Differential proteomic analysis of tibial subchondral bone from male and female guinea pigs with spontaneous osteoarthritis

Wang,Ying; Wang,Ying; Wang,Ying; Wu,Chengai; Wu,Chengai; Wu,Chengai; Tao,Jianfeng; Tao,Jianfeng; Tao,Jianfeng; Zhao,Danhui; Zhao,Danhui; Zhao,Danhui; Jiang,Xu; Jiang,Xu; Jiang,Xu; Tian,Wei; Tian,Wei; Tian,Wei

doi:10.3892/etm.2021.10065

Differential proteomic analysis of tibial subchondral bone from male and female guinea pigs with spontaneous osteoarthritis

Authors:
- Ying Wang
- Chengai Wu
- Jianfeng Tao
- Danhui Zhao
- Xu Jiang
- Wei Tian
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Affiliations: Department of Molecular Orthopedics, Beijing Research Institute of Traumatology and Orthopedics, Beijing 100035, P.R. China, Animal Laboratory, Beijing Research Institute of Traumatology and Orthopedics, Beijing 100035, P.R. China, Department of Orthopedics, Beijing Jishuitan Hospital, Beijing 100035, P.R. China, Department of Spine Surgery, Beijing Jishuitan Hospital, Beijing 100035, P.R. China
Published online on: April 15, 2021 https://doi.org/10.3892/etm.2021.10065
Article Number: 633
Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

A proteomic study on the tibial subchondral bone in guinea pigs with spontaneous osteoarthritis was performed to investigate the molecular alterations that occur in early osteoarthritis. A total of 132 healthy Hartley guinea pigs (aged 1 month; 66 female and 66 male) were randomly divided into 11 groups of six. Changes in articular cartilage and tibial subchondral bone were assessed using macroscopic examinations and micro‑computed tomography. iTRAQ‑integrated liquid chromatography‑tandem mass spectrometry was used to identify differentially altered proteins in the tibial subchondral bone between 1‑ and 3‑month‑old guinea pigs, which were then validated using western blotting. A gradual progression of cartilage degeneration was observed in the knee joints of the subject animals from 5‑11 months. With aging, the tibial subchondral trabecular bone acquired more plate‑like and less anisotropic properties, with increased bone mineral density, bone volume, trabecular thickness and numbers. The proteomic study identified 138 and 113 proteins significantly differentially expressed between 3‑ and 1‑month old guinea pigs in both the male and female animals, respectively. Western blotting confirmed the increased expression of osteoblast‑associated protein S100 calcium‑binding protein A8 (S100A8) and the deregulated expression of osteoclast‑associated proteins coronin 1A (CORO1A) and T‑cell immune regulator 1 (TCIRG1) in the 3‑month old guinea pigs in comparison to the 1‑month old guinea pigs. Spontaneous cartilage degeneration in the knee joints of male Hartley guinea pigs tended to be more serious compared with the females during the development of osteoarthritis. Together, the results suggest that osteoblast‑associated protein S100A8 and osteoclast‑associated proteins CORO1A and TCIRG1 are potentially key regulators of early osteoarthritic development in tibial subchondral bone.

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