Expression profiles of the Wnt/&beta;‑catenin signaling‑related extracellular antagonists during proliferation and differentiation in human osteoblast‑like cells

Parra‑Torres,Alma Y.; Enríquez,Juana; Jiménez‑Ortega,Rogelio F.; Patiño,Nelly; Castillejos‑López,Manuel De Jesús; Torres‑Espíndola,Luz M.; Ramírez‑Salazar,Eric G.; Velázquez‑Cruz,Rafael

doi:10.3892/etm.2020.9384

Expression profiles of the Wnt/β‑catenin signaling‑related extracellular antagonists during proliferation and differentiation in human osteoblast‑like cells

Authors:
- Alma Y. Parra‑Torres
- Juana Enríquez
- Rogelio F. Jiménez‑Ortega
- Nelly Patiño
- Manuel De Jesús Castillejos‑López
- Luz M. Torres‑Espíndola
- Eric G. Ramírez‑Salazar
- Rafael Velázquez‑Cruz
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Affiliations: Genomics of Bone Metabolism Laboratory, National Institute of Genomic Medicine (INMEGEN), Mexico City 14610, Mexico, Department of Reproduction Biology Carlos Gual Castro, National Institute of Medical Sciences and Nutrition Salvador Zubirán (INCMNSZ), Mexico City 14080, Mexico, Subdirection of Clinical Applications Development, National Institute of Genomic Medicine (INMEGEN), Mexico City 14610, Mexico, Epidemiological Surveillance Unit, National Institute of Respiratory Diseases (INER) ‘Ismael Cosío Villegas’, Mexico City 14080, Mexico, Pharmacology Laboratory, National Institute of Pediatrics (INP), Mexico City 04530, Mexico, National Council for Science and Technology (CONACYT)‑National Institute of Genomic Medicine (INMEGEN), Mexico City 14610, Mexico
Published online on: October 23, 2020 https://doi.org/10.3892/etm.2020.9384
Article Number: 254
Copyright: © Parra‑Torres et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Bone formation is a dynamic process directed by osteoblast activity. The transition from the proliferation to differentiation stage during osteoblast maturation involves the downregulation of the Wnt/β‑catenin signaling pathway, and extracellular antagonists are important for the regulation of Wnt signaling. However, the expression levels of Wnt antagonists in these stages of human osteoblast maturation have not been fully elucidated. Therefore, the aim of the present study was to investigate the expression levels of extracellular Wnt antagonists during proliferation and differentiation in osteoblast‑like cell lines. The results demonstrated an overlap between the differential expression of secreted Frizzled‑related protein (SFPR)2, SFRP3, SFRP4 and Dickkopf (DKK) 2 genes during the differentiation stage in the MG‑63 and Saos‑2 cells. Furthermore, high expression levels of DKK3 in MG‑63 cells, Wnt inhibitory factor 1 (WIF1) in Saos‑2 cells and DKK4 in hFOB 1.19 cells during the same stage (differentiation), were observed. The upregulated expression levels of Wnt antagonists were also correlated with the high expression of anxin 2 during the differentiation stage. These findings suggested that Wnt‑related antagonists could modulate the Wnt/β‑catenin signaling pathway. By contrast, DKK1 was the only gene that was found to be upregulated during the proliferation stage in hFOB 1.19 and Saos‑2 cells. To the best of our knowledge, the present study provides, for the first time, the expression profile of Wnt antagonists during the proliferation stage and the initial phases of differentiation in osteoblast‑like cell lines. The current results offer a basis to investigate potential targets for bone‑related Wnt‑signaling modulation in bone metabolism research.

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