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TNF‑α promotes insulin resistance in obstructive sleep apnea‑hypopnea syndrome

  • Authors:
    • Lin Zhao
    • Yang Liu
    • Xiangrong Wang
  • View Affiliations / Copyright

    Affiliations: Department of Endocrinology, The 8th Medical Center of Chinese People's Liberation Army General Hospital, Beijing 100091, P.R. China, Department of Nursing, Jiangsu Union Technical Institute Nantong Health Branch, Nantong, Jiangsu 226010, P.R. China
    Copyright: © Zhao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 568
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    Published online on: March 29, 2021
       https://doi.org/10.3892/etm.2021.10000
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Abstract

Obstructive sleep apnea hypopnea syndrome (OSAHS) is the most serious among children with sleep disordered breathing. The present study aimed to investigate whether TNF‑α could decrease the glucose transporter type 4 insulin‑responsive (GLUT‑4) expression to promote insulin resistance through the TNF‑α/IKKβ/IKβ/NF‑κB signaling pathway in OSAHS. In total, 30 obese children with OSAHS and 30 non‑OSAHS obese children were enrolled into the present study. TNF‑α expression in adenoid tissues was detected by western blot analysis and immunohistochemistry. The expression of inflammatory factors (IL‑1β, IL‑6 and IFN‑γ) and TNF‑α/IKKβ/IKβ/NF‑κB signaling pathway‑associated proteins was also detected by western blot analysis. The expression of insulin resistance‑associated factors, insulin receptor substrate 1 (IRS1) and GLUT4, was determined by western blot analysis and immunohistochemistry. TNF‑α expression was increased in adenoid tissues of children with OSAHS, which was also confirmed by immunohistochemistry. The expression levels of IL‑1β, IL‑6 and IFN‑γ were all upregulated in adenoid tissues of children with OSAHS. The expression of IRS1 and GLUT4 was decreased in adenoid tissues of obese children with OSAHS and the result of immunohistochemistry was consistent with the result of western blot analysis. The protein level of TNF‑α, and ratio of phosphorylated (p‑)/total (t)‑IKKβ, p/t‑IKβ and p/t‑NF‑κB was increased in adenoid tissues of children with OSAHS. TNF‑α could suppress the GLUT4 expression to promote insulin resistance by TNF‑α/IKKβ/IKβ/NF‑κB signaling pathway in OSAHS.
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Copy and paste a formatted citation
Spandidos Publications style
Zhao L, Liu Y and Wang X: TNF‑α promotes insulin resistance in obstructive sleep apnea‑hypopnea syndrome. Exp Ther Med 21: 568, 2021.
APA
Zhao, L., Liu, Y., & Wang, X. (2021). TNF‑α promotes insulin resistance in obstructive sleep apnea‑hypopnea syndrome. Experimental and Therapeutic Medicine, 21, 568. https://doi.org/10.3892/etm.2021.10000
MLA
Zhao, L., Liu, Y., Wang, X."TNF‑α promotes insulin resistance in obstructive sleep apnea‑hypopnea syndrome". Experimental and Therapeutic Medicine 21.6 (2021): 568.
Chicago
Zhao, L., Liu, Y., Wang, X."TNF‑α promotes insulin resistance in obstructive sleep apnea‑hypopnea syndrome". Experimental and Therapeutic Medicine 21, no. 6 (2021): 568. https://doi.org/10.3892/etm.2021.10000
Copy and paste a formatted citation
x
Spandidos Publications style
Zhao L, Liu Y and Wang X: TNF‑α promotes insulin resistance in obstructive sleep apnea‑hypopnea syndrome. Exp Ther Med 21: 568, 2021.
APA
Zhao, L., Liu, Y., & Wang, X. (2021). TNF‑α promotes insulin resistance in obstructive sleep apnea‑hypopnea syndrome. Experimental and Therapeutic Medicine, 21, 568. https://doi.org/10.3892/etm.2021.10000
MLA
Zhao, L., Liu, Y., Wang, X."TNF‑α promotes insulin resistance in obstructive sleep apnea‑hypopnea syndrome". Experimental and Therapeutic Medicine 21.6 (2021): 568.
Chicago
Zhao, L., Liu, Y., Wang, X."TNF‑α promotes insulin resistance in obstructive sleep apnea‑hypopnea syndrome". Experimental and Therapeutic Medicine 21, no. 6 (2021): 568. https://doi.org/10.3892/etm.2021.10000
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