Open Access

Effects of hypothermia on inflammatory cytokine expression in rat liver following asphyxial cardiac arrest

  • Authors:
    • Yoonsoo Park
    • Ji Hyeon Ahn
    • Jeong Hwi Cho
    • Hyun-Jin Tae
    • Tae-Kyeong Lee
    • Bora Kim
    • Jae-Chul Lee
    • Joon Ha Park
    • Myoung Cheol Shin
    • Taek Geun Ohk
    • Jun Hwi Cho
    • Moo-Ho Won
  • View Affiliations

  • Published online on: April 15, 2021     https://doi.org/10.3892/etm.2021.10058
  • Article Number: 626
  • Copyright: © Park et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Hypothermic treatment is known to protect against cardiac arrest (CA) and improve survival rate. However, few studies have evaluated the CA‑induced liver damage and the effects of hypothermia on this damage. Therefore, the aim of the present study was to determine possible protective effects of hypothermia on the liver after asphyxial CA. Rats were subjected to a 5‑min asphyxial CA followed by return of spontaneous circulation (ROSC). The body temperature was controlled at 37±0.5˚C (normothermia group) or 33±0.5˚C (hypothermia group) for 4 h after ROSC. Livers were examined at 6, 12 h, 1 and 2 days after ROSC. Histopathological examination was performed by H&E staining. Alterations in the expression levels of pro‑inflammatory (TNF‑α and interleukin IL‑2) and anti‑inflammatory cytokines (IL‑4 and IL‑13) were investigated by immunohistochemistry. Sinusoidal dilatation and vacuolization were observed after asphyxial CA by histopathological examination. However, these CA‑induced structural alterations were prevented by hypothermia. In immunohistochemical examination, the expression levels of pro‑inflammatory cytokines were reduced in the hypothermia group compared with those in the normothermia group while the expression levels of anti‑inflammatory cytokines were increased in the hypothermia group compared with those in the normothermia group. In conclusion, hypothermic treatment for 4 h following asphyxial CA in rats inhibited the increase of pro‑inflammatory cytokines and stimulated the expression of anti‑inflammatory cytokines compared with the normothermic group. The results of the present study suggested that hypothermic treatment after asphyxial CA reduced liver damage via the regulation of inflammation.

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June-2021
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Spandidos Publications style
Park Y, Ahn JH, Cho JH, Tae H, Lee T, Kim B, Lee J, Park JH, Shin MC, Ohk TG, Ohk TG, et al: Effects of hypothermia on inflammatory cytokine expression in rat liver following asphyxial cardiac arrest. Exp Ther Med 21: 626, 2021
APA
Park, Y., Ahn, J.H., Cho, J.H., Tae, H., Lee, T., Kim, B. ... Won, M. (2021). Effects of hypothermia on inflammatory cytokine expression in rat liver following asphyxial cardiac arrest. Experimental and Therapeutic Medicine, 21, 626. https://doi.org/10.3892/etm.2021.10058
MLA
Park, Y., Ahn, J. H., Cho, J. H., Tae, H., Lee, T., Kim, B., Lee, J., Park, J. H., Shin, M. C., Ohk, T. G., Cho, J. H., Won, M."Effects of hypothermia on inflammatory cytokine expression in rat liver following asphyxial cardiac arrest". Experimental and Therapeutic Medicine 21.6 (2021): 626.
Chicago
Park, Y., Ahn, J. H., Cho, J. H., Tae, H., Lee, T., Kim, B., Lee, J., Park, J. H., Shin, M. C., Ohk, T. G., Cho, J. H., Won, M."Effects of hypothermia on inflammatory cytokine expression in rat liver following asphyxial cardiac arrest". Experimental and Therapeutic Medicine 21, no. 6 (2021): 626. https://doi.org/10.3892/etm.2021.10058