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Bone morphogenetic protein 2‑enhanced osteogenic differentiation of stem cell spheres by regulation of Runx2 expression

  • Authors:
    • Sae Kyung Min
    • Minji Kim
    • Jun-Beom Park
  • View Affiliations / Copyright

    Affiliations: Department of Periodontics, College of Medicine, The Catholic University of Korea, Seoul 06591, Republic of Korea, College of Dentistry, Chosun University, Gwangju 61452, Republic of Korea
    Copyright: © Min et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 79
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    Published online on: September 11, 2020
       https://doi.org/10.3892/etm.2020.9206
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Abstract

Bone morphogenetic protein 2 (BMP‑2) is a growth factor that is used to induce osteogenic differentiation in stem cells. The present study assessed the effects of BMP‑2 on stem cell spheroid morphology, viability and osteogenic differentiation. Stem cell spheres were constructed and treated with BMP‑2 at predetermined concentrations (0‑100 ng/ml) using concave microwells. Cell viability was qualitatively and quantitatively analyzed via microscopy and a water‑soluble tetrazolium salt assay kit, respectively. Alkaline phosphatase activity was assessed and an anthraquinone dye for calcium deposit evaluation was performed to determine osteogenic differentiation. The expressions of (runt‑related transcription factor 2) and collagen 1 were also determined via quantitative PCR. Spherical shapes were formed using concave microwells on day 1, which were maintained up to day 21. On day 1, the relative cell viability of 0, 10 and 100 ng/ml BMP‑2 treated cells was 100.0±1.9, 97.3±4.4 and 101.3±2.6%, respectively. Significantly higher values for alkaline phosphatase activity were determined in the 100 ng/ml treated group when compared with the control group. Additionally, Runx2 mRNA levels were significantly higher in the 100 ng/ml BMP‑2 group compared with the control group, as determined via quantitative PCR. The results of the present study indicated that BMP‑2 enhanced the differentiation of stem cell spheres, which was demonstrated by increased alkaline phosphatase activity and Runx2 expression.
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Copy and paste a formatted citation
Spandidos Publications style
Min S, Kim M and Park J: Bone morphogenetic protein 2‑enhanced osteogenic differentiation of stem cell spheres by regulation of Runx2 expression. Exp Ther Med 20: 79, 2020.
APA
Min, S., Kim, M., & Park, J. (2020). Bone morphogenetic protein 2‑enhanced osteogenic differentiation of stem cell spheres by regulation of Runx2 expression. Experimental and Therapeutic Medicine, 20, 79. https://doi.org/10.3892/etm.2020.9206
MLA
Min, S., Kim, M., Park, J."Bone morphogenetic protein 2‑enhanced osteogenic differentiation of stem cell spheres by regulation of Runx2 expression". Experimental and Therapeutic Medicine 20.5 (2020): 79.
Chicago
Min, S., Kim, M., Park, J."Bone morphogenetic protein 2‑enhanced osteogenic differentiation of stem cell spheres by regulation of Runx2 expression". Experimental and Therapeutic Medicine 20, no. 5 (2020): 79. https://doi.org/10.3892/etm.2020.9206
Copy and paste a formatted citation
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Spandidos Publications style
Min S, Kim M and Park J: Bone morphogenetic protein 2‑enhanced osteogenic differentiation of stem cell spheres by regulation of Runx2 expression. Exp Ther Med 20: 79, 2020.
APA
Min, S., Kim, M., & Park, J. (2020). Bone morphogenetic protein 2‑enhanced osteogenic differentiation of stem cell spheres by regulation of Runx2 expression. Experimental and Therapeutic Medicine, 20, 79. https://doi.org/10.3892/etm.2020.9206
MLA
Min, S., Kim, M., Park, J."Bone morphogenetic protein 2‑enhanced osteogenic differentiation of stem cell spheres by regulation of Runx2 expression". Experimental and Therapeutic Medicine 20.5 (2020): 79.
Chicago
Min, S., Kim, M., Park, J."Bone morphogenetic protein 2‑enhanced osteogenic differentiation of stem cell spheres by regulation of Runx2 expression". Experimental and Therapeutic Medicine 20, no. 5 (2020): 79. https://doi.org/10.3892/etm.2020.9206
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