Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Experimental and Therapeutic Medicine
Join Editorial Board Propose a Special Issue
Print ISSN: 1792-0981 Online ISSN: 1792-1015
Journal Cover
February-2021 Volume 21 Issue 2

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
February-2021 Volume 21 Issue 2

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article Open Access

Diagnostic value of candidate noncoding RNAs in leukocytes of patients with gestational diabetes mellitus

  • Authors:
    • Jialei Hu
    • Hanyou Mu
    • Linshan Gao
    • Ying Pan
    • Chuanfei Wu
    • Dong Zhang
    • Qiaojun Chen
    • Honghui Ding
  • View Affiliations / Copyright

    Affiliations: Clinical Laboratory, Yiwu Maternal and Child Health Hospital, Jinhua, Zhejiang 321000, P.R. China, Department of Pediatrics, Yiwu Maternal and Child Health Hospital, Jinhua, Zhejiang 321000, P.R. China
    Copyright: © Hu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 145
    |
    Published online on: December 16, 2020
       https://doi.org/10.3892/etm.2020.9576
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Noncoding RNAs (ncRNAs) are involved in the pathological processes of various diseases. The aim of the present study was to verify the expression levels and the diagnostic value of two candidate ncRNAs in the blood leukocytes of patients with gestational diabetes mellitus (GDM) compared to healthy controls. The long ncRNA paired box 8 antisense 1 (Pax8‑AS1) and the microRNA miR‑4646 were selected, which were identified to be associated with GDM by bioinformatics analysis of a dataset from the Gene Expression Omnibus GEO database. By using reverse transcription‑quantitative PCR, the expression levels of Pax8‑AS1 and miR‑4646 were analysed in leukocytes of patients with GDM (n=35) and normal pregnant females (n=35). The results indicated a significant decrease in the expression levels of both Pax8‑AS1 and miR‑4646 in patients with GDM as compared with those in the healthy controls. In the second trimester, a strong negative correlation between Pax8‑AS1/miR‑4646 and 2‑h glucose levels was detected in patients with GDM. Receiver operating characteristic curve analysis indicated that the levels of Pax8‑AS1 and miR‑4646 in the second trimester of pregnancy had a significant diagnostic value with high selectivity and specificity for GDM (area under the curve values, 0.902 and 0.891, respectively; P<0.001). Overall, the present study suggested that Pax8‑AS1 and miR‑4646 may serve as promising diagnostic biomarkers for GDM.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

View References

1 

Binder AM, LaRocca J, Lesseur C, Marsit CJ and Michels KB: Epigenome-wide and transcriptome-wide analyses reveal gestational diabetes is associated with alterations in the human leukocyte antigen complex. Clin Epigenetics. 7(79)2015.PubMed/NCBI View Article : Google Scholar

2 

Zhu Y and Zhang C: Prevalence of gestational diabetes and risk of progression to type 2 diabetes: A global perspective. Curr Diab Rep. 16(7)2016.PubMed/NCBI View Article : Google Scholar

3 

International Association of Diabetes and Pregnancy Study Groups Consensus Panel. Metzger BE, Gabbe SG, Persson B, Buchanan TA, Catalano PA, Damm P, Dyer AR, Leiva A, Hod M, et al: International association of diabetes and pregnancy study groups recommendations on the diagnosis and classification of hyperglycemia in pregnancy. Diabetes Care. 33:676–682. 2010.PubMed/NCBI View Article : Google Scholar

4 

Sobngwi E, Boudou P, Mauvais-Jarvis F, Leblanc H, Velho G, Vexiau P, Porcher R, Hadjadj S, Pratley R, Tataranni PA, et al: Effect of a diabetic environment in utero on predisposition to type 2 diabetes. Lancet. 361:1861–1865. 2003.PubMed/NCBI View Article : Google Scholar

5 

Catalano PM and Hauguel-De Mouzon S: Is it time to revisit the Pedersen hypothesis in the face of the obesity epidemic? Am J Obstet Gynecol. 204:479–487. 2011.PubMed/NCBI View Article : Google Scholar

6 

El Hajj N, Schneider E, Lehnen H and Haaf T: Epigenetics and life-long consequences of an adverse nutritional and diabetic intrauterine environment. Reproduction. 148:R111–R120. 2014.PubMed/NCBI View Article : Google Scholar

7 

Esteller M: Non-coding RNAs in human disease. Nat Rev Genet. 12:861–874. 2011.PubMed/NCBI View Article : Google Scholar

