangsu 225300, P.R. China, Department of Surgery, Taizhou People's Hospital, Taizhou, Jiangsu 225300, P.R. China, Department of Oncology, Taizhou People's Hospital, Taizhou, Jiangsu 225300, P.R. China
Published online on: September 4, 2020 https://doi.org/10.3892/etm.2020.9183
Article Number: 55
Copyright: © Guo et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Recent studies have revealed that microRNAs (miRs) are involved in the pathogenesis of colorectal cancer (CRC); however, the roles of miR‑590‑5p in CRC are not completely understood. Therefore, the present study investigated the expression of miR‑590‑5p and programmed cell death 4 (PDCD4) in CRC tissues and healthy adjacent tissues via reverse transcription‑quantitative PCR. Furthermore, human CRC cells were cultured in vitro and transfected with miR‑590‑5p inhibitor. CRC cell viability, migration and invasion were evaluated by conducting MTT, wound healing and Transwell assays, respectively. Additionally, the relative expression of PDCD4 and phosphorylated‑Smad2/3 was analyzed via western blotting. miR‑590‑5p was significantly upregulated and PDCD4 was significantly downregulated in CRC tissues compared with healthy adjacent tissues. Moreover, the results indicated that miR‑590‑5p knockdown inhibited cell viability and migration by altering the expression of PDCD4, transforming growth factor‑β and phosphorylated‑Smad2/3. PDCD4 was identified as a direct target of miR‑590‑5p. In conclusion, the results of the present study suggested that miR‑590‑5p may regulate CRC cell viability and migration, indicating that miR‑590‑5p may serve as a potential therapeutic target for CRC.

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1	Jacobsen F, Kraft J, Schroeder C, Hube-Magg C, Kluth M, Lang DS, Simon R, Sauter G, Izbicki JR, Clauditz TS, et al: Up-regulation of biglycan is associated with poor prognosis and PTEN deletion in patients with prostate cancer. Neoplasia. 19:707–715. 2017.PubMed/NCBI View Article : Google Scholar
2	Anderson JC and Levine JB: Age and crc risk in the serrated pathway. J Clin Gastroenterol. 52:465–467. 2018.PubMed/NCBI View Article : Google Scholar
3	Wang G, Fu Y, Hu F, Lan J, Xu F, Yang X, Luo X, Wang J and Hu J: Loss of BRG1 induces CRC cell senescence by regulating p53/p21 pathway. Cell Death Dis. 8(e2607)2017.PubMed/NCBI View Article : Google Scholar
4	Orang AV and Barzegari A: MicroRNAs in colorectal cancer: From diagnosis to targeted therapy. Asian Pac J Cancer Prev. 15:6989–6999. 2014.PubMed/NCBI View Article : Google Scholar
5	Sun ZQ, Shi K, Zhou QB, Zeng XY, Liu J, Yang SX, Wang QS, Li Z, Wang GX, Song JM, et al: MiR-590-3p promotes proliferation and metastasis of colorectal cancer via Hippo pathway. Oncotarget. 8:58061–58071. 2017.PubMed/NCBI View Article : Google Scholar
6	Chen M, Li D, Gong N, Wu H, Su C, Xie C, Xiang H, Lin C and Li X: miR-133b down-regulates ABCC1 and enhances the sensitivity of CRC to anti-tumor drugs. Oncotarget. 8:52983–52994. 2017.PubMed/NCBI View Article : Google Scholar
7	Guglielmo A, Staropoli N, Giancotti M and Mauro M: Personalized medicine in colorectal cancer diagnosis and treatment: A systematic review of health economic evaluations. Cost Eff Resour Alloc. 16(2)2018.PubMed/NCBI View Article : Google Scholar
8	Dekker E and IJspeert JEG: Serrated pathway: A paradigm shift in CRC prevention. Gut. 67:1751–1752. 2018.PubMed/NCBI View Article : Google Scholar
9	Park YR, Lee ST, Kim SL, Zhu SM, Lee MR, Kim SH, Kim IH, Lee SO, Seo SY and Kim SW: Down-regulation of miR-9 promotes epithelial mesenchymal transition via regulating anoctamin-1 (ANO1) in CRC cells. Cancer Genet. 231-232:22–31. 2019.PubMed/NCBI View Article : Google Scholar
10	Zhang Z, Li J, Huang Y, Peng W, Qian W, Gu J, Wang Q, Hu T, Ji D, Ji B, et al: Upregulated miR-1258 regulates cell cycle and inhibits cell proliferation by directly targeting E2F8 in CRC. Cell Prolif. 51(e12505)2018.PubMed/NCBI View Article : Google Scholar
11	Tong F, Ying Y, Pan H, Zhao W, Li H and Zhan X: MicroRNA-466 (miR-466) functions as a tumor suppressor and prognostic factor in colorectal cancer (CRC). Bosn J Basic Med Sci. 18:252–259. 2018.PubMed/NCBI View Article : Google Scholar
12	Liu X and Cui M: MiRNA-98-5p inhibits the progression of osteosarcoma by regulating cell cycle via targeting CDC25A expression. Eur Rev Med Pharmacol Sci. 23:9793–9802. 2019.PubMed/NCBI View Article : Google Scholar
13	Ling Z, Guan H, You Z, Wang C, Hu L, Zhang L, Wang Y, Chen S, Xu B and Chen M: Aloperine executes antitumor effects through the induction of apoptosis and cell cycle arrest in prostate cancer in vitro and in vivo. Onco Targets Ther. 11:2735–2743. 2018.PubMed/NCBI View Article : Google Scholar
14	Wang X, Shi J, Niu Z, Wang J and Zhang W: MiR-216a-3p regulates the proliferation, apoptosis, migration, and invasion of lung cancer cells via targeting COPB2. Biosci Biotechnol Biochem: Jul 3, 2020 (Epub ahead of print).
