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Isoflurane suppresses lung ischemia‑reperfusion injury by inactivating NF‑κB and inhibiting cell apoptosis

  • Authors:
    • Ning Lv
    • Xiaoyun Li
  • View Affiliations / Copyright

    Affiliations: Department of Anesthesiology, Tianjin Central Hospital of Gynecology Obstetrics, Tianjin 300100, P.R. China, Department of Anesthesiology, The Third Affiliated Hospital of Sun Yat‑Sen University, Guangzhou, Guangdong 510630, P.R. China
    Copyright: © Lv et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 74
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    Published online on: September 10, 2020
       https://doi.org/10.3892/etm.2020.9202
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Abstract

Patients with lung ischemia‑reperfusion injury (LIRI), involving cytokines, including interleukin (IL)‑6 and IL‑8, display poor clinical outcomes. Isoflurane displays protective effects against ischemia‑reperfusion injury in numerous organs. In the present study, the effects of isoflurane on LIRI were investigated in vitro using a hypoxia‑reoxygenation (HR) cell model. The mRNA expression levels of specific genes were analyzed by reverse transcription‑quantitative PCR and protein expression levels were measured by ELISA and western blotting. Cell apoptosis and proliferation were assessed by flow cytometry and the Cell Counting Kit‑8 assay, respectively. Isoflurane pretreatment decreased HR‑induced IL‑6 and IL‑8 expression levels in A549 cells. Isoflurane pretreatment also inhibited HR‑induced cell apoptosis and Bax expression, and reversed HR‑induced downregulation of Bcl‑2 expression. Moreover, isoflurane pretreatment decreased HR‑induced NF‑κB phosphorylated‑p65 protein expression and NF‑κB activation. Furthermore, HR‑induced increases in malondialdehyde concentration and decreases in superoxide dismutase activity were reversed by isoflurane pretreatment. In conclusion, the results indicated that isoflurane suppressed LIRI by inhibiting the activation of NF‑κB and the induction of cell apoptosis.
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1 

Ovechkin AV, Lominadze D, Sedoris KC, Robinson TW, Tyagi SC and Roberts AM: Lung ischemia-reperfusion injury: Implications of oxidative stress and platelet-arteriolar wall interactions. Arch Physiol Biochem. 113:1–12. 2007.PubMed/NCBI View Article : Google Scholar

2 

Den Hengst WA, Gielis JF, Lin JY, Van Schil PE, De Windt LJ and Moens AL: Lung ischemia-reperfusion injury: A molecular and clinical view on a complex pathophysiological process. Am J Physiol Heart Circ Physiol. 299:H1283–H1299. 2010.PubMed/NCBI View Article : Google Scholar

3 

Porteous MK, Diamond JM and Christie JD: Primary graft dysfunction: Lessons learned about the first 72 h after lung transplantation. Curr Opin Organ Transplant. 20:506–514. 2015.PubMed/NCBI View Article : Google Scholar

4 

Kreisel D and Goldstein DR: Innate immunity and organ transplantation: Focus on lung transplantation. Transpl Int. 26:2–10. 2013.PubMed/NCBI View Article : Google Scholar

5 

Ng CS, Wan S, Arifi AA and Yim AP: Inflammatory response to pulmonary ischemia-reperfusion injury. Surg Today. 36:205–214. 2006.PubMed/NCBI View Article : Google Scholar

6 

Welborn MB III, Douglas WG, Abouhamze Z, Auffenburg T, Abouhamze AS, Baumhofer J, Seeger JM, Pruitt JH, Edwards PD, Chizzonite R, et al: Visceral ischemia-reperfusion injury promotes tumor necrosis factor (TNF) and interleukin-1 (IL-1) dependent organ injury in the mouse. Shock. 6:171–176. 1996.PubMed/NCBI

7 

Warltier DC, Al Wathiqui MH, Kampine JP and Schmeling WT: Recovery of contractile function of stunned myocardium in chronically instrumented dogs is enhanced by halothane or isoflurane. Anesthesiology. 69:552–565. 1988.PubMed/NCBI View Article : Google Scholar

8 

Kehl F, Krolikowski JG, Mraovic B, Pagel PS, Warltier DC and Kersten JR: Is isoflurane-induced preconditioning dose related? Anesthesiology. 96:675–680. 2002.PubMed/NCBI View Article : Google Scholar

