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IFN‑γ and IL‑18 in conditioned media of parasite‑infected host and IL‑21‑silenced colorectal cancer cells

  • Authors:
    • Peng Ge
    • Ching Yi Ong
    • Abdalla Eshtiyag Abdalkareem
    • Boon Yin Khoo
    • Bo Yuan
  • View Affiliations / Copyright

    Affiliations: Department of General Surgery, Xi'an Central Hospital, Xi'an, Shaanxi 710003, P.R. China, Institute for Research in Molecular Medicine (INFORMM), Universiti Sains Malaysia, Penang 11800, Malaysia
    Copyright: © Ge et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 103
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    Published online on: November 27, 2020
       https://doi.org/10.3892/etm.2020.9535
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Abstract

The presence of certain soluble factors may provide a possible selective advantage for a parasite to gradually modify cell proliferation in neighbouring cells, which may result in chronic diseases. These soluble factors present in the conditioned medium also allow the parasite to invade rapidly into more host cells. The present study aimed to determine the levels of a group of type 1 T helper (Th1) cytokines in the conditioned media of host cells infected with parasites and in IL‑21‑silenced colorectal cancer cells. The conditioned media of human foreskin fibroblasts (HFFs) parasitized with the RH and ME49 strains of Toxoplasma gondii for 10 days were prepared, and subsequently the levels of the Th1 cytokines in the conditioned media were determined by ELISA. HFFs were incubated with the growth media containing selected soluble factors, and cell proliferation markers were subsequently analysed by reverse transcription‑quantitative PCR. The mRNA expression level of cell proliferation markers was also examined in IL‑21‑silenced HCT116 cells, where the levels of soluble factors in the conditioned media were also determined as aforementioned. The results of the present study demonstrated that HFFs parasitized with ME49 released elevated levels of IFN‑γ and lower levels of IL‑18 into the conditioned medium compared with the controls. These phenomena were not observed in the conditioned medium of HFFs parasitized with RH. Similar levels of these soluble factors were also detected in the conditioned medium of IL‑21‑silenced HCT116 cells. The results of the present study also revealed that Ki67 and proliferating cell nuclear antigen mRNA expression was altered in host cells incubated with various levels of IFN‑γ and IL‑18, as well as in IL‑21‑silenced HCT116 cells compared with the respective controls. In conclusion, the current study provided preliminary evidence on the fundamental molecular mechanisms of host‑parasite interactions that result in chronic diseases, which may aid in the treatment of these diseases in the relevant endemic regions.
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Copy and paste a formatted citation
Spandidos Publications style
Ge P, Ong C, Abdalkareem A, Khoo B and Yuan B: IFN‑γ and IL‑18 in conditioned media of parasite‑infected host and IL‑21‑silenced colorectal cancer cells. Exp Ther Med 21: 103, 2021.
APA
Ge, P., Ong, C., Abdalkareem, A., Khoo, B., & Yuan, B. (2021). IFN‑γ and IL‑18 in conditioned media of parasite‑infected host and IL‑21‑silenced colorectal cancer cells. Experimental and Therapeutic Medicine, 21, 103. https://doi.org/10.3892/etm.2020.9535
MLA
Ge, P., Ong, C., Abdalkareem, A., Khoo, B., Yuan, B."IFN‑γ and IL‑18 in conditioned media of parasite‑infected host and IL‑21‑silenced colorectal cancer cells". Experimental and Therapeutic Medicine 21.2 (2021): 103.
Chicago
Ge, P., Ong, C., Abdalkareem, A., Khoo, B., Yuan, B."IFN‑γ and IL‑18 in conditioned media of parasite‑infected host and IL‑21‑silenced colorectal cancer cells". Experimental and Therapeutic Medicine 21, no. 2 (2021): 103. https://doi.org/10.3892/etm.2020.9535
Copy and paste a formatted citation
x
Spandidos Publications style
Ge P, Ong C, Abdalkareem A, Khoo B and Yuan B: IFN‑γ and IL‑18 in conditioned media of parasite‑infected host and IL‑21‑silenced colorectal cancer cells. Exp Ther Med 21: 103, 2021.
APA
Ge, P., Ong, C., Abdalkareem, A., Khoo, B., & Yuan, B. (2021). IFN‑γ and IL‑18 in conditioned media of parasite‑infected host and IL‑21‑silenced colorectal cancer cells. Experimental and Therapeutic Medicine, 21, 103. https://doi.org/10.3892/etm.2020.9535
MLA
Ge, P., Ong, C., Abdalkareem, A., Khoo, B., Yuan, B."IFN‑γ and IL‑18 in conditioned media of parasite‑infected host and IL‑21‑silenced colorectal cancer cells". Experimental and Therapeutic Medicine 21.2 (2021): 103.
Chicago
Ge, P., Ong, C., Abdalkareem, A., Khoo, B., Yuan, B."IFN‑γ and IL‑18 in conditioned media of parasite‑infected host and IL‑21‑silenced colorectal cancer cells". Experimental and Therapeutic Medicine 21, no. 2 (2021): 103. https://doi.org/10.3892/etm.2020.9535
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