15	Gao ZY, Liu H and Zhang Z: miR-144-3p increases radiosensibility of gastric cancer cells by targeting inhibition of ZEB1. Clin Transl Oncol: Jul 1, 2020 (Epub ahead of print).
16	Chen S, Wang Y, Xu M, Zhang L, Su Y, Wang B and Zhang X: miR-1184 regulates the proliferation and apoptosis of colon cancer cells via targeting CSNK2A1. Mol Cell Probes. 101625:2020.(Epub ahead of print). PubMed/NCBI View Article : Google Scholar
17	Zhou Q, Zhu Y, Wei X, Zhou J, Chang L, Sui H, Han Y, Piao D, Sha R and Bai Y: MiR-590-5p inhibits colorectal cancer angiogenesis and metastasis by regulating nuclear factor 90/vascular endothelial growth factor A axis. Cell Death Dis. 7(e2413)2016.PubMed/NCBI View Article : Google Scholar
18	Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001.PubMed/NCBI View Article : Google Scholar
19	Liu Q, Yang W, Luo Y, Hu S and Zhu L: Correlation between miR-21 and miR-145 and the incidence and prognosis of colorectal cancer. J BUON. 23:29–35. 2018.PubMed/NCBI
20	Hu JL, He GY, Lan XL, Zeng ZC, Guan J, Ding Y, Qian XL, Liao WT, Ding YQ and Liang L: Inhibition of ATG12-mediated autophagy by miR-214 enhances radiosensitivity in colorectal cancer. Oncogenesis. 7(16)2018.PubMed/NCBI View Article : Google Scholar
21	Liao D, Li T, Ye C, Zeng L, Li H, Pu X, Ding C, He Z and Huang GL: miR-221 inhibits autophagy and targets TP53INP1 in colorectal cancer cells. Exp Ther Med. 15:1712–1717. 2018.PubMed/NCBI View Article : Google Scholar
22	Ou C, Sun Z, Li X, Li X, Ren W, Qin Z, Zhang X, Yuan W, Wang J, Yu W, et al: MiR-590-5p, a density-sensitive microRNA, inhibits tumorigenesis by targeting YAP1 in colorectal cancer. Cancer Lett. 399:53–63. 2017.PubMed/NCBI View Article : Google Scholar
23	Wang X, Lai Q, He J, Li Q, Ding J, Lan Z, Gu C, Yan Q, Fang Y, Zhao X and Liu S: LncRNA SNHG6 promotes proliferation, invasion and migration in colorectal cancer cells by activating TGF-β/Smad signaling pathway via targeting UPF1 and inducing EMT via regulation of ZEB1. Int J Med Sci. 16:51–59. 2019.PubMed/NCBI View Article : Google Scholar
24	Peng H, Wang L, Su Q, Yi K, Du J and Wang Z: MiR-31-5p promotes the cell growth, migration and invasion of colorectal cancer cells by targeting NUMB. Biomed Pharmacother. 109:208–216. 2018.PubMed/NCBI View Article : Google Scholar
25	Jiang Z, Cao Q, Dai G, Wang J, Liu C, Lv L and Pan J: Celastrol inhibits colorectal cancer through TGF-beta1/Smad signaling. Onco Targets Ther. 12:509–518. 2019.PubMed/NCBI View Article : Google Scholar
26	Jin Y, Chen W, Yang H, Yan Z, Lai Z, Feng J, Peng J and Lin J: Scutellaria barbata D. Don inhibits migration and invasion of colorectal cancer cells via suppression of PI3K/AKT and TGF-β/Smad signaling pathways. Exp Ther Med. 14:5527–5534. 2017.PubMed/NCBI View Article : Google Scholar
27	Liu Y, Uzair-Ur-Rehman Guo Y, Liang H, Cheng R, Yang F, Hong Y, Zhao C, Liu M, Yu M, et al: miR-181b functions as an oncomiR in colorectal cancer by targeting PDCD4. Protein Cell. 7:722–734. 2016.PubMed/NCBI View Article : Google Scholar
28	Lee YR, Chen SH, Lin CY, Chao WY, Lim YP, Yu HI and Lu CH: In vitro antitumor activity of aloperine on human thyroid cancer cells through caspase-dependent apoptosis. Int J Mol Sci. 19(312)2018.PubMed/NCBI View Article : Google Scholar
29	Wang L, Zhao M, Guo C, Wang G, Zhu F, Wang J, Wang X, Wang Q, Zhao W, Shi Y, et al: PDCD4 deficiency aggravated colitis and Colitis-associated colorectal cancer via promoting IL-6/STAT3 pathway in mice. Inflamm Bowel Dis. 22:1107–1118. 2016.PubMed/NCBI View Article : Google Scholar
30	Lim SC and Hong R: Programmed cell death 4 (Pdcd4) expression in colorectal adenocarcinoma: Association with clinical stage. Oncol Lett. 2:1053–1057. 2011.PubMed/NCBI View Article : Google Scholar