9 

Lv X, Li Q, Huang SD, Wu FX, Yang LQ and Yu WF: Isoflurane pretreatment reduced liver injury induced by ischemia/reperfusion combined with lipopolysaccharide in rats. Zhongguo Wei Zhong Bing Ji Jiu Yi Xue (Chinese). 20:271–274. 2008.PubMed/NCBI

10 

Liang Y, Li Z, Mo N, Li M, Zhuang Z, Wang J, Wang Y and Guo X: Isoflurane preconditioning ameliorates renal ischemia-reperfusion injury through antiinflammatory and antiapoptotic actions in rats. Biol Pharm Bull. 37:1599–1605. 2014.PubMed/NCBI View Article : Google Scholar

11 

Lv X, Wang ZM, Huang SD, Song SH, Wu FX and Yu WF: Emulsified isoflurane preconditioning reduces lung injury induced by hepatic ischemia/reperfusion in rats. Int J Med Sci. 8:353–361. 2011.PubMed/NCBI View Article : Google Scholar

12 

Lieber M, Smith B, Szakal A, Nelson-Rees W and Todaro G: A continuous tumor-cell line from a human lung carcinoma with properties of type II alveolar epithelial cells. Int J Cancer. 17:62–70. 1976.PubMed/NCBI View Article : Google Scholar

13 

Yue T, Roth Z'graggen B, Blumenthal S, Neff SB, Reyes L, Booy C, Steurer M, Spahn DR, Neff TA, Schmid ER and Beck-Schimmer B: Postconditioning with a volatile anaesthetic in alveolar epithelial cells in vitro. Eur Respir J. 31:118–125. 2008.PubMed/NCBI View Article : Google Scholar

14 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C (T)) method. Methods. 25:402–408. 2001.PubMed/NCBI View Article : Google Scholar

15 

Apostolakis E, Filos KS, Koletsis E and Dougenis D: Lung dysfunction following cardiopulmonary bypass. J Card Surg. 25:47–55. 2010.PubMed/NCBI View Article : Google Scholar

16 

Weyker PD, Webb CA, Kiamanesh D and Flynn BC: Lung ischemia reperfusion injury: A bench-to-beside review. Semin Cardiothorac Vasc Anesth. 17:28–43. 2013.PubMed/NCBI View Article : Google Scholar

17 

Apostolakis EE, Koletsis EN, Baikoussis NG, Siminelakis SN and Papadopoulos GS: Strategies to prevent intraoperative lung injury during cardiopulmonary bypass. J Cardiothorac Surg. 5(1)2010.PubMed/NCBI View Article : Google Scholar

18 

Tang PS, Mura M, Seth R and Liu M: Acute lung injury and cell death: How many ways can cells die? Am J Physiol Lung Cell Mol Physiol. 294:L632–L641. 2008.PubMed/NCBI View Article : Google Scholar

19 

Lindsten T, Ross AJ, King A, Zong WX, Rathmell JC, Shiels HA, Ulrich E, Waymire KG, Mahar P, Frauwirth K, et al: The combined functions of proapoptotic Bcl-2 family members bak and bax are essential for normal development of multiple tissues. Mol Cell. 6:1389–1399. 2000.PubMed/NCBI View Article : Google Scholar

20 

Oda E, Ohki R, Murasawa H, Nemoto J, Shibue T, Yamashita T, Tokino T, Taniguchi T and Tanaka N: Noxa, a BH3-only member of the Bcl-2 family and candidate mediator of p53-induced apoptosis. Science. 288:1053–1058. 2000.PubMed/NCBI View Article : Google Scholar

21 

Goodlad RA: Quantification of epithelial cell proliferation, cell dynamics, and cell kinetics in vivo. Wiley Interdiscip Rev Dev Biol 6: 2017.

22 

De Perrot M, Liu M, Wadderll TK and Kashavjee S: Ischemia reperfusion induced lung injury. Am J Respir Crit Care Med. 167:490–511. 2003.PubMed/NCBI View Article : Google Scholar

23 

Till GO, Hatherill JR, Tourtellotte WW, Lutz MJ and Ward PA: Lipid peroxidation and acute lung injury after thermal trauma to skin. Evidence of a role for hydroxyl radical. Am J Pathol. 119:376–384. 1985.PubMed/NCBI

24 

Baradaran Rahimi V, Rakhshandeh H, Raucci F, Buono B, Shirazinia R, Samzadeh Kermani A, Maione F, Mascolo N and Askari VR: Anti-inflammatory and anti-oxidant activity of portulaca oleracea extract on lps-induced rat lung injury. Molecules. 4: pii(E139)2019.PubMed/NCBI View Article : Google Scholar

