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The combination of PLLA/PLGA/PCL composite scaffolds integrated with BMP‑2‑loaded microspheres and low‑intensity pulsed ultrasound alleviates steroid‑induced osteonecrosis of the femoral head

  • Authors:
    • Hanxiao Zhu
    • Zhongli Shi
    • Xunzi Cai
    • Xiaobo Yang
    • Chenhe Zhou
  • View Affiliations / Copyright

    Affiliations: Department of Orthopaedic Surgery, The Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou, Zhejiang 310009, P.R. China
    Copyright: © Zhu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 126
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    Published online on: September 24, 2020
       https://doi.org/10.3892/etm.2020.9254
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Abstract

Low‑intensity pulsed ultrasound (LIPUS), which has been previously reported to promote bone repair, is proposed to be a noninvasive form of therapy for the treatment of osteonecrosis. Bone fillers made from composite scaffolds have been demonstrated to be effective for preventing bone defects such as osteonecrosis. The present study aimed to investigate whether the application of LIPUS combined with bone morphogenetic protein‑2 (BMP‑2)‑loaded poly‑L‑lactic acid/polylactic‑co‑glycolic acid/poly‑ε‑caprolactone (PLLA/PLGA/PCL) composite scaffolds can improve recovery in a rat model of steroid‑induced osteonecrosis of the femoral head (ONFH). BMP‑2‑loaded PLGA microspheres incorporated into PLLA/PLGA/PCL composite scaffolds were constructed. Bilateral femoral head LIPUS intervention was conducted in rats with steroid‑induced ONFH. LIPUS intervention alone contributed to the alleviation of osteonecrosis, in addition to improving load‑carrying capacity and accelerated bone formation, angiogenesis and differentiation. Subsequently, femoral head parameters and assessment of load‑carrying capacity, bone formation‑related factors, and angiogenesis‑ and differentiation‑related factors were measured in rats with or without implanted BMP‑2‑loaded PLLA/PLGA/PCL composite scaffolds. LIPUS combined with the implantation of PLLA/PLGA/PCL composite scaffolds loaded with BMP‑2 microspheres protected rats against steroid‑induced ONFH and improved load‑carrying capacity, bone formation, angiogenesis and differentiation. Together, these data support the use of BMP‑2‑loaded PLLA/PLGA/PCL composite scaffolds combined with LIPUS for ONFH as a potential alternative curative solution for treating bone diseases.
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1 

Tripathy SK, Goyal T and Sen RK: Management of femoral head osteonecrosis: Current concepts. Indian J Orthop. 49:28–45. 2015.PubMed/NCBI View Article : Google Scholar

2 

Issa K, Pivec R, Kapadia BH, Banerjee S and Mont MA: Osteonecrosis of the femoral head: The total hip replacement solution. Bone Joint J. 95-B (11 Suppl A):S46–S50. 2013.PubMed/NCBI View Article : Google Scholar

3 

Mont MA, Cherian JJ, Sierra RJ, Jones LC and Lieberman JR: Nontraumatic osteonecrosis of the femoral head: Where do we stand today? A ten-year update. J Bone Joint Surg Am. 97:1604–1627. 2015.PubMed/NCBI View Article : Google Scholar

4 

Zalavras CG and Lieberman JR: Osteonecrosis of the femoral head: Evaluation and treatment. J Am Acad Orthop Surg. 22:455–464. 2014.PubMed/NCBI View Article : Google Scholar

5 

Powell C, Chang C, Naguwa SM, Cheema G and Gershwin ME: Steroid induced osteonecrosis: An analysis of steroid dosing risk. Autoimmun Rev. 9:721–743. 2010.PubMed/NCBI View Article : Google Scholar

6 

Powell C, Chang C and Gershwin ME: Current concepts on the pathogenesis and natural history of steroid-induced osteonecrosis. Clin Rev Allergy Immunol. 41:102–113. 2011.PubMed/NCBI View Article : Google Scholar

7 

Yan SG, Huang LY and Cai XZ: Low-intensity pulsed ultrasound: A potential non-invasive therapy for femoral head osteonecrosis. Med Hypotheses. 76:4–7. 2011.PubMed/NCBI View Article : Google Scholar

8 

Atesok K, Fu FH, Wolf MR, Ochi M, Jazrawi LM, Doral MN, Lubowitz JH and Rodeo SA: Augmentation of tendon-to-bone healing. J Bone Joint Surg Am. 96:513–521. 2014.PubMed/NCBI View Article : Google Scholar

9 

Agarwal R and Garcia AJ: Biomaterial strategies for engineering implants for enhanced osseointegration and bone repair. Adv Drug Deliv Rev. 94:53–62. 2015.PubMed/NCBI View Article : Google Scholar

