Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Experimental and Therapeutic Medicine
Join Editorial Board Propose a Special Issue
Print ISSN: 1792-0981 Online ISSN: 1792-1015
Journal Cover
February-2021 Volume 21 Issue 2

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
February-2021 Volume 21 Issue 2

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article Open Access

Regulation of dimethylarginine dimethylaminohydrolase 2 expression by NF‑κB acetylation

  • Authors:
    • Jiaqi Li
    • Lu Sun
    • Yinghui Li
  • View Affiliations / Copyright

    Affiliations: Department of Medical Genetics, School of Life Science, China Medical University, Shenyang, Liaoning 110122, P.R. China
    Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 114
    |
    Published online on: December 3, 2020
       https://doi.org/10.3892/etm.2020.9546
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Nitric oxide (NO) serves a crucial role in the kidney and is synthesized by NO synthase (NOS). Asymmetrical dimethylarginine is an endogenous inhibitor of NOS that is metabolized by dimethylarginine dimethylaminohydrolase (DDAH). To investigate the role of acetylation in DDAH2 expression, 293 cells were treated with trichostatin A (TSA), a deacetylase inhibitor and the mRNA and protein levels were assessed using quantitative PCR and western blotting respectively. Its promoter activity was detected using a luciferase assay. The effect of TSA on NF‑κB acetylation was tested after immunoprecipitation. The binding of NF‑κB to the DDAH2 promoter was analyzed using an electrophoretic mobility shift assay and chromatin immunoprecipitation. TSA upregulated DDAH2 expression and transcriptional activity of the DDAH2 promoter through a NF‑κB responsive element, which is located at the ‑1582 to ‑1573 position of the DDAH2 promoter. Furthermore, TSA treatment promoted NF‑κB acetylation, resulting in enhanced NF‑κB binding affinity to its binding site both in vitro and in vivo. Taken together, the present study demonstrated that NF‑κB acetylation upregulated DDAH2 expression by enhancing the binding ability of NF‑κB to the DDAH2 promoter, resulting in increased promoter activity. The results provided a possible mechanism underlying the regulation of NO production in renal cells and a potential target for treating certain NO‑associated renal disorders.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

View References

1 

Zou AP and Cowley AW Jr: Role of nitric oxide in the control of renal function and salt sensitivity. Curr Hypertens Rep. 1:178–186. 1999.PubMed/NCBI View Article : Google Scholar

2 

Monzon CM and Garvin JL: Nitric oxide decreases the permselectivity of the paracellular pathway in thick ascending limbs. Hypertension. 65:1245–1250. 2015.PubMed/NCBI View Article : Google Scholar

3 

Garcia NH, Stoos BA, Carretero OA and Garvin JL: Mechanism of the nitric oxide-induced blockade of collecting duct water permeability. Hypertension. 27:679–683. 1996.PubMed/NCBI View Article : Google Scholar

4 

Tain YL and Hsu CN: Toxic dimethylarginines: Asymmetric dimethylarginine (ADMA) and symmetric dimethylarginine (SDMA). Toxins (Basel). 9(92)2017.PubMed/NCBI View Article : Google Scholar

5 

Jayachandran I, Sundararajan S, Paramasivam P, Venkatesan B, Subramanian SC, Balasubramanyam M, Mohan V and Manickam N: Association of circulatory asymmetric dimethylarginine (ADMA) with diabetic nephropathy in Asian Indians and its causative role in renal cell injury. Clinical Biochem. 50:835–842. 2017.PubMed/NCBI View Article : Google Scholar

6 

Palm F, Onozato ML, Luo Z and Wilcox CS: Dimethylarginine dimethylaminohydrolase (DDAH): Expression, regulation, and function in the cardiovascular and renal systems. Am J Physiol Heart Circ Physiol. 293:H3227–H3245. 2007.PubMed/NCBI View Article : Google Scholar

7 

Tojo A, Welch WJ, Bremer V, Kimoto M, Kimura K, Omata M, Ogawa T, Vallance P and Wilcox CS: Colocalization of demethylating enzymes and NOS and functional effects of methylarginines in rat kidney. Kidney Int. 52:1593–1601. 1997.PubMed/NCBI View Article : Google Scholar

8 

Nijveldt RJ, Teerlink T, Siroen MP, van Lambalgen AA, Rauwerda JA and van Leeuwen PA: The liver is an important organ in the metabolism of asymmetrical dimethylarginine (ADMA). Clin Nutr. 22:17–22. 2003.PubMed/NCBI View Article : Google Scholar

9 

Förstermann U and Sessa WC: Nitric oxide synthases: Regulation and function. Eur Heart J. 33:829–37. 2012.PubMed/NCBI View Article : Google Scholar

