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Article Open Access

MicroRNA‑451a prevents cutaneous squamous cell carcinoma progression via the 3‑phosphoinositide‑dependent protein kinase‑1‑mediated PI3K/AKT signaling pathway

Retraction in: /10.3892/etm.2024.12568
  • Authors:
    • Jixing Fu
    • Jianhua Zhao
    • Huamin Zhang
    • Xiaoli Fan
    • Wenjun Geng
    • Shaohua Qiao
  • View Affiliations / Copyright

    Affiliations: Department of Dermatology, Liaocheng Second People's Hospital, Shandong First Medical University Affiliated Liaocheng Second Hospital, Linqing, Shandong 252601, P.R. China
    Copyright: © Fu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 116
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    Published online on: December 3, 2020
       https://doi.org/10.3892/etm.2020.9548
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Abstract

The role of microRNAs (miRNAs/miRs) in governing the progression of cutaneous squamous cell carcinoma (cSCC) has been the focus of recent studies. However, the functional role of miR‑451a in cSCC growth remains poorly understood. Therefore, the present study aimed to determine the expression levels of miR‑451a in cSCC cell lines and the involvement of miR‑451a in cSCC progression. The results revealed that the expression levels of miR‑451a were downregulated in cSCC tissues and cell lines, and that this subsequently upregulated 3‑phosphoinositide‑dependent protein kinase‑1 (PDPK1) expression levels. PDPK1 was validated as a direct target of miR‑451a in cSCC using bioinformatics software Starbase, dual‑luciferase reporter gene assays and western blotting. Additionally, CCK‑8, EdU and Transwell assays, as well as flow cytometry and Hoechst 3325 staining, were performed to assess the malignant aggressiveness of cSCC cells. Overexpression of miR‑451a was demonstrated to impair the proliferation, migration, invasion and epithelial‑mesenchymal transition (EMT), and promoted apoptosis in cSCC cells by interacting with PDPK1, possibly by direct targeting. Furthermore, the western blotting results indicated that miR‑451a overexpression may block the PI3K/AKT signaling pathway by interacting with PDPK1. In conclusion, the findings of the present study suggested that miR‑451a may prevent the proliferation, migration, invasion and EMT of cSCC cells through the PDPK1‑mediated PI3K/AKT signaling pathway, which may offer potential therapeutic targets for the treatment of cSCC.
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Copy and paste a formatted citation
Spandidos Publications style
Fu J, Zhao J, Zhang H, Fan X, Geng W and Qiao S: MicroRNA‑451a prevents cutaneous squamous cell carcinoma progression via the 3‑phosphoinositide‑dependent protein kinase‑1‑mediated PI3K/AKT signaling pathway Retraction in /10.3892/etm.2024.12568. Exp Ther Med 21: 116, 2021.
APA
Fu, J., Zhao, J., Zhang, H., Fan, X., Geng, W., & Qiao, S. (2021). MicroRNA‑451a prevents cutaneous squamous cell carcinoma progression via the 3‑phosphoinositide‑dependent protein kinase‑1‑mediated PI3K/AKT signaling pathway Retraction in /10.3892/etm.2024.12568. Experimental and Therapeutic Medicine, 21, 116. https://doi.org/10.3892/etm.2020.9548
MLA
Fu, J., Zhao, J., Zhang, H., Fan, X., Geng, W., Qiao, S."MicroRNA‑451a prevents cutaneous squamous cell carcinoma progression via the 3‑phosphoinositide‑dependent protein kinase‑1‑mediated PI3K/AKT signaling pathway Retraction in /10.3892/etm.2024.12568". Experimental and Therapeutic Medicine 21.2 (2021): 116.
Chicago
Fu, J., Zhao, J., Zhang, H., Fan, X., Geng, W., Qiao, S."MicroRNA‑451a prevents cutaneous squamous cell carcinoma progression via the 3‑phosphoinositide‑dependent protein kinase‑1‑mediated PI3K/AKT signaling pathway Retraction in /10.3892/etm.2024.12568". Experimental and Therapeutic Medicine 21, no. 2 (2021): 116. https://doi.org/10.3892/etm.2020.9548
Copy and paste a formatted citation
x
Spandidos Publications style
Fu J, Zhao J, Zhang H, Fan X, Geng W and Qiao S: MicroRNA‑451a prevents cutaneous squamous cell carcinoma progression via the 3‑phosphoinositide‑dependent protein kinase‑1‑mediated PI3K/AKT signaling pathway Retraction in /10.3892/etm.2024.12568. Exp Ther Med 21: 116, 2021.
APA
Fu, J., Zhao, J., Zhang, H., Fan, X., Geng, W., & Qiao, S. (2021). MicroRNA‑451a prevents cutaneous squamous cell carcinoma progression via the 3‑phosphoinositide‑dependent protein kinase‑1‑mediated PI3K/AKT signaling pathway Retraction in /10.3892/etm.2024.12568. Experimental and Therapeutic Medicine, 21, 116. https://doi.org/10.3892/etm.2020.9548
MLA
Fu, J., Zhao, J., Zhang, H., Fan, X., Geng, W., Qiao, S."MicroRNA‑451a prevents cutaneous squamous cell carcinoma progression via the 3‑phosphoinositide‑dependent protein kinase‑1‑mediated PI3K/AKT signaling pathway Retraction in /10.3892/etm.2024.12568". Experimental and Therapeutic Medicine 21.2 (2021): 116.
Chicago
Fu, J., Zhao, J., Zhang, H., Fan, X., Geng, W., Qiao, S."MicroRNA‑451a prevents cutaneous squamous cell carcinoma progression via the 3‑phosphoinositide‑dependent protein kinase‑1‑mediated PI3K/AKT signaling pathway Retraction in /10.3892/etm.2024.12568". Experimental and Therapeutic Medicine 21, no. 2 (2021): 116. https://doi.org/10.3892/etm.2020.9548
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