Isoflurane suppresses lung ischemia‑reperfusion injury by inactivating NF‑&kappa;B and inhibiting cell apoptosis

Lv,Ning; Li,Xiaoyun

doi:10.3892/etm.2020.9202

Isoflurane suppresses lung ischemia‑reperfusion injury by inactivating NF‑κB and inhibiting cell apoptosis

Authors:
- Ning Lv
- Xiaoyun Li
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Affiliations: Department of Anesthesiology, Tianjin Central Hospital of Gynecology Obstetrics, Tianjin 300100, P.R. China, Department of Anesthesiology, The Third Affiliated Hospital of Sun Yat‑Sen University, Guangzhou, Guangdong 510630, P.R. China
Published online on: September 10, 2020 https://doi.org/10.3892/etm.2020.9202
Article Number: 74
Copyright: © Lv et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Patients with lung ischemia‑reperfusion injury (LIRI), involving cytokines, including interleukin (IL)‑6 and IL‑8, display poor clinical outcomes. Isoflurane displays protective effects against ischemia‑reperfusion injury in numerous organs. In the present study, the effects of isoflurane on LIRI were investigated in vitro using a hypoxia‑reoxygenation (HR) cell model. The mRNA expression levels of specific genes were analyzed by reverse transcription‑quantitative PCR and protein expression levels were measured by ELISA and western blotting. Cell apoptosis and proliferation were assessed by flow cytometry and the Cell Counting Kit‑8 assay, respectively. Isoflurane pretreatment decreased HR‑induced IL‑6 and IL‑8 expression levels in A549 cells. Isoflurane pretreatment also inhibited HR‑induced cell apoptosis and Bax expression, and reversed HR‑induced downregulation of Bcl‑2 expression. Moreover, isoflurane pretreatment decreased HR‑induced NF‑κB phosphorylated‑p65 protein expression and NF‑κB activation. Furthermore, HR‑induced increases in malondialdehyde concentration and decreases in superoxide dismutase activity were reversed by isoflurane pretreatment. In conclusion, the results indicated that isoflurane suppressed LIRI by inhibiting the activation of NF‑κB and the induction of cell apoptosis.

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