Open Access

Interferon‑τ regulates the expression and function of bovine leukocyte antigen by downregulating bta‑miR‑204

  • Authors:
    • Xiaoyan Wang
    • Ting Yuan
    • Nannan Yin
    • Xiaofei Ma
    • Yaping Yang
    • Jing Yang
    • Aftab Shaukat
    • Ganzhen Deng
  • View Affiliations

  • Published online on: April 8, 2021     https://doi.org/10.3892/etm.2021.10026
  • Article Number: 594
  • Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

IFN‑τ is a pregnancy recognition factor that regulates embryo implantation in ruminants. IFN‑τ has been suggested to be involved in the expression of microRNA (miRNA/miR) and bovine leukocyte antigen (BoLA), which is an analog of the human major histocompatibility complex class I. However, little is known about whether the miRNAs are involved in the expression of BoLA in ruminants. The present study firstly verified that bta‑miR‑204 was downregulated and that BoLA was upregulated in the uterine tissues of dairy cows during early pregnancy. Subsequently, luciferase reporter assays, reverse transcription‑quantitative PCR and western blot analysis were used to validate BoLA as the target gene of bta‑miR‑204. Moreover, BoLA was markedly upregulated and bta‑miR‑204 was downregulated in bovine endometrial epithelial cells (bEECs) treated with IFN‑τ. In addition, the results indicated that when the expression level of BoLA was increased by IFN‑τ, the expression level of programmed death‑ligand 1 (PD‑L1) and programmed death‑ligand 2 (PD‑L2) was also increased. Furthermore, when BoLA was silenced in bEECs by small interfering RNA, the expression of PD‑L1 and PD‑L2 was not affected by IFN‑τ. The expression level of PD‑L1 and PD‑L2 was also increased in the uterine tissues of pregnant dairy cattle. In conclusion, IFN‑τ may function by suppressing the expression of bta‑miR‑204 to increase the expression of BoLA during the embryo implantation period in cattle. IFN‑τ may induce PD‑L1 and PD‑L2 transcription by regulating BoLA, which may influence the T cell immune response, thereby regulating pregnant cattle immunization.
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June-2021
Volume 21 Issue 6

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Wang X, Yuan T, Yin N, Ma X, Yang Y, Yang J, Shaukat A and Deng G: Interferon‑τ regulates the expression and function of bovine leukocyte antigen by downregulating bta‑miR‑204. Exp Ther Med 21: 594, 2021
APA
Wang, X., Yuan, T., Yin, N., Ma, X., Yang, Y., Yang, J. ... Deng, G. (2021). Interferon‑τ regulates the expression and function of bovine leukocyte antigen by downregulating bta‑miR‑204. Experimental and Therapeutic Medicine, 21, 594. https://doi.org/10.3892/etm.2021.10026
MLA
Wang, X., Yuan, T., Yin, N., Ma, X., Yang, Y., Yang, J., Shaukat, A., Deng, G."Interferon‑τ regulates the expression and function of bovine leukocyte antigen by downregulating bta‑miR‑204". Experimental and Therapeutic Medicine 21.6 (2021): 594.
Chicago
Wang, X., Yuan, T., Yin, N., Ma, X., Yang, Y., Yang, J., Shaukat, A., Deng, G."Interferon‑τ regulates the expression and function of bovine leukocyte antigen by downregulating bta‑miR‑204". Experimental and Therapeutic Medicine 21, no. 6 (2021): 594. https://doi.org/10.3892/etm.2021.10026