10 

Balmayor ER: Targeted delivery as key for the success of small osteoinductive molecules. Adv Drug Deliv Rev. 94:13–27. 2015.PubMed/NCBI View Article : Google Scholar

11 

Salazar VS, Gamer LW and Rosen V: BMP signalling in skeletal development, disease and repair. Nat Rev Endocrinol. 12:203–221. 2016.PubMed/NCBI View Article : Google Scholar

12 

Wang C, Zang H and Zhou D: Bone morphogenetic protein-2 exhibits therapeutic benefits for osteonecrosis of the femoral head through induction of cartilage and bone cells. Exp Ther Med. 15:4298–4308. 2018.PubMed/NCBI View Article : Google Scholar

13 

Sun W, Li Z, Gao F, Shi Z, Zhang Q and Guo W: Recombinant human bone morphogenetic protein-2 in debridement and impacted bone graft for the treatment of femoral head osteonecrosis. PLoS One. 9(e100424)2014.PubMed/NCBI View Article : Google Scholar

14 

Lo KW, Ulery BD, Ashe KM and Laurencin CT: Studies of bone morphogenetic protein-based surgical repair. Adv Drug Deliv Rev. 64:1277–1291. 2012.PubMed/NCBI View Article : Google Scholar

15 

King WJ and Krebsbach PH: Growth factor delivery: How surface interactions modulate release in vitro and in vivo. Adv Drug Deliv Rev. 64:1239–1256. 2012.PubMed/NCBI View Article : Google Scholar

16 

Fitzgerald R, Bass LM, Goldberg DJ, Graivier MH and Lorenc ZP: Physiochemical characteristics of poly-L-lactic acid (PLLA). Aesthet Surg J. 38 (Suppl 1):S13–S17. 2018.PubMed/NCBI View Article : Google Scholar

17 

Martins AF, Facchi SP, da Camara PCF, Camargo SEA, Camargo CHR, Popat KC and Kipper MJ: Novel poly(ε-caprolactone)/amino-functionalized tannin electrospun membranes as scaffolds for tissue engineering. J Colloid Interface Sci. 525:21–30. 2018.PubMed/NCBI View Article : Google Scholar

18 

Barthold SW, Bayne KA, Davis MA, Bayne K and Davis M: Guide for the care and use of laboratory animals. Publication no. 85-23(rev.). 327:963–965. 2011.PubMed/NCBI View Article : Google Scholar

19 

Mercado AE, Ma J, He X and Jabbari E: Release characteristics and osteogenic activity of recombinant human bone morphogenetic protein-2 grafted to novel self-assembled poly(lactide-co-glycolide fumarate) nanoparticles. J Control Release. 140:148–156. 2009.PubMed/NCBI View Article : Google Scholar

20 

Drescher W, Bünger MH, Weigert K, Bünger C and Hansen ES: Methylprednisolone enhances contraction of porcine femoral head epiphyseal arteries. Clin Orthop Relat Res. 112–117. 2004.PubMed/NCBI View Article : Google Scholar

21 

Dong Y, Li Y, Huang C, Gao K and Weng X: Systemic application of teriparatide for steroid induced osteonecrosis in a rat model. BMC Musculoskelet Disord. 16(163)2015.PubMed/NCBI View Article : Google Scholar

22 

Mcpherson C: Regulation of animal care and research? NIH's opinion. J Anim Sci. 51:492–496. 1980.PubMed/NCBI View Article : Google Scholar

23 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001.PubMed/NCBI View Article : Google Scholar

24 

Florencio-Silva R, Sasso GR, Sasso-Cerri E, Simões MJ and Cerri PS: Biology of bone tissue: Structure, function, and factors that influence bone cells. Biomed Res Int. 2015(421746)2015.PubMed/NCBI View Article : Google Scholar

25 

Tian H, Du J, Wen J, Liu Y, Montgomery SR, Scott TP, Aghdasi B, Xiong C, Suzuki A, Hayashi T, et al: Growth-factor nanocapsules that enable tunable controlled release for bone regeneration. ACS Nano. 10:7362–7369. 2016.PubMed/NCBI View Article : Google Scholar

26 

Qin L, Yao D, Zheng L, Liu WC, Liu Z, Lei M, Huang L, Xie X, Wang X, Chen Y, et al: Phytomolecule icaritin incorporated PLGA/TCP scaffold for steroid-associated osteonecrosis: Proof-of-concept for prevention of hip joint collapse in bipedal emus and mechanistic study in quadrupedal rabbits. Biomaterials. 59:125–143. 2015.PubMed/NCBI View Article : Google Scholar

27 

Zheng Z, Yin W, Zara JN, Li W, Kwak J, Mamidi R, Lee M, Siu RK, Ngo R, Wang J, et al: The use of BMP-2 coupled-nanosilver-PLGA composite grafts to induce bone repair in grossly infected segmental defects. Biomaterials. 31:9293–9300. 2010.PubMed/NCBI View Article : Google Scholar