8 

Anastasiadou E, Jacob LS and Slack FJ: Non-coding RNA networks in cancer. Nat Rev Cancer. 18:5–18. 2018.PubMed/NCBI View Article : Google Scholar

9 

Quinn JJ and Chang HY: Unique features of long non-coding RNA biogenesis and function. Nat Rev Genet. 17:47–62. 2016.PubMed/NCBI View Article : Google Scholar

10 

Yan C, Chen J and Chen N: Long noncoding RNA MALAT1 promotes hepatic steatosis and insulin resistance by increasing nuclear SREBP-1c protein stability. Sci Rep. 6(22640)2016.PubMed/NCBI View Article : Google Scholar

11 

Leng L, Zhang C, Ren L and Li Q: Construction of a long non-coding RNA-mediated competitive endogenous RNA network reveals global patterns and regulatory markers in gestational diabetes. Int J Mol Med. 43:927–935. 2019.PubMed/NCBI View Article : Google Scholar

12 

Tuna M, Machado AS and Calin GA: Genetic and epigenetic alterations of microRNAs and implications for human cancers and other diseases. Genes Chromosomes Cancer. 55:193–214. 2016.PubMed/NCBI View Article : Google Scholar

13 

Mitchell PS, Parkin RK, Kroh EM, Fritz BR, Wyman SK, Pogosova-Agadjanyan EL, Peterson A, Noteboom J, O'Briant KC, Allen A, et al: Circulating microRNAs as stable blood-based markers for cancer detection. Proc Natl Acad Sci USA. 105:10513–10518. 2008.PubMed/NCBI View Article : Google Scholar

14 

Esteves JV, Enguita FJ and Machado UF: MicroRNAs-mediated regulation of skeletal muscle GLUT4 expression and translocation in insulin resistance. J Diabetes Res. 2017(7267910)2017.PubMed/NCBI View Article : Google Scholar

15 

Pordzik J, Jakubik D, Jarosz-Popek J, Wicik Z, Eyileten C, De Rosa S, Indolfi C, Siller-Matula JM, Czajka P and Postula M: Significance of circulating microRNAs in diabetes mellitus type 2 and platelet reactivity: Bioinformatic analysis and review. Cardiovasc Diabetol. 18(113)2019.PubMed/NCBI View Article : Google Scholar

16 

Stirm L, Huypens P, Sass S, Batra R, Fritsche L, Brucker S, Abele H, Hennige AM, Theis F, Beckers J, et al: Maternal whole blood cell miRNA-340 is elevated in gestational diabetes and inversely regulated by glucose and insulin. Sci Rep. 8(1366)2018.PubMed/NCBI View Article : Google Scholar

17 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Method. 25:402–408. 2001.PubMed/NCBI View Article : Google Scholar

18 

Iempridee T, Wiwithaphon S, Piboonprai K, Pratedrat P, Khumkhrong P, Japrung D, Temisak S, Laiwejpithaya S, Chaopotong P and Dharakul T: Identification of reference genes for circulating long noncoding RNA analysis in serum of cervical cancer patients. FEBS Open Bio. 8:1844–1854. 2018.PubMed/NCBI View Article : Google Scholar

19 

Li C, Chen J and Qin G: Partial Youden index and its inferences. J Biopharm Stat. 29:385–399. 2019.PubMed/NCBI View Article : Google Scholar

20 

Wapinski O and Chang HY: Long noncoding RNAs and human disease. Trends Cell Biol. 21:354–361. 2011.PubMed/NCBI View Article : Google Scholar

21 

Li CH and Chen Y: Targeting long non-coding RNAs in cancers: Progress and prospects. Int J Biochem Cell Biol. 45:1895–1910. 2013.PubMed/NCBI View Article : Google Scholar

22 

Carter G, Miladinovic B, Patel AA, Deland L, Mastorides S and Patel NA: Circulating long noncoding RNA GAS5 levels are correlated to prevalence of type 2 diabetes mellitus. BBA Clin. 4:102–107. 2015.PubMed/NCBI View Article : Google Scholar

23 

Bahari G, Hashemi M, Naderi M, Sadeghi-Bojd S and Taheri M: Long non-coding RNA PAX8-AS1 polymorphisms increase the risk of childhood acute lymphoblastic leukemia. Biomed Rep. 8:184–190. 2018.PubMed/NCBI View Article : Google Scholar

