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Sarpogrelate and rosuvastatin synergistically ameliorate aortic damage induced by hyperlipidemia in apolipoprotein E‑deficient mice

  • Authors:
    • Hongyang Liu
    • Siwei Xu
    • Guihua Li
    • Dayuan Lou
    • Xiaodan Fu
    • Qin Lu
    • Liman Hao
    • Jingsi Zhang
    • Jiajie Mei
    • Zheng Sui
    • Yu Lou
  • View Affiliations / Copyright

    Affiliations: Department of Heart Intensive Care Unit, The First Affiliated Hospital of Dalian Medical University, Dalian, Liaoning 116001, P.R. China, Department of Cardiology, Affiliated Zhongshan Hospital of Dalian University, Dalian, Liaoning 116001, P.R. China, Department of Cardiology, The Second Affiliated Hospital of Dalian Medical University, Dalian, Liaoning 116023, P.R. China, Department of Cardiology, Jiche Hospital of Dalian, Dalian, Liaoning 116021, P.R. China
    Copyright: © Liu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 170
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    Published online on: October 9, 2020
       https://doi.org/10.3892/etm.2020.9300
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Abstract

The current study aimed to investigate whether sarpogrelate and rosuvastatin possess anti‑arterial injury, and attempted to elucidate the mechanism of action underlying this activity. Sarpogrelate, a 5‑hydroxytryptamine type 2A antagonist, is extensively used to prevent arterial thrombosis; however, its effects on atherosclerosis remain unknown. In the present study, sarpogrelate combined with rosuvastatin or rosuvastatin alone were administered to male ApoE‑/‑ mice fed a high‑fat diet (HFD) for 8 weeks. Metabolic parameters in the blood samples were analyzed using an automatic analyzer. Aortic tissues were stained with hematoxylin and eosin for morphological analysis. The expression levels of oxidized‑low density lipoprotein (LDL) specific scavenging receptors, lectin‑like oxidized low‑density lipoprotein receptor‑1 (LOX‑1) and cluster of differentiation 68 were detected via immunostaining. mRNA expression levels of interleukin (IL)‑1β, IL‑6 and tumor necrosis factor‑α were determined via reverse transcription‑quantitative PCR analysis, while protein expression levels of LOX‑1 and phosphor(p)‑ERK were determined via western blot analysis. The results demonstrated that sarpogrelate combined with rosuvastatin treatment significantly decreased total cholesterol and LDL cholesterol levels in the serum, and alleviated intimal hyperplasia and lipid deposition, accompanied by decreased inflammatory cell infiltration and lower expression levels of inflammatory cytokines, compared with rosuvastatin monotherapy or HFD treatment. Furthermore, sarpogrelate combined with rosuvastatin treatment significantly decreased the expression levels of LOX‑1 and p‑ERK. Taken together, these results suggest that the positive effects of sarpogrelate combined with rosuvastatin treatment on aortic injury may be associated with the regulation of the LOX‑1/p‑ERK signaling pathway. Sarpogrelate and rosuvastatin synergistically decreased aortic damage in ApoE‑/‑ HFD mice, and thus provide a basis for the treatment of aortic injury caused by hyperlipidemia with sarpogrelate.
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Copy and paste a formatted citation
Spandidos Publications style
Liu H, Xu S, Li G, Lou D, Fu X, Lu Q, Hao L, Zhang J, Mei J, Sui Z, Sui Z, et al: Sarpogrelate and rosuvastatin synergistically ameliorate aortic damage induced by hyperlipidemia in apolipoprotein E‑deficient mice. Exp Ther Med 20: 170, 2020.
APA
Liu, H., Xu, S., Li, G., Lou, D., Fu, X., Lu, Q. ... Lou, Y. (2020). Sarpogrelate and rosuvastatin synergistically ameliorate aortic damage induced by hyperlipidemia in apolipoprotein E‑deficient mice. Experimental and Therapeutic Medicine, 20, 170. https://doi.org/10.3892/etm.2020.9300
MLA
Liu, H., Xu, S., Li, G., Lou, D., Fu, X., Lu, Q., Hao, L., Zhang, J., Mei, J., Sui, Z., Lou, Y."Sarpogrelate and rosuvastatin synergistically ameliorate aortic damage induced by hyperlipidemia in apolipoprotein E‑deficient mice". Experimental and Therapeutic Medicine 20.6 (2020): 170.
Chicago
Liu, H., Xu, S., Li, G., Lou, D., Fu, X., Lu, Q., Hao, L., Zhang, J., Mei, J., Sui, Z., Lou, Y."Sarpogrelate and rosuvastatin synergistically ameliorate aortic damage induced by hyperlipidemia in apolipoprotein E‑deficient mice". Experimental and Therapeutic Medicine 20, no. 6 (2020): 170. https://doi.org/10.3892/etm.2020.9300
Copy and paste a formatted citation
x
Spandidos Publications style
Liu H, Xu S, Li G, Lou D, Fu X, Lu Q, Hao L, Zhang J, Mei J, Sui Z, Sui Z, et al: Sarpogrelate and rosuvastatin synergistically ameliorate aortic damage induced by hyperlipidemia in apolipoprotein E‑deficient mice. Exp Ther Med 20: 170, 2020.
APA
Liu, H., Xu, S., Li, G., Lou, D., Fu, X., Lu, Q. ... Lou, Y. (2020). Sarpogrelate and rosuvastatin synergistically ameliorate aortic damage induced by hyperlipidemia in apolipoprotein E‑deficient mice. Experimental and Therapeutic Medicine, 20, 170. https://doi.org/10.3892/etm.2020.9300
MLA
Liu, H., Xu, S., Li, G., Lou, D., Fu, X., Lu, Q., Hao, L., Zhang, J., Mei, J., Sui, Z., Lou, Y."Sarpogrelate and rosuvastatin synergistically ameliorate aortic damage induced by hyperlipidemia in apolipoprotein E‑deficient mice". Experimental and Therapeutic Medicine 20.6 (2020): 170.
Chicago
Liu, H., Xu, S., Li, G., Lou, D., Fu, X., Lu, Q., Hao, L., Zhang, J., Mei, J., Sui, Z., Lou, Y."Sarpogrelate and rosuvastatin synergistically ameliorate aortic damage induced by hyperlipidemia in apolipoprotein E‑deficient mice". Experimental and Therapeutic Medicine 20, no. 6 (2020): 170. https://doi.org/10.3892/etm.2020.9300
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