Upregulation of long non‑coding RNA MYU promotes proliferation, migration and invasion of esophageal squamous cell carcinoma cells

Gu,Shudong; Qian,Li; Liu,Yan; Miao,Jiefei; Shen,Hong; Zhang,Shu; Mao,Guoxin

doi:10.3892/etm.2021.10076

Upregulation of long non‑coding RNA MYU promotes proliferation, migration and invasion of esophageal squamous cell carcinoma cells

Authors:
- Shudong Gu
- Li Qian
- Yan Liu
- Jiefei Miao
- Hong Shen
- Shu Zhang
- Guoxin Mao
View Affiliations

Affiliations: Department of Oncology, Affiliated Hospital of Nantong University, Nantong, Jiangsu 226001, P.R. China, Department of Pathology, Affiliated Hospital of Nantong University, Nantong, Jiangsu 226001, P.R. China
Published online on: April 18, 2021 https://doi.org/10.3892/etm.2021.10076
Article Number: 644
Copyright: © Gu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Esophageal squamous cell carcinoma (ESCC) is a common malignant tumour type of the digestive system. Long non‑coding RNA (lncRNA) c‑Myc upregulated (MYU), also known as VPS9 domain‑containing 1 antisense 1, was recently discovered. However, the expression of lncRNA MYU in ESCC and its role in tumour progression have remained elusive. In the present study, the expression of lncRNA MYU, Ki‑67 and the epithelial‑mesenchymal transition‑related proteins E‑cadherin and Vimentin in ESCC tissues was detected by reverse transcription‑quantitative PCR. The expression of Ki‑67, E‑cadherin and Vimentin in ESCC tissues was also detected by immunohistochemistry. A small interfering RNA plasmid was employed to establish a TE‑2 cell line with knockdown on lncRNA MYU. The results indicated that the expression of lncRNA MYU was higher in ESCC tissues than in normal adjacent tissues and that upregulation of lncRNA MYU was a potential biomarker for poor prognosis. The results also suggested that the expression levels of lncRNA MYU were correlated with the histological grade, lymph node metastasis and TNM stage (P<0.05). Silencing of lncRNA MYU expression inhibited the proliferation, migration and invasion, while the expression of lncRNA MYU increased as cell proliferation increased. In addition, the mRNA expression of Vimentin and Ki‑67 was decreased in TE‑2 cells after lncRNA MYU was knocked down, while E‑cadherin mRNA expression was elevated. In conclusion, the present results indicated that lncRNA MYU may regulate the proliferation, migration and invasion of ESCC cells, and may serve as a prognostic biomarker for ESCC.

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