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Article Open Access

MicroRNA‑124 regulates cell pyroptosis during cerebral ischemia‑reperfusion injury by regulating STAT3

  • Authors:
    • Hui Sun
    • Jing-Jing Li
    • Zi-Ren Feng
    • Hai-Ying Liu
    • Ai-Guo Meng
  • View Affiliations / Copyright

    Affiliations: Department of Clinical Laboratory, Affiliated Hospital of North China University of Science and Technology, Tangshan, Hebei 063000, P.R. China
    Copyright: © Sun et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 227
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    Published online on: October 15, 2020
       https://doi.org/10.3892/etm.2020.9357
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Abstract

Cerebral ischemia‑reperfusion injury (CIRI) is the observed continuation and deterioration of ischemic injury, and currently, there are no effective treatment strategies for the condition. It has been reported that microRNAs (miRNAs) serve an important role in CIRI by regulating pyroptosis. The present study demonstrated that miRNA‑124 regulated CIRI by regulating STAT3. To explore the relationship between miRNA‑124/STAT3 and pyroptosis in CIRI, CIRI was simulated using a middle cerebral artery occlusion model. Subsequently, miRNA‑124 expression levels were altered via the intracerebroventricular injection of miRNA‑124 agonist or antagonist. The degree of brain tissue injury was assessed by conducting TTC staining and neurological function scoring. Relative miRNA‑124 expression levels were determined via reverse transcription‑quantitative PCR. A luciferase reporter gene system verified the targeted binding of miRNA‑124 to STAT3. The expression levels of key proteins and proinflammatory cytokines associated with pyroptosis [caspase‑1, gasdermin D, interleukin (IL)‑18 and IL‑1β] were detected via western blotting and immunohistochemistry. The increased expression levels of pyroptosis‑associated proteins and proinflammatory cytokines in the I/R groups compared with the control group, indicated that pyroptosis intensified over time during CIRI, and miRNA‑124 agonist significantly abrogated pyroptosis and improved neurological function compared with the control group. Furthermore, miRNA‑124 inhibited STAT3 activation in a targeted manner, which also decreased the extent of pyroptosis. However, miRNA‑124 antagonist reversed miR‑124 agonist‑mediated effects. Therefore, the present study indicated that miRNA‑124 may provide neuroprotection against pyroptosis during CIRI, potentially via inhibition of the STAT3 signaling pathway.
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Copy and paste a formatted citation
Spandidos Publications style
Sun H, Li J, Feng Z, Liu H and Meng A: MicroRNA‑124 regulates cell pyroptosis during cerebral ischemia‑reperfusion injury by regulating STAT3. Exp Ther Med 20: 227, 2020.
APA
Sun, H., Li, J., Feng, Z., Liu, H., & Meng, A. (2020). MicroRNA‑124 regulates cell pyroptosis during cerebral ischemia‑reperfusion injury by regulating STAT3. Experimental and Therapeutic Medicine, 20, 227. https://doi.org/10.3892/etm.2020.9357
MLA
Sun, H., Li, J., Feng, Z., Liu, H., Meng, A."MicroRNA‑124 regulates cell pyroptosis during cerebral ischemia‑reperfusion injury by regulating STAT3". Experimental and Therapeutic Medicine 20.6 (2020): 227.
Chicago
Sun, H., Li, J., Feng, Z., Liu, H., Meng, A."MicroRNA‑124 regulates cell pyroptosis during cerebral ischemia‑reperfusion injury by regulating STAT3". Experimental and Therapeutic Medicine 20, no. 6 (2020): 227. https://doi.org/10.3892/etm.2020.9357
Copy and paste a formatted citation
x
Spandidos Publications style
Sun H, Li J, Feng Z, Liu H and Meng A: MicroRNA‑124 regulates cell pyroptosis during cerebral ischemia‑reperfusion injury by regulating STAT3. Exp Ther Med 20: 227, 2020.
APA
Sun, H., Li, J., Feng, Z., Liu, H., & Meng, A. (2020). MicroRNA‑124 regulates cell pyroptosis during cerebral ischemia‑reperfusion injury by regulating STAT3. Experimental and Therapeutic Medicine, 20, 227. https://doi.org/10.3892/etm.2020.9357
MLA
Sun, H., Li, J., Feng, Z., Liu, H., Meng, A."MicroRNA‑124 regulates cell pyroptosis during cerebral ischemia‑reperfusion injury by regulating STAT3". Experimental and Therapeutic Medicine 20.6 (2020): 227.
Chicago
Sun, H., Li, J., Feng, Z., Liu, H., Meng, A."MicroRNA‑124 regulates cell pyroptosis during cerebral ischemia‑reperfusion injury by regulating STAT3". Experimental and Therapeutic Medicine 20, no. 6 (2020): 227. https://doi.org/10.3892/etm.2020.9357
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