Open Access

The herbal agent plantamajoside, exerts a potential inhibitory effect on the development of hepatocellular carcinoma

  • Authors:
    • Shu Luo
    • Xing Jiang
    • Gang Yin
    • Yajun Liu
    • Zhou Liu
    • Linglian Meng
    • Jian Wu
    • Haoxin Wu
  • View Affiliations

  • Published online on: March 31, 2021     https://doi.org/10.3892/etm.2021.10005
  • Article Number: 573
  • Copyright: © Luo et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Plantamajoside (PMS), a major component of Plantago asiatica L, has several pharmacological properties, including anti‑proliferative, anti‑inflammatory and anti‑tumor effects. However, the effects of PMS on hepatocellular carcinoma (HCC) have yet to be determined. The aim of the present study was to investigate the effects of PMS on HCC and elucidate the underlying mechanism. All assays were conducted using 5 groups, namely control, sorafenib, and PMS 100, 50, and 25 µg/ml groups. Cell proliferation was determined by the MTT assay. Cell migration was evaluated with the wound healing and Transwell assays, respectively. Cell apoptosis and cell cycle distribution were evaluated via flow cytometry. Reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR) analysis and western blotting were used to further investigate the mechanism of action of PMS. Sorafenib and PMS both significantly attenuated the proliferation and migration of HCC cells, and markedly promoted cell apoptosis. PMS induced cell cycle arrest in the G0/G1 phase. The efficacy of PMS increased in a dose‑dependent manner. Further study evaluated the expression of peroxisome proliferator‑activated receptor (PPARγ), nuclear factor (NF)‑κB and cyclooxygenase (Cox‑2) using RT‑qPCR analysis and western blotting. The results demonstrated that PMS promoted the expression of PPARγ and suppressed the expression of NF‑κB and Cox‑2. In conclusion, PMS was shown to affect cell proliferation, migration, apoptosis and cell cycle distribution. Furthermore, PMS promoted the expression of PPARγ and inhibited the expression of NF‑κB and Cox‑2, which may be the mechanism underlying its biological effects. Based on the results of the present study, PMS appears to be a promising agent for HCC therapy.
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June-2021
Volume 21 Issue 6

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Luo S, Jiang X, Yin G, Liu Y, Liu Z, Meng L, Wu J and Wu H: The herbal agent plantamajoside, exerts a potential inhibitory effect on the development of hepatocellular carcinoma. Exp Ther Med 21: 573, 2021
APA
Luo, S., Jiang, X., Yin, G., Liu, Y., Liu, Z., Meng, L. ... Wu, H. (2021). The herbal agent plantamajoside, exerts a potential inhibitory effect on the development of hepatocellular carcinoma. Experimental and Therapeutic Medicine, 21, 573. https://doi.org/10.3892/etm.2021.10005
MLA
Luo, S., Jiang, X., Yin, G., Liu, Y., Liu, Z., Meng, L., Wu, J., Wu, H."The herbal agent plantamajoside, exerts a potential inhibitory effect on the development of hepatocellular carcinoma". Experimental and Therapeutic Medicine 21.6 (2021): 573.
Chicago
Luo, S., Jiang, X., Yin, G., Liu, Y., Liu, Z., Meng, L., Wu, J., Wu, H."The herbal agent plantamajoside, exerts a potential inhibitory effect on the development of hepatocellular carcinoma". Experimental and Therapeutic Medicine 21, no. 6 (2021): 573. https://doi.org/10.3892/etm.2021.10005