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Interferon‑τ regulates the expression and function of bovine leukocyte antigen by downregulating bta‑miR‑204

  • Authors:
    • Xiaoyan Wang
    • Ting Yuan
    • Nannan Yin
    • Xiaofei Ma
    • Yaping Yang
    • Jing Yang
    • Aftab Shaukat
    • Ganzhen Deng
  • View Affiliations / Copyright

    Affiliations: Department of Clinical Veterinary Medicine, College of Animal Science and Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei 430070, P.R. China, College of Animal Science and Technology, Chongqing Three Gorges Vocational College, Chongqing 404155, P.R. China
    Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 594
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    Published online on: April 8, 2021
       https://doi.org/10.3892/etm.2021.10026
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Abstract

IFN‑τ is a pregnancy recognition factor that regulates embryo implantation in ruminants. IFN‑τ has been suggested to be involved in the expression of microRNA (miRNA/miR) and bovine leukocyte antigen (BoLA), which is an analog of the human major histocompatibility complex class I. However, little is known about whether the miRNAs are involved in the expression of BoLA in ruminants. The present study firstly verified that bta‑miR‑204 was downregulated and that BoLA was upregulated in the uterine tissues of dairy cows during early pregnancy. Subsequently, luciferase reporter assays, reverse transcription‑quantitative PCR and western blot analysis were used to validate BoLA as the target gene of bta‑miR‑204. Moreover, BoLA was markedly upregulated and bta‑miR‑204 was downregulated in bovine endometrial epithelial cells (bEECs) treated with IFN‑τ. In addition, the results indicated that when the expression level of BoLA was increased by IFN‑τ, the expression level of programmed death‑ligand 1 (PD‑L1) and programmed death‑ligand 2 (PD‑L2) was also increased. Furthermore, when BoLA was silenced in bEECs by small interfering RNA, the expression of PD‑L1 and PD‑L2 was not affected by IFN‑τ. The expression level of PD‑L1 and PD‑L2 was also increased in the uterine tissues of pregnant dairy cattle. In conclusion, IFN‑τ may function by suppressing the expression of bta‑miR‑204 to increase the expression of BoLA during the embryo implantation period in cattle. IFN‑τ may induce PD‑L1 and PD‑L2 transcription by regulating BoLA, which may influence the T cell immune response, thereby regulating pregnant cattle immunization.
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Spandidos Publications style
Wang X, Yuan T, Yin N, Ma X, Yang Y, Yang J, Shaukat A and Deng G: Interferon‑τ regulates the expression and function of bovine leukocyte antigen by downregulating bta‑miR‑204. Exp Ther Med 21: 594, 2021.
APA
Wang, X., Yuan, T., Yin, N., Ma, X., Yang, Y., Yang, J. ... Deng, G. (2021). Interferon‑τ regulates the expression and function of bovine leukocyte antigen by downregulating bta‑miR‑204. Experimental and Therapeutic Medicine, 21, 594. https://doi.org/10.3892/etm.2021.10026
MLA
Wang, X., Yuan, T., Yin, N., Ma, X., Yang, Y., Yang, J., Shaukat, A., Deng, G."Interferon‑τ regulates the expression and function of bovine leukocyte antigen by downregulating bta‑miR‑204". Experimental and Therapeutic Medicine 21.6 (2021): 594.
Chicago
Wang, X., Yuan, T., Yin, N., Ma, X., Yang, Y., Yang, J., Shaukat, A., Deng, G."Interferon‑τ regulates the expression and function of bovine leukocyte antigen by downregulating bta‑miR‑204". Experimental and Therapeutic Medicine 21, no. 6 (2021): 594. https://doi.org/10.3892/etm.2021.10026
Copy and paste a formatted citation
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Spandidos Publications style
Wang X, Yuan T, Yin N, Ma X, Yang Y, Yang J, Shaukat A and Deng G: Interferon‑τ regulates the expression and function of bovine leukocyte antigen by downregulating bta‑miR‑204. Exp Ther Med 21: 594, 2021.
APA
Wang, X., Yuan, T., Yin, N., Ma, X., Yang, Y., Yang, J. ... Deng, G. (2021). Interferon‑τ regulates the expression and function of bovine leukocyte antigen by downregulating bta‑miR‑204. Experimental and Therapeutic Medicine, 21, 594. https://doi.org/10.3892/etm.2021.10026
MLA
Wang, X., Yuan, T., Yin, N., Ma, X., Yang, Y., Yang, J., Shaukat, A., Deng, G."Interferon‑τ regulates the expression and function of bovine leukocyte antigen by downregulating bta‑miR‑204". Experimental and Therapeutic Medicine 21.6 (2021): 594.
Chicago
Wang, X., Yuan, T., Yin, N., Ma, X., Yang, Y., Yang, J., Shaukat, A., Deng, G."Interferon‑τ regulates the expression and function of bovine leukocyte antigen by downregulating bta‑miR‑204". Experimental and Therapeutic Medicine 21, no. 6 (2021): 594. https://doi.org/10.3892/etm.2021.10026
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