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miR‑155‑5p regulates macrophage M1 polarization and apoptosis in the synovial fluid of patients with knee osteoarthritis

  • Authors:
    • Gui-Shi Li
    • Lei Cui
    • Guang-Da Wang
  • View Affiliations / Copyright

    Affiliations: Department of Joint Orthopaedics, Yantai Yuhuangding Hospital, Yantai, Shandong 264000, P.R. China, Department of Opthalmology Surgery, Yantai Yuhuangding Hospital, Yantai, Shandong 264000, P.R. China
    Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 68
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    Published online on: November 23, 2020
       https://doi.org/10.3892/etm.2020.9500
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Abstract

Osteoarthritis (OA) is one of the most prevalent degenerative joint diseases that affects millions of individuals worldwide. During OA, proinflammatory factors (including IL‑1, IL‑6, IL‑17 and TNF‑α) are released from chondrocytes and proliferating synoviocytes potentiate the proinflammatory microenvironment of the synovial fluid (SF). The altered SF microenvironment affects the infiltration, polarization and apoptosis of macrophages, though the underlying mechanisms are not completely understood. In the present study, the hypothesis that the knee synovial fluid of patients with knee osteoarthritis (KOA SF) promotes the polarization of peripheral blood mononuclear cell (PBMC)‑derived M1 macrophages and inhibits PBMC‑derived macrophage apoptosis was investigated. KOA SF increased PBMC‑derived macrophage M1 polarization via the microRNA (miR)‑155‑5p/suppressor of cytokine signaling 1 signaling pathway. Caspase‑3 (CASP3) was identified as a novel target of miR‑155‑5p, where KOA SF inhibited macrophage apoptosis via the miR‑155‑5p/CASP3 signaling pathway. The results suggested that the proinflammatory environment of KOA SF promoted macrophage M1 polarization and reduced macrophage apoptosis via miR‑155‑5p. The results provided a potential explanation for the increased number of M1 macrophages observed in KOA SF during OA. In addition, the present study suggested that miR‑155‑5p may serve as a potential therapeutic target for KOA.
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Copy and paste a formatted citation
Spandidos Publications style
Li G, Cui L and Wang G: miR‑155‑5p regulates macrophage M1 polarization and apoptosis in the synovial fluid of patients with knee osteoarthritis. Exp Ther Med 21: 68, 2021.
APA
Li, G., Cui, L., & Wang, G. (2021). miR‑155‑5p regulates macrophage M1 polarization and apoptosis in the synovial fluid of patients with knee osteoarthritis. Experimental and Therapeutic Medicine, 21, 68. https://doi.org/10.3892/etm.2020.9500
MLA
Li, G., Cui, L., Wang, G."miR‑155‑5p regulates macrophage M1 polarization and apoptosis in the synovial fluid of patients with knee osteoarthritis". Experimental and Therapeutic Medicine 21.1 (2021): 68.
Chicago
Li, G., Cui, L., Wang, G."miR‑155‑5p regulates macrophage M1 polarization and apoptosis in the synovial fluid of patients with knee osteoarthritis". Experimental and Therapeutic Medicine 21, no. 1 (2021): 68. https://doi.org/10.3892/etm.2020.9500
Copy and paste a formatted citation
x
Spandidos Publications style
Li G, Cui L and Wang G: miR‑155‑5p regulates macrophage M1 polarization and apoptosis in the synovial fluid of patients with knee osteoarthritis. Exp Ther Med 21: 68, 2021.
APA
Li, G., Cui, L., & Wang, G. (2021). miR‑155‑5p regulates macrophage M1 polarization and apoptosis in the synovial fluid of patients with knee osteoarthritis. Experimental and Therapeutic Medicine, 21, 68. https://doi.org/10.3892/etm.2020.9500
MLA
Li, G., Cui, L., Wang, G."miR‑155‑5p regulates macrophage M1 polarization and apoptosis in the synovial fluid of patients with knee osteoarthritis". Experimental and Therapeutic Medicine 21.1 (2021): 68.
Chicago
Li, G., Cui, L., Wang, G."miR‑155‑5p regulates macrophage M1 polarization and apoptosis in the synovial fluid of patients with knee osteoarthritis". Experimental and Therapeutic Medicine 21, no. 1 (2021): 68. https://doi.org/10.3892/etm.2020.9500
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