25 

Forman HJ and Thomas MJ: Oxidant production and bactericidal activity of phagocytes. Annu Rev Physiol. 48:669–680. 1986.PubMed/NCBI View Article : Google Scholar

26 

Watanabe K, Iwahara C, Nakayama H, Iwabuchi K, Matsukawa T, Yokoyama K, Yamaguchi K, Kamiyama Y and Inada E: Sevoflurane suppresses tumour necrosis factor-a-induced inflammatory responses in small airway epithelial cells after anoxia/reoxygenation. Br J Anaesth. 110:637–645. 2013.PubMed/NCBI View Article : Google Scholar

27 

Li QF, Zhu YS, Jiang H, Xu H and Sun Y: Isoflurane preconditioning ame-liorates endotoxin-induced acute lung injury and mortality in rats. Anesth Analg. 109:1591–1597. 2009.PubMed/NCBI View Article : Google Scholar

28 

Hashiguchi H, Morooka H, Miyoshi H, Matsumoto M, Koji T and Sumikaw K: Isoflurane protects renal function against ischemia and reperfusion through inhibition of protein kinases, JNK and ERK. Anesth Analg. 101:1584–1589. 2005.PubMed/NCBI View Article : Google Scholar

29 

Yoshidome H, Kato A, Edwards MJ and Lentsch AB: Interleukin-10 inhibits pulmonary NF-kappaB activation and lung injury induced by hepatic ischemia-reperfusion. Am J Physiol. 277:919–923. 1999.PubMed/NCBI View Article : Google Scholar

30 

Colletti LM, Cortis A, Lukacs N, Kunkel SL, Green M and Strieter RM: Tumor necrosis factor up-regulates intercellular adhesion molecule 1, which is important in the neutrophil-dependent lung and liver injury associated with hepatic ischemia and reperfusion in the rat. Shock. 10:182–191. 1998.PubMed/NCBI View Article : Google Scholar

31 

Zhong C, Zhou Y and Liu H: Nuclear factor kappaB and anesthetic preconditioning during myocardial ischemia-reperfusion. Anesthesiology. 100:540–545. 2004.PubMed/NCBI View Article : Google Scholar

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Copy and paste a formatted citation
Spandidos Publications style
Lv N and Li X: Isoflurane suppresses lung ischemia‑reperfusion injury by inactivating NF‑κB and inhibiting cell apoptosis. Exp Ther Med 20: 74, 2020.
APA
Lv, N., & Li, X. (2020). Isoflurane suppresses lung ischemia‑reperfusion injury by inactivating NF‑κB and inhibiting cell apoptosis. Experimental and Therapeutic Medicine, 20, 74. https://doi.org/10.3892/etm.2020.9202
MLA
Lv, N., Li, X."Isoflurane suppresses lung ischemia‑reperfusion injury by inactivating NF‑κB and inhibiting cell apoptosis". Experimental and Therapeutic Medicine 20.5 (2020): 74.
Chicago
Lv, N., Li, X."Isoflurane suppresses lung ischemia‑reperfusion injury by inactivating NF‑κB and inhibiting cell apoptosis". Experimental and Therapeutic Medicine 20, no. 5 (2020): 74. https://doi.org/10.3892/etm.2020.9202
Copy and paste a formatted citation
x
Spandidos Publications style
Lv N and Li X: Isoflurane suppresses lung ischemia‑reperfusion injury by inactivating NF‑κB and inhibiting cell apoptosis. Exp Ther Med 20: 74, 2020.
APA
Lv, N., & Li, X. (2020). Isoflurane suppresses lung ischemia‑reperfusion injury by inactivating NF‑κB and inhibiting cell apoptosis. Experimental and Therapeutic Medicine, 20, 74. https://doi.org/10.3892/etm.2020.9202
MLA
Lv, N., Li, X."Isoflurane suppresses lung ischemia‑reperfusion injury by inactivating NF‑κB and inhibiting cell apoptosis". Experimental and Therapeutic Medicine 20.5 (2020): 74.
Chicago
Lv, N., Li, X."Isoflurane suppresses lung ischemia‑reperfusion injury by inactivating NF‑κB and inhibiting cell apoptosis". Experimental and Therapeutic Medicine 20, no. 5 (2020): 74. https://doi.org/10.3892/etm.2020.9202
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