Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Experimental and Therapeutic Medicine
Join Editorial Board Propose a Special Issue
Print ISSN: 1792-0981 Online ISSN: 1792-1015
Journal Cover
February-2021 Volume 21 Issue 2

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
February-2021 Volume 21 Issue 2

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article Open Access

Influence of aging on the peripheral nerve repair process using an artificial nerve conduit

  • Authors:
    • Ayaka Kaneko
    • Kiyohito Naito
    • Shinji Nakamura
    • Katsumi Miyahara
    • Kenji Goto
    • Hiroyuki Obata
    • Nana Nagura
    • Yoichi Sugiyama
    • Kazuo Kaneko
    • Muneaki Ishijima
  • View Affiliations / Copyright

    Affiliations: Department of Orthopaedics, Juntendo University School of Medicine, Bunkyo, Tokyo 113‑8421, Japan, Laboratory of Morphology and Image Analysis, Research Support Center, Juntendo University Graduate School of Medicine, Bunkyo, Tokyo 113‑8421, Japan
    Copyright: © Kaneko et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 168
    |
    Published online on: December 21, 2020
       https://doi.org/10.3892/etm.2020.9599
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

The influence of aging on the induction of nerve regeneration in artificial nerve conduits has yet to be clarified. In the present study, artificial nerve conduit transplantation and histological analysis using the sciatic nerve of young and elderly mice were performed. Using 20 male C57BL/6 mice, an artificial nerve conduit was transplanted to the sciatic nerve at 8 weeks (Young group) or 70 weeks of age (Aged group), and the sciatic nerve was evaluated histologically at 1, 4 and 12 weeks after surgery. Using hematoxylin and eosin staining, the state of induction of nerve regeneration in the artificial nerve conduit was evaluated. Additionally, immunohistochemical staining was used to investigate an angiogenic marker [vascular endothelial growth factor A (VEGFA)], Schwann cell markers [sex determining region Y‑box 10 (SOX10) and S100 calcium‑binding protein β (S100β)] and a nerve damage marker [nerve growth factor (NGF)]. The results revealed that the induction of nerve regeneration was significantly higher in the Young group than in the Aged group. In addition, VEGFA and SOX10 expression at 1 week, SOX10 expression at 4 weeks and SOX10, S100β and NGF expression at 12 weeks in the proximal stump were significantly higher in the Young group than in the Aged group. At the center of the artificial nerve conduit, S100β and NGF expression at 4 weeks, and VEGFA, SOX10, S100β and NGF expression at 12 weeks were significantly higher in the Young group than in the Aged group. In the distal stump, no significant difference was noted in immunostaining at any week between the two groups. The present study suggested that the nerve regeneration‑inducing functions decrease due to aging.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

Figure 6

Figure 7

View References

1 

Iijima Y, Ajiki T, Murayama A and Takeshita K: Effect of artificial nerve conduit vascularization on peripheral nerve in a necrotic bed. Plast Reconstr Surg Glob Open. 4(e665)2016.PubMed/NCBI View Article : Google Scholar

2 

Kornfeld T, Vogt PM and Radtke C: Nerve grafting for peripheral nerve injuries with extended defect sizes. Wien Med Wochenschr. 169:240–251. 2019.PubMed/NCBI View Article : Google Scholar

3 

Rbia N, Bulstra LF, Saffari TM, Hovius SER and Shin AY: Collagen nerve conduits and processed nerve allografts for the reconstruction of digital nerve gaps: A single-institution case series and review of the literature. World Neurosurg. 127:e1176–e1184. 2019.PubMed/NCBI View Article : Google Scholar

4 

Siemionow M, Cwykiel J, Uygur S, Kwiecien G, Oztürk C, Szopinski J and Madajka M: Application of epineural sheath conduit for restoration of 6-cm long nerve defects in a sheep median nerve model. Microsurgery. 39:332–339. 2018.PubMed/NCBI View Article : Google Scholar

5 

Biasibetti E, Bisanzio D, Mioletti S, Amedeo S, Iuliano A, Bianco P and Capucchio MT: Spontaneous age-related changes of peripheral nerves in cattle: Morphological and biochemical studies. Anat Histol Embryol. 45:100–108. 2015.PubMed/NCBI View Article : Google Scholar