10 

Zhang Z, Zhu LL, Jiang HS, Chen H, Chen Y and Dai YT: Demethylation treatment restores erectile function in a rat model of hyperhomocysteinemia. Asian J Androl. 18:763–768. 2016.PubMed/NCBI View Article : Google Scholar

11 

Zhang JG, Liu JX, Li ZH, Wang LZ, Jiang YD and Wang SR: Dysfunction of endothelial NO system originated from homocysteine-induced aberrant methylation pattern in promoter region of DDAH2 gene. Chin Med J. 120:2132–7. 2007.PubMed/NCBI

12 

Tomikawa J, Fukatsu K, Tanaka S and Shiota K: DNA methylation-dependent epigenetic regulation of dimethylarginine dimethylaminohydrolase 2 gene in trophoblast cell lineage. J Biol Chem. 281:12163–12169. 2006.PubMed/NCBI View Article : Google Scholar

13 

Li Y, Li C, Sun L, Chu G, Li J, Chen F, Li G and Zhao Y: Role of p300 in regulating neuronal nitric oxide synthase gene expression through nuclear factor-κB-mediated way in neuronal cells. Neuroscience. 248:681–689. 2013.PubMed/NCBI View Article : Google Scholar

14 

Chen J, Zhang J, Shaik NF, Yi B, Wei X, Yang XF, Naik UP, Summer R, Yan G, Xu X and Sun J: The histone deacetylase inhibitor tubacin mitigates endothelial dysfunction by up-regulating the expression of endothelial nitric oxide synthase. J Biol Chem. 294:19565–19576. 2019.PubMed/NCBI View Article : Google Scholar

15 

Chen L, Fischle W, Verdin E and Greene WC: Duration of nuclear NF-kappaB action regulated by reversible acetylation. Science. 293:1653–1657. 2001.PubMed/NCBI View Article : Google Scholar

16 

Huang W, Zhao S, Ammanamanchi S, Brattain M, Venkatasubbarao K and Freeman JW: Trichostatin A induces transforming growth factor beta type II receptor promoter activity and acetylation of Sp1 by recruitment of PCAF/p300 to a Sp1.NF-Y complex. J Biol Chem. 280:10047–10054. 2005.PubMed/NCBI View Article : Google Scholar

17 

Izumi H, Ohta R, Nagatani G, Ise T, Nakayama Y, Nomoto M and Kohno K: p300/CBP-associated factor (P/CAF) interacts with nuclear respiratory factor-1 to regulate the UDP-N-acetyl-alpha-d-galactosamine: Polypeptide N-acetylgalactosaminyltransferase-3 gene. Biochem J. 373:713–722. 2003.PubMed/NCBI View Article : Google Scholar

18 

Lee JS, Galvin KM, See RH, Eckner R, Livingston D, Moran E and Shi Y: Relief of YY1 transcriptional repression by adenovirus E1A is mediated by E1A-associated protein p300. Genes Dev. 9:1188–1198. 1995.PubMed/NCBI View Article : Google Scholar

19 

Li Y, Zhao Y, Li G, Wang J, Li T, Li W and Lu J: Regulation of neuronal nitric oxide synthase exon 1f gene expression by nuclear factor-kappaB acetylation in human neuroblastoma cells. J Neurochem. 101:1194–1204. 2007.PubMed/NCBI View Article : Google Scholar

20 

Deng WG and Wu KK: Regulation of inducible nitric oxide synthase expression by p300 and p50 acetylation. J Immunol. 171:6581–6588. 2003.PubMed/NCBI View Article : Google Scholar

21 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001.PubMed/NCBI View Article : Google Scholar

22 

Korneluk RG, Quan F and Gravel RA: Rapid and reliable dideoxy sequencing of double-stranded DNA. Gene. 40:317–323. 1985.PubMed/NCBI View Article : Google Scholar

23 

Sengüven B, Baris E, Oygur T and Berktas M: Comparison of methods for the extraction of DNA from formalin-fixed, paraffin-embedded archival tissues. Int J Med Sci. 11:494–499. 2014.PubMed/NCBI View Article : Google Scholar

24 

Haring M, Offermann S, Danker T, Horst I, Peterhansel C and Stam M: Chromatin immunoprecipitation: Optimization, quantitative analysis and data normalization. Plant Methods. 3(11)2007.PubMed/NCBI View Article : Google Scholar

25 

Yoshida M, Kijima M, Akita M and Beppu T: Potent and specific inhibition of mammalian histone deacetylase both in vivo and in vitro by trichostatin A. J Biol Chem. 265:17174–17179. 1990.PubMed/NCBI