28 

Yilgor P, Yilmaz G, Onal MB, Solmaz I, Gundogdu S, Keskil S, Sousa RA, Reis RL, Hasirci N and Hasirci V: An in vivo study on the effect of scaffold geometry and growth factor release on the healing of bone defects. J Tissue Eng Regen Med. 7:687–696. 2013.PubMed/NCBI View Article : Google Scholar

29 

Zhang H, Migneco F, Lin CY and Hollister SJ: Chemically-conjugated bone morphogenetic protein-2 on three-dimensional polycaprolactone scaffolds stimulates osteogenic activity in bone marrow stromal cells. Tissue Eng Part A. 16:3441–3448. 2010.PubMed/NCBI View Article : Google Scholar

30 

Huang KC, Yano F, Murahashi Y, Takano S, Kitaura Y, Chang SH, Soma K, Ueng SWN, Tanaka S, Ishihara K, et al: Sandwich-type PLLA-nanosheets loaded with BMP-2 induce bone regeneration in critical-sized mouse calvarial defects. Acta Biomater. 59:12–20. 2017.PubMed/NCBI View Article : Google Scholar

31 

Zhu H, Cai X, Lin T, Shi Z and Yan S: Low-intensity pulsed ultrasound enhances bone repair in a rabbit model of steroid-associated osteonecrosis. Clin Orthop Relat Res. 473:1830–1839. 2015.PubMed/NCBI View Article : Google Scholar

32 

Gleizal A, Ferreira S, Lavandier B, Simon B, Béziat JL and Béra JC: The impact of low intensity pulsed ultrasound on mouse skull bone osteoblast cultures. Rev Stomatol Chir Maxillofac. 111:280–285. 2010.PubMed/NCBI View Article : Google Scholar : (In French).

33 

Zhou XY, Wu SY, Zhang ZC, Wang F, Yang YL, Li M and Wei XZ: Low-intensity pulsed ultrasound promotes endothelial cell-mediated osteogenesis in a conditioned medium coculture system with osteoblasts. Medicine (Baltimore). 96(e8397)2017.PubMed/NCBI View Article : Google Scholar

34 

Tsai KS, Kao SY, Wang CY, Wang YJ, Wang JP and Hung SC: Type I collagen promotes proliferation and osteogenesis of human mesenchymal stem cells via activation of ERK and Akt pathways. J Biomed Mater Res A. 94:673–682. 2010.PubMed/NCBI View Article : Google Scholar

35 

Li J, Fan L, Yu Z, Dang X and Wang K: The effect of deferoxamine on angiogenesis and bone repair in steroid-induced osteonecrosis of rabbit femoral heads. Exp Biol Med (Maywood). 240:273–280. 2015.PubMed/NCBI View Article : Google Scholar

36 

Suzuki A, Takayama T, Suzuki N, Kojima T, Ota N, Asano S and Ito K: Daily low-intensity pulsed ultrasound stimulates production of bone morphogenetic protein in ROS 17/2.8 cells. J Oral Sci. 51:29–36. 2009.PubMed/NCBI View Article : Google Scholar

37 

Wijdicks CA, Virdi AS, Sena K, Sumner DR and Leven RM: Ultrasound enhances recombinant human BMP-2 induced ectopic bone formation in a rat model. Ultrasound Med Biol. 35:1629–1637. 2009.PubMed/NCBI View Article : Google Scholar

38 

Cheung WH, Chow SK, Sun MH, Qin L and Leung KS: Low-intensity pulsed ultrasound accelerated callus formation, angiogenesis and callus remodeling in osteoporotic fracture healing. Ultrasound Med Biol. 37:231–238. 2011.PubMed/NCBI View Article : Google Scholar

39 

Shindo T, Ito K, Ogata T, Hatanaka K, Kurosawa R, Eguchi K, Kagaya Y, Hanawa K, Aizawa K, Shiroto T, et al: Low-lntensity pulsed ultrasound enhances angiogenesis and ameliorates left ventricular dysfunction in a mouse model of acute myocardial infarction. Arterioscler Thromb Vasc Biol. 36:1220–1229. 2016.PubMed/NCBI View Article : Google Scholar

40 

Chen WC, Chung CH, Lu YC, Wu MH, Chou PH, Yen JY, Lai YW, Wang GS, Liu SC, Cheng JK, et al: BMP-2 induces angiogenesis by provoking integrin α6 expression in human endothelial progenitor cells. Biochem Pharmacol. 150:256–266. 2018.PubMed/NCBI View Article : Google Scholar

41 

Lv J, Xiu P, Tan J, Jia Z, Cai H and Liu Z: Enhanced angiogenesis and osteogenesis in critical bone defects by the controlled release of BMP-2 and VEGF: Implantation of electron beam melting-fabricated porous Ti6Al4V scaffolds incorporating growth factor-doped fibrin glue. Biomed Mater. 10(035013)2015.PubMed/NCBI View Article : Google Scholar