6 

Goto K, Naito K, Nakamura S, Nagura N, Sugiyama Y, Obata H, Kaneko A and Kaneko K: Protective mechanism against age-associated changes in the peripheral nerves. Life Sci. 253(117744)2020.PubMed/NCBI View Article : Google Scholar

7 

Novak CB, Anastakis DJ, Beaton DE, Mackinnon SE and Katz J: Biomedical and psychosocial factors associated with disability after peripheral nerve injury. J Bone Joint Surg Am. 93:929–936. 2011.PubMed/NCBI View Article : Google Scholar

8 

Fields RD, Le Beau JM, Longo FM and Ellisman MH: Nerve regeneration through artificial tubular implants. Prog Neurobiol. 33:87–134. 1989.PubMed/NCBI View Article : Google Scholar

9 

Painter MW, Lutz AB, Cheng YC, Latremoliere A, Duong K, Miller CM, Posada S, Cobos EJ, Zhang AX, Wagers AJ, et al: Diminished schwann cell repair responses underlie age-associated impaired axonal regeneration. Neuron. 83:331–343. 2014.PubMed/NCBI View Article : Google Scholar

10 

Verdú E, Ceballos D, Vilches JJ and Navarro X: Influence of aging on peripheral nerve function and regeneration. J Peripehr Nerv Syst. 5:191–208. 2000.PubMed/NCBI View Article : Google Scholar

11 

Pola R, Aprahamian TR, Bosch-Marcé M, Curry C, Gaetani E, Flex A, Smith RC, Isner JM and Losordo DW: Age-dependent VEGF expression and intraneural neovascularization during regeneration of peripheral nerves. Neurobiol Aging. 25:1361–1368. 2004.PubMed/NCBI View Article : Google Scholar

12 

Cattin AL, Burden JJ, Van Emmenis L, Mackenzie FE, Hoving JJ, Garcia Calavia N, Guo Y, McLaughlin M, Rosenberg LH, Quereda V, et al: Macrophage-induced blood vessels guide Schwann cell-mediated regeneration of peripheral nerves. Cell. 162:1127–1139. 2015.PubMed/NCBI View Article : Google Scholar

13 

Nishida Y, Yamada Y, Kanemaru H, Ohazama A, Maeda T and Seo K: Vascularization via activation of VEGF-VEGFR signaling is essential for peripheral nerve regeneration. Biomed Res. 39:287–294. 2018.PubMed/NCBI View Article : Google Scholar

14 

Fujiwara S, Hoshikawa S, Ueno T, Hirata M, Saito T, Ikeda T, Kawaguchi H, Nakamura K, Tanaka S and Ogata T: SOX10 transactivates S100B to suppress Schwann cell proliferation and to promote myelination. PLoS One. 9(e115400)2014.PubMed/NCBI View Article : Google Scholar

15 

Jessen KR and Mirsky R: Signals that determine Schwann cell identity. J Anat. 200:367–376. 2002.PubMed/NCBI View Article : Google Scholar

16 

Kuhlbrodt K, Herbarth B, Sock E, Hermans-Borgmeyer I and Wegner M: Sox10, a novel transcriptional modulator in glial cells. J Neurosci. 18:237–250. 1998.PubMed/NCBI View Article : Google Scholar

17 

Budni J, Bellettini-Santos T, Mina F, Garcez ML and Zugno AI: The involvement of BDNF, NGF and GDNF in aging and Alzheimer's disease. Aging Dis. 6:331–341. 2015.PubMed/NCBI View Article : Google Scholar

18 

Chen ZW and Wang MS: Effects of nerve growth factor on crushed sciatic nerve regeneration in rats. Microsurgery. 16:547–551. 1995.PubMed/NCBI View Article : Google Scholar

19 

Hasenöhrl RU, Söderstróm S, Mohammed AH, Ebendal T and Huston JP: Reciprocal changes in expression of mRNA for nerve growth factor and its receptors TrkA and LNGFR in brain of aged rats in relation to maze learning deficits. Exp Brain Res. 114:205–213. 1997.PubMed/NCBI View Article : Google Scholar