24 

Han J, Zhou W, Jia M, Wen J, Jiang J, Shi J, Zhang K, Ma H, Liu J, Ren J, et al: Expression quantitative trait loci in long non-coding RNA PAX8-AS1 are associated with decreased risk of cervical cancer. Mol Genet Genomics. 291:1743–1748. 2016.PubMed/NCBI View Article : Google Scholar

25 

Zhang Y, Li F and Chen J: MYC promotes the development of papillary thyroid carcinoma by inhibiting the expression of lncRNA PAX8-AS1:28. Oncol Rep. 41:2511–2517. 2019.PubMed/NCBI View Article : Google Scholar

26 

Arner P and Kulyté A: MicroRNA regulatory networks in human adipose tissue and obesity. Nat Rev Endocrinol. 11:276–288. 2015.PubMed/NCBI View Article : Google Scholar

27 

Miao C, Chang J, Zhang G and Fang Y: MicroRNAs in type 1 diabetes: New research progress and potential directions. Biochem Cell Biol. 96:498–506. 2018.PubMed/NCBI View Article : Google Scholar

28 

Vienberg S, Geiger J, Madsen S and Dalgaard LT: MicroRNAs in metabolism. Acta Physiol (Oxf). 219:346–361. 2017.PubMed/NCBI View Article : Google Scholar

29 

Anton L, Olarerin-George AO, Hogenesch JB and Elovitz MA: Placental expression of miR-517a/b and miR-517c contributes to trophoblast dysfunction and preeclampsia. PLoS One. 10(e0122707)2015.PubMed/NCBI View Article : Google Scholar

30 

Wander PL, Boyko EJ, Hevner K, Parikh VJ, Tadesse MG, Sorensen TK, Williams MA and Enquobahrie DA: Circulating early- and mid-pregnancy microRNAs and risk of gestational diabetes. Diabetes Res Clin Pract. 132:1–9. 2017.PubMed/NCBI View Article : Google Scholar

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Hu J, Mu H, Gao L, Pan Y, Wu C, Zhang D, Chen Q and Ding H: Diagnostic value of candidate noncoding RNAs in leukocytes of patients with gestational diabetes mellitus. Exp Ther Med 21: 145, 2021.
APA
Hu, J., Mu, H., Gao, L., Pan, Y., Wu, C., Zhang, D. ... Ding, H. (2021). Diagnostic value of candidate noncoding RNAs in leukocytes of patients with gestational diabetes mellitus. Experimental and Therapeutic Medicine, 21, 145. https://doi.org/10.3892/etm.2020.9576
MLA
Hu, J., Mu, H., Gao, L., Pan, Y., Wu, C., Zhang, D., Chen, Q., Ding, H."Diagnostic value of candidate noncoding RNAs in leukocytes of patients with gestational diabetes mellitus". Experimental and Therapeutic Medicine 21.2 (2021): 145.
Chicago
Hu, J., Mu, H., Gao, L., Pan, Y., Wu, C., Zhang, D., Chen, Q., Ding, H."Diagnostic value of candidate noncoding RNAs in leukocytes of patients with gestational diabetes mellitus". Experimental and Therapeutic Medicine 21, no. 2 (2021): 145. https://doi.org/10.3892/etm.2020.9576
Copy and paste a formatted citation
x
Spandidos Publications style
Hu J, Mu H, Gao L, Pan Y, Wu C, Zhang D, Chen Q and Ding H: Diagnostic value of candidate noncoding RNAs in leukocytes of patients with gestational diabetes mellitus. Exp Ther Med 21: 145, 2021.
APA
Hu, J., Mu, H., Gao, L., Pan, Y., Wu, C., Zhang, D. ... Ding, H. (2021). Diagnostic value of candidate noncoding RNAs in leukocytes of patients with gestational diabetes mellitus. Experimental and Therapeutic Medicine, 21, 145. https://doi.org/10.3892/etm.2020.9576
MLA
Hu, J., Mu, H., Gao, L., Pan, Y., Wu, C., Zhang, D., Chen, Q., Ding, H."Diagnostic value of candidate noncoding RNAs in leukocytes of patients with gestational diabetes mellitus". Experimental and Therapeutic Medicine 21.2 (2021): 145.
Chicago
Hu, J., Mu, H., Gao, L., Pan, Y., Wu, C., Zhang, D., Chen, Q., Ding, H."Diagnostic value of candidate noncoding RNAs in leukocytes of patients with gestational diabetes mellitus". Experimental and Therapeutic Medicine 21, no. 2 (2021): 145. https://doi.org/10.3892/etm.2020.9576
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team