42 

Weivoda MM, Ruan M, Pederson L, Hachfeld C, Davey RA, Zajac JD, Westendorf JJ, Khosla S and Oursler MJ: Osteoclast TGF-β receptor signaling induces Wnt1 secretion and couples bone resorption to bone formation. J Bone Miner Res. 31:76–85. 2016.PubMed/NCBI View Article : Google Scholar

43 

Fujiwara M and Ozono K: Cytokines and osteogenesis. Clin Calcium. 24:845–851. 2014.PubMed/NCBI(In Japanese).

44 

Liu DD, Zhang JC, Zhang Q, Wang SX and Yang MS: TGF-β/BMP signaling pathway is involved in cerium-promoted osteogenic differentiation of mesenchymal stem cells. J Cell Biochem. 114:1105–1114. 2013.PubMed/NCBI View Article : Google Scholar

45 

Chen G, Deng C and Li YP: TGF-β and BMP signaling in osteoblast differentiation and bone formation. Int J Biol Sci. 8:272–288. 2012.PubMed/NCBI View Article : Google Scholar

46 

Li XL, Liu YB, Ma EG, Shen WX, Li H and Zhang YN: Synergistic effect of BMP9 and TGF-β in the proliferation and differentiation of osteoblasts. Genet Mol Res. 14:7605–7615. 2015.PubMed/NCBI View Article : Google Scholar

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Copy and paste a formatted citation
Spandidos Publications style
Zhu H, Shi Z, Cai X, Yang X and Zhou C: The combination of PLLA/PLGA/PCL composite scaffolds integrated with BMP‑2‑loaded microspheres and low‑intensity pulsed ultrasound alleviates steroid‑induced osteonecrosis of the femoral head. Exp Ther Med 20: 126, 2020.
APA
Zhu, H., Shi, Z., Cai, X., Yang, X., & Zhou, C. (2020). The combination of PLLA/PLGA/PCL composite scaffolds integrated with BMP‑2‑loaded microspheres and low‑intensity pulsed ultrasound alleviates steroid‑induced osteonecrosis of the femoral head. Experimental and Therapeutic Medicine, 20, 126. https://doi.org/10.3892/etm.2020.9254
MLA
Zhu, H., Shi, Z., Cai, X., Yang, X., Zhou, C."The combination of PLLA/PLGA/PCL composite scaffolds integrated with BMP‑2‑loaded microspheres and low‑intensity pulsed ultrasound alleviates steroid‑induced osteonecrosis of the femoral head". Experimental and Therapeutic Medicine 20.6 (2020): 126.
Chicago
Zhu, H., Shi, Z., Cai, X., Yang, X., Zhou, C."The combination of PLLA/PLGA/PCL composite scaffolds integrated with BMP‑2‑loaded microspheres and low‑intensity pulsed ultrasound alleviates steroid‑induced osteonecrosis of the femoral head". Experimental and Therapeutic Medicine 20, no. 6 (2020): 126. https://doi.org/10.3892/etm.2020.9254
Copy and paste a formatted citation
x
Spandidos Publications style
Zhu H, Shi Z, Cai X, Yang X and Zhou C: The combination of PLLA/PLGA/PCL composite scaffolds integrated with BMP‑2‑loaded microspheres and low‑intensity pulsed ultrasound alleviates steroid‑induced osteonecrosis of the femoral head. Exp Ther Med 20: 126, 2020.
APA
Zhu, H., Shi, Z., Cai, X., Yang, X., & Zhou, C. (2020). The combination of PLLA/PLGA/PCL composite scaffolds integrated with BMP‑2‑loaded microspheres and low‑intensity pulsed ultrasound alleviates steroid‑induced osteonecrosis of the femoral head. Experimental and Therapeutic Medicine, 20, 126. https://doi.org/10.3892/etm.2020.9254
MLA
Zhu, H., Shi, Z., Cai, X., Yang, X., Zhou, C."The combination of PLLA/PLGA/PCL composite scaffolds integrated with BMP‑2‑loaded microspheres and low‑intensity pulsed ultrasound alleviates steroid‑induced osteonecrosis of the femoral head". Experimental and Therapeutic Medicine 20.6 (2020): 126.
Chicago
Zhu, H., Shi, Z., Cai, X., Yang, X., Zhou, C."The combination of PLLA/PLGA/PCL composite scaffolds integrated with BMP‑2‑loaded microspheres and low‑intensity pulsed ultrasound alleviates steroid‑induced osteonecrosis of the femoral head". Experimental and Therapeutic Medicine 20, no. 6 (2020): 126. https://doi.org/10.3892/etm.2020.9254
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