Open Access

Astragalus polysaccharide enhances the immune function of RAW264.7 macrophages via the NF‑κB p65/MAPK signaling pathway

  • Authors:
    • Shibin Feng
    • Hongyan Ding
    • Leihong Liu
    • Chenglu Peng
    • Yingying Huang
    • Fuchao Zhong
    • Wei Li
    • Tingting Meng
    • Jinchun Li
    • Xichun Wang
    • Yu Li
    • Jinjie Wu
  • View Affiliations

  • Published online on: November 5, 2020     https://doi.org/10.3892/etm.2020.9452
  • Article Number: 20
  • Copyright: © Feng et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The aim of the present study was to investigate the immunoregulatory effects of Astragalus polysaccharide (APS) on RAW264.7 cells. The production of cytokines by RAW264.7 cells was analyzed using ELISA, while cell viability and optimal concentration of APS were assessed using the Cell Counting Kit‑8 assay. In addition, the mRNA levels of IL‑6, inducible nitric oxide synthase (iNOS) and TNF‑α were determined by reverse transcription‑quantitative PCR analysis. The levels of co‑stimulatory molecules and cell cycle distribution were assessed by flow cytometry. Electrophoretic mobility shift assay was used to determine the effects of APS on p65 expression. Compared with controls, APS enhanced the production of NO, the gene expression of TNF‑α, IL‑6 and iNOS and the protein levels of phosphorylated p65, p38, Jun N‑terminal kinase and extracellular signal regulated kinase in RAW264.7 cells, whereas these effects of APS were alleviated by pyrrolidine dithiocarbamate. The results of the present study indicated that the immunoregulatory effects of APS are mediated, at least in part, via the activation of the NF‑κB p65/MAPK signaling pathway.
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January-2021
Volume 21 Issue 1

Print ISSN: 1792-0981
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Spandidos Publications style
Feng S, Ding H, Liu L, Peng C, Huang Y, Zhong F, Li W, Meng T, Li J, Wang X, Wang X, et al: <em>Astragalus</em> polysaccharide enhances the immune function of RAW264.7 macrophages via the NF‑&kappa;B p65/MAPK signaling pathway. Exp Ther Med 21: 20, 2021
APA
Feng, S., Ding, H., Liu, L., Peng, C., Huang, Y., Zhong, F. ... Wu, J. (2021). <em>Astragalus</em> polysaccharide enhances the immune function of RAW264.7 macrophages via the NF‑&kappa;B p65/MAPK signaling pathway. Experimental and Therapeutic Medicine, 21, 20. https://doi.org/10.3892/etm.2020.9452
MLA
Feng, S., Ding, H., Liu, L., Peng, C., Huang, Y., Zhong, F., Li, W., Meng, T., Li, J., Wang, X., Li, Y., Wu, J."<em>Astragalus</em> polysaccharide enhances the immune function of RAW264.7 macrophages via the NF‑&kappa;B p65/MAPK signaling pathway". Experimental and Therapeutic Medicine 21.1 (2021): 20.
Chicago
Feng, S., Ding, H., Liu, L., Peng, C., Huang, Y., Zhong, F., Li, W., Meng, T., Li, J., Wang, X., Li, Y., Wu, J."<em>Astragalus</em> polysaccharide enhances the immune function of RAW264.7 macrophages via the NF‑&kappa;B p65/MAPK signaling pathway". Experimental and Therapeutic Medicine 21, no. 1 (2021): 20. https://doi.org/10.3892/etm.2020.9452