26 

Guijarro C and Egido J: Transcription factor-kappa B (NF-kappa B) and renal disease. Kidney Int. 59:415–424. 2001.PubMed/NCBI View Article : Google Scholar

27 

Queisser N and Schupp N: Aldosterone, oxidative stress, and NF-κB activation in hypertension-related cardiovascular and renal diseases. Free Radic Biol Med. 53:314–327. 2012.PubMed/NCBI View Article : Google Scholar

28 

Shang G, Gao P, Zhao Z, Chen Q, Jiang T, Zhang N and Li H: 3,5-Diiodo-l-thyronine ameliorates diabetic nephropathy in streptozotocin-induced diabetic rats. Biochim Biophys Acta. 1832:674–684. 2013.PubMed/NCBI View Article : Google Scholar

29 

Liu R, Zhong Y, Li X, Chen H, Jim B, Zhou MM, Chuang PY and He JC: Role of transcription factor acetylation in diabetic kidney disease. Diabetes. 63:2440–2453. 2014.PubMed/NCBI View Article : Google Scholar

30 

Jung YJ, Lee JE, Lee AS, Kang KP, Lee S, Park SK, Lee SY, Han MK, Kim DH and Kim W: SIRT1 overexpression decreases cisplatin-induced acetylation of NF-κB p65 subunit and cytotoxicity in renal proximal tubule cells. Biochem Biophys Res Commun. 419:206–210. 2012.PubMed/NCBI View Article : Google Scholar

31 

Turner BM, Birley AJ and Lavender J: Histone H4 isoforms acetylated at specific lysine residues define individual chromosomes and chromatin domains in Drosophila polytene nuclei. Cell. 69:375–384. 1992.PubMed/NCBI View Article : Google Scholar

32 

Turner BM and Fellows G: Specific antibodies reveal ordered and cell-cycle-related use of histone-H4 acetylation sites in mammalian cells. Eur J Biochem. 179:131–139. 1989.PubMed/NCBI View Article : Google Scholar

33 

Verdin E and Ott M: 50 years of protein acetylation: From gene regulation to epigenetics, metabolism and beyond. Nat Rev Mol Cell Biol. 16:258–264. 2015.PubMed/NCBI View Article : Google Scholar

34 

Gu W and Roeder RG: Activation of p53 sequence-specific DNA binding by acetylation of the p53 C-terminal domain. Cell. 90:595–606. 1997.PubMed/NCBI View Article : Google Scholar

35 

Kiernan RE, Vanhulle C, Schiltz L, Adam E, Xiao H, Maudoux F, Calomme C, Burny A, Nakatani Y, Jeang KT, et al: HIV-1 tat transcriptional activity is regulated by acetylation. EMBO J. 18:6106–6118. 1999.PubMed/NCBI View Article : Google Scholar

36 

Yang XJ and Seto E: HATs and HDACs: From structure, function and regulation to novel strategies for therapy and prevention. Oncogene. 26:5310–5318. 2007.PubMed/NCBI View Article : Google Scholar

37 

Huynh NC, Everts V and Ampornaramveth RS: Histone deacetylases and their roles in mineralized tissue regeneration. Bone Rep. 7:33–40. 2017.PubMed/NCBI View Article : Google Scholar

38 

Zhang H and Sun SC: NF-κB in inflammation and renal diseases. Cell Biosci. 5(63)2015.PubMed/NCBI View Article : Google Scholar

39 

Henke N, Schmidt-Ullrich R, Dechend R, Park JK, Qadri F, Wellner M, Obst M, Gross V, Dietz R, Luft FC, et al: Vascular endothelial cell-specific NF-kappaB suppression attenuates hypertension-induced renal damage. Circ Res. 101:268–276. 2007.PubMed/NCBI View Article : Google Scholar

40 

Jones LC, Tran CT, Leiper JM, Hingorani AD and Vallance P: Common genetic variation in a basal promoter element alters DDAH2 expression in endothelial cells. Biochem Biophys Res Commun. 310:836–843. 2003.PubMed/NCBI View Article : Google Scholar

41 

Andreozzi F, Presta I, Mannino GC, Scarpelli D, Di Silvestre S, Di Pietro N, Succurro E, Sciacqua A, Pandolfi A, Consoli A, et al: A functional variant of the dimethylarginine dimethylaminohydrolase-2 gene is associated with insulin sensitivity. PLoS One. 7(e36224)2012.PubMed/NCBI View Article : Google Scholar

42 

Park JM, Jo SH, Kim MY, Kim TH and Ahn YH: Role of transcription factor acetylation in the regulation of metabolic homeostasis. Protein Cell. 6:804–813. 2015.PubMed/NCBI View Article : Google Scholar

