Modulation of SERCA2a expression and function by ultrasound‑guided myocardial gene transfection

Gou,Zhongshan; Yan,Xinxin; Jia,Hongjing; Sun,Kangyun; Li,Ping; Zhang,Qian; Deng,Xuedong

doi:10.3892/etm.2020.9261

Modulation of SERCA2a expression and function by ultrasound‑guided myocardial gene transfection

Authors:
- Zhongshan Gou
- Xinxin Yan
- Hongjing Jia
- Kangyun Sun
- Ping Li
- Qian Zhang
- Xuedong Deng
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Affiliations: Center for Medical Ultrasound, The Affiliated Suzhou Hospital of Nanjing Medical University, Suzhou, Jiangsu 215000, P.R. China, Department of Pharmacy, The Affiliated Suzhou Hospital of Nanjing Medical University, Suzhou, Jiangsu 215000, P.R. China, Department of Cardiology, The Affiliated Suzhou Hospital of Nanjing Medical University, Suzhou, Jiangsu 215000, P.R. China
Published online on: October 2, 2020 https://doi.org/10.3892/etm.2020.9261
Article Number: 132
Copyright: © Gou et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Sarco/endoplasmic reticulum Ca^²+‑ATPase (SERCA2a) is important for cardiac physiological function and pathological progression. However, intravenous injection, a commonly applied approach for gene delivery in most studies investigating the expression of SERCA2a in cardiomyocytes, has not been particularly satisfactory. Therefore, in the present study, a modified method was used to transfect this gene into the heart. Specifically, a SERCA2a‑knockdown lentivirus was directly injected into the myocardium of adult rats under ultrasound guidance, following which the effectiveness and feasibility of this proposed approach were evaluated. The results demonstrated that compared with traditional intravenous injection, the modified gene delivery method resulted in markedly higher transfection efficiency. In addition, the SERCA2a‑knockdown rats exhibited higher rates of arrhythmia and weaker ventricular wall motions compared with those in the control rats, with these symptoms more evident in the rats that received a direct injection into the myocardium compared with those that were intravenously injected. These results suggest that ultrasound‑guided injection into the myocardium is an efficient and safe method for gene delivery and for inducing the knockdown of SERCA2a protein expression in cardiomyocytes in their native environment.

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