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Total flavonoids of Rhizoma Drynariae combined with calcium attenuate osteoporosis by reducing reactive oxygen species generation

  • Authors:
    • Panyun Mu
    • Yimei Hu
    • Xu Ma
    • Jingru Shi
    • Zhendong Zhong
    • Lingyuan Huang
  • View Affiliations / Copyright

    Affiliations: Department of Orthopedics, Hospital of Chengdu University of Traditional Chinese Medicine, Chengdu, Sichuan 610075, P.R. China, Laboratory Animal Research Institute of Sichuan Provincial People's Hospital, Chengdu, Sichuan 610072, P.R. China, Chengdu Lilai Biotechnology Co., Ltd., Chengdu, Sichuan 610041, P.R. China
    Copyright: © Mu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 618
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    Published online on: April 14, 2021
       https://doi.org/10.3892/etm.2021.10050
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Abstract

In the present study, the effects of total flavonoids of Rhizoma Drynariae (TFRD) and calcium carbonate (CaCO3) on osteoporosis (OP) were assessed in a rat model of OP. For this purpose, 36 Sprague‑Dawley rats, aged 3 months, were randomly divided into a group undergoing sham surgery (sham‑operated group), model group (OP group), CaCO3 group (OP + CaCO3 group), TFRD group (OP + TFRD group), TFRD combined with CaCO3 group (OP + TFRD + CaCO3 group) and TFRD and CaCO3 combined with N‑acetyl cysteine group (OP + TFRD + CaCO3 + NAC group). The rat model of OP was established by bilateral ovariectomy. The changes in bone mineral density (BMD), bone volume parameters and bone histopathology in the rats from each group were observed. The levels of serum reactive oxygen species, superoxide dismutase (SOD), malondialdehyde, glutathione peroxidase (GSH‑Px), interleukin (IL)‑6, IL‑1β, TNF‑α, and the levels of bone tissue runt‑related transcription factor 2 (RUNX2), osteoprotegerin (OPG), osteocalcin (BGP), PI3K, p‑PI3K, AKT, p‑AKT, mammalian target of rapamycin (mTOR) and p‑mTOR were measured in the rats of each group. The induction of OP was associated with a marked decrease in BMD, bone mineral content, bone volume fraction and trabecular thickness, and decreased serum levels of SOD and GSH‑Px. Moreover, the expressions of RUNX2, OPG, BGP were downregulated and an upregulation of p‑PI3K, p‑AKT and p‑mTOR were observed in osteoporotic rats. However, treatment with TFRD and CaCO3 restored all the aforementioned parameters to almost normal values. Furthermore, the findings on histopathological evaluation were consistent with the biochemical observations. Taken together, the findings of the present study demonstrated that TFRD and CaCO3 significantly increased the antioxidant capacity in rats with OP, increased BMD and reduced bone mineral loss, and may be useful for the prevention and treatment of OP.
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Copy and paste a formatted citation
Spandidos Publications style
Mu P, Hu Y, Ma X, Shi J, Zhong Z and Huang L: Total flavonoids of <em>Rhizoma Drynariae</em> combined with calcium attenuate osteoporosis by reducing reactive oxygen species generation. Exp Ther Med 21: 618, 2021.
APA
Mu, P., Hu, Y., Ma, X., Shi, J., Zhong, Z., & Huang, L. (2021). Total flavonoids of <em>Rhizoma Drynariae</em> combined with calcium attenuate osteoporosis by reducing reactive oxygen species generation. Experimental and Therapeutic Medicine, 21, 618. https://doi.org/10.3892/etm.2021.10050
MLA
Mu, P., Hu, Y., Ma, X., Shi, J., Zhong, Z., Huang, L."Total flavonoids of <em>Rhizoma Drynariae</em> combined with calcium attenuate osteoporosis by reducing reactive oxygen species generation". Experimental and Therapeutic Medicine 21.6 (2021): 618.
Chicago
Mu, P., Hu, Y., Ma, X., Shi, J., Zhong, Z., Huang, L."Total flavonoids of <em>Rhizoma Drynariae</em> combined with calcium attenuate osteoporosis by reducing reactive oxygen species generation". Experimental and Therapeutic Medicine 21, no. 6 (2021): 618. https://doi.org/10.3892/etm.2021.10050
Copy and paste a formatted citation
x
Spandidos Publications style
Mu P, Hu Y, Ma X, Shi J, Zhong Z and Huang L: Total flavonoids of <em>Rhizoma Drynariae</em> combined with calcium attenuate osteoporosis by reducing reactive oxygen species generation. Exp Ther Med 21: 618, 2021.
APA
Mu, P., Hu, Y., Ma, X., Shi, J., Zhong, Z., & Huang, L. (2021). Total flavonoids of <em>Rhizoma Drynariae</em> combined with calcium attenuate osteoporosis by reducing reactive oxygen species generation. Experimental and Therapeutic Medicine, 21, 618. https://doi.org/10.3892/etm.2021.10050
MLA
Mu, P., Hu, Y., Ma, X., Shi, J., Zhong, Z., Huang, L."Total flavonoids of <em>Rhizoma Drynariae</em> combined with calcium attenuate osteoporosis by reducing reactive oxygen species generation". Experimental and Therapeutic Medicine 21.6 (2021): 618.
Chicago
Mu, P., Hu, Y., Ma, X., Shi, J., Zhong, Z., Huang, L."Total flavonoids of <em>Rhizoma Drynariae</em> combined with calcium attenuate osteoporosis by reducing reactive oxygen species generation". Experimental and Therapeutic Medicine 21, no. 6 (2021): 618. https://doi.org/10.3892/etm.2021.10050
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