43 

Ma Z, Chalkley RJ and Vosseller K: Hyper-O-GlcNAcylation activates nuclear factor κ-light-chain-enhancer of activated B cells (NF-κB) signaling through interplay with phosphorylation and acetylation. J Biol Chem. 292:9150–9163. 2017.PubMed/NCBI View Article : Google Scholar

44 

Balasubramanian V, Mehta G, Jones H, Sharma V, Davies NA, Jalan R and Mookerjee RP: Post-transcriptional regulation of hepatic DDAH1 with TNF blockade leads to improved eNOS function and reduced portal pressure in cirrhotic rats. Sci Rep. 7(17900)2017.PubMed/NCBI View Article : Google Scholar

45 

Hsu CN and Tain YL: Regulation of nitric oxide production in the developmental programming of hypertension and kidney disease. Int J Mol Sci. 20(681)2019.PubMed/NCBI View Article : Google Scholar

46 

Ahmad A, Dempsey SK, Daneva Z, Azam M, Li N, Li PL and Ritter JK: Role of Nitric Oxide in the Cardiovascular and Renal Systems. Int J Mo Sci. 19(2605)2018.PubMed/NCBI View Article : Google Scholar

47 

Lin HH, Lee TS, Lin SJ, Yeh YC, Lu TM and Hsu CP: DDAH-2 alleviates contrast medium iopromide-induced acute kidney injury through nitric oxide synthase. Clin Sci (Lond). 133:2361–2378. 2019.PubMed/NCBI View Article : Google Scholar

48 

Wagner L, Riggleman A, Erdely A, Couser W and Baylis C: Reduced nitric oxide synthase activity in rats with chronic renal disease due to glomerulonephritis. Kidney Int. 62:532–536. 2002.PubMed/NCBI View Article : Google Scholar

49 

Staab EB, Weigel J, Xiao F, Madayiputhiya N, Wyatt TA and Wells SM: Asymmetric dimethyl-arginine metabolism in a murine model of cigarette smoke-mediated lung inflammation. J Immunotoxicol. 12:273–282. 2015.PubMed/NCBI View Article : Google Scholar

50 

Lin YC, Boone M, Meuris L, Lemmens I, Van Roy N, Soete A, Reumers J, Moisse M, Plaisance S, Drmanac R, et al: Genome dynamics of the human embryonic kidney 293 lineage in response to cell biology manipulations. Nat Commun. 5(4767)2014.PubMed/NCBI View Article : Google Scholar

51 

Stepanenko AA and Dmitrenko VV: HEK293 in cell biology and cancer research: Phenotype, karyotype, tumorigenicity, and stress-induced genome-phenotype evolution. Gene. 569:182–190. 2015.PubMed/NCBI View Article : Google Scholar

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Li J, Sun L and Li Y: Regulation of dimethylarginine dimethylaminohydrolase 2 expression by NF‑κB acetylation. Exp Ther Med 21: 114, 2021.
APA
Li, J., Sun, L., & Li, Y. (2021). Regulation of dimethylarginine dimethylaminohydrolase 2 expression by NF‑κB acetylation. Experimental and Therapeutic Medicine, 21, 114. https://doi.org/10.3892/etm.2020.9546
MLA
Li, J., Sun, L., Li, Y."Regulation of dimethylarginine dimethylaminohydrolase 2 expression by NF‑κB acetylation". Experimental and Therapeutic Medicine 21.2 (2021): 114.
Chicago
Li, J., Sun, L., Li, Y."Regulation of dimethylarginine dimethylaminohydrolase 2 expression by NF‑κB acetylation". Experimental and Therapeutic Medicine 21, no. 2 (2021): 114. https://doi.org/10.3892/etm.2020.9546
Copy and paste a formatted citation
x
Spandidos Publications style
Li J, Sun L and Li Y: Regulation of dimethylarginine dimethylaminohydrolase 2 expression by NF‑κB acetylation. Exp Ther Med 21: 114, 2021.
APA
Li, J., Sun, L., & Li, Y. (2021). Regulation of dimethylarginine dimethylaminohydrolase 2 expression by NF‑κB acetylation. Experimental and Therapeutic Medicine, 21, 114. https://doi.org/10.3892/etm.2020.9546
MLA
Li, J., Sun, L., Li, Y."Regulation of dimethylarginine dimethylaminohydrolase 2 expression by NF‑κB acetylation". Experimental and Therapeutic Medicine 21.2 (2021): 114.
Chicago
Li, J., Sun, L., Li, Y."Regulation of dimethylarginine dimethylaminohydrolase 2 expression by NF‑κB acetylation". Experimental and Therapeutic Medicine 21, no. 2 (2021): 114. https://doi.org/10.3892/etm.2020.9546
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team