20 

Kemp SW, Webb AA, Dhaliwal S, Syed S, Walsh SK and Midha R: Dose and duration of nerve growth factor (NGF) administration determine the extent of behavioral recovery following peripheral nerve injury in the rat. Exp Neurol. 229:460–470. 2011.PubMed/NCBI View Article : Google Scholar

21 

Lärkfors L, Ebendal T, Whittemore SR, Persson H, Hoffer B and Olson L: Decreased level of nerve growth factor (NGF) and its messenger RNA in the aged rat brain. Brain Res. 427:55–60. 1987.PubMed/NCBI View Article : Google Scholar

22 

Grinsell D and Keating CP: Peripheral nerve reconstruction after injury: A review of clinical and experimental therapies. Biomed Res Int. 2014(698256)2014.PubMed/NCBI View Article : Google Scholar

23 

Hall S: Nerve repair: A neurobiologist's view. J Hand Surg Br. 26:129–136. 2001.PubMed/NCBI View Article : Google Scholar

24 

Mokuno K, Sobue G, Reddy UR, Wurzer J, Kreider B, Hotta H, Baron P, Ross AH and Pleasure D: Regulation of Schwann cell nerve growth factor receptor by cyclic adenosine 3',5'-monophosphate. J Neurosci Res. 21:465–472. 1988.PubMed/NCBI View Article : Google Scholar

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Kaneko A, Naito K, Nakamura S, Miyahara K, Goto K, Obata H, Nagura N, Sugiyama Y, Kaneko K, Ishijima M, Ishijima M, et al: Influence of aging on the peripheral nerve repair process using an artificial nerve conduit. Exp Ther Med 21: 168, 2021.
APA
Kaneko, A., Naito, K., Nakamura, S., Miyahara, K., Goto, K., Obata, H. ... Ishijima, M. (2021). Influence of aging on the peripheral nerve repair process using an artificial nerve conduit. Experimental and Therapeutic Medicine, 21, 168. https://doi.org/10.3892/etm.2020.9599
MLA
Kaneko, A., Naito, K., Nakamura, S., Miyahara, K., Goto, K., Obata, H., Nagura, N., Sugiyama, Y., Kaneko, K., Ishijima, M."Influence of aging on the peripheral nerve repair process using an artificial nerve conduit". Experimental and Therapeutic Medicine 21.2 (2021): 168.
Chicago
Kaneko, A., Naito, K., Nakamura, S., Miyahara, K., Goto, K., Obata, H., Nagura, N., Sugiyama, Y., Kaneko, K., Ishijima, M."Influence of aging on the peripheral nerve repair process using an artificial nerve conduit". Experimental and Therapeutic Medicine 21, no. 2 (2021): 168. https://doi.org/10.3892/etm.2020.9599
Copy and paste a formatted citation
x
Spandidos Publications style
Kaneko A, Naito K, Nakamura S, Miyahara K, Goto K, Obata H, Nagura N, Sugiyama Y, Kaneko K, Ishijima M, Ishijima M, et al: Influence of aging on the peripheral nerve repair process using an artificial nerve conduit. Exp Ther Med 21: 168, 2021.
APA
Kaneko, A., Naito, K., Nakamura, S., Miyahara, K., Goto, K., Obata, H. ... Ishijima, M. (2021). Influence of aging on the peripheral nerve repair process using an artificial nerve conduit. Experimental and Therapeutic Medicine, 21, 168. https://doi.org/10.3892/etm.2020.9599
MLA
Kaneko, A., Naito, K., Nakamura, S., Miyahara, K., Goto, K., Obata, H., Nagura, N., Sugiyama, Y., Kaneko, K., Ishijima, M."Influence of aging on the peripheral nerve repair process using an artificial nerve conduit". Experimental and Therapeutic Medicine 21.2 (2021): 168.
Chicago
Kaneko, A., Naito, K., Nakamura, S., Miyahara, K., Goto, K., Obata, H., Nagura, N., Sugiyama, Y., Kaneko, K., Ishijima, M."Influence of aging on the peripheral nerve repair process using an artificial nerve conduit". Experimental and Therapeutic Medicine 21, no. 2 (2021): 168. https://doi.org/10.3892/etm.2020.9599
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team