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Role of lincRNA‑Cox2 targeting miR‑150 in regulating the viability of chondrocytes in osteoarthritis

  • Authors:
    • Meng Jiang
    • Kai Xu
    • Huafeng Ren
    • Mingmin Wang
    • Ximin Hou
    • Jianping Cao
  • View Affiliations / Copyright

    Affiliations: Department of Orthopaedics, Qingdao No. 6 People's Hospital, Qingdao, Shandong 266033, P.R. China, Department of Functional Examination, Qingdao Haici Medical Group, Qingdao, Shandong 266033, P.R. China, Department of Anesthesiology, Qingdao No. 6 People's Hospital, Qingdao, Shandong 266033, P.R. China
    Copyright: © Jiang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 800
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    Published online on: May 25, 2021
       https://doi.org/10.3892/etm.2021.10232
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Abstract

Osteoarthritis (OA) is a joint disease characterised by progressive cartilage degradation and inflammation, but the detailed pathogenesis of OA remains unclear. The present study aimed to investigate the role of long intergenic non‑coding RNA (lincRNA)‑Cox2 in OA progression and the potential mechanism. An OA mouse model was used for in vivo experiments, and IL‑1β‑induced injury of mouse chondrocytes was conducted for in vitro experiments. Small interfering (si)‑Cox2 was transfected into chondrocytes to elucidate the effect of lincRNA‑Cox2 on OA. Quantitative reverse transcription PCR assays were conducted to detect the expression of lincRNA‑Cox2 and microRNA (miR)‑150. Cell proliferation and apoptosis were analysed based on an MTT assay and annexin V/propidium iodide staining, respectively. Western blotting was performed to evaluate the protein expression levels of Ki‑67, PCNA, Bax, cleaved (c)‑Caspase‑3, c‑Caspase‑9 and Wnt/β‑catenin pathway‑associated proteins in chondrocytes. High levels of lincRNA‑Cox2 were observed in cartilage tissues of the OA mouse model in vivo. In the in vitro experiments, the expression of lincRNA‑Cox2 was increased in IL‑1β‑treated chondrocytes. Knockdown of lincRNA‑Cox2 promoted the proliferation and inhibited the apoptosis of chondrocytes. Mechanistically, lincRNA‑Cox2 was found to directly target miR‑150, acting as a competing endogenous RNA, and the effect of si‑Cox2 on the proliferation and apoptosis of chondrocytes was reversed by miR‑150 inhibitors. Moreover, lincRNA‑Cox2 activated the Wnt/β‑catenin pathway to regulate chondrocyte proliferation and apoptosis. The present study demonstrated that silencing lincRNA‑Cox2 expression plays a protective role in OA by enhancing the proliferation and suppressing the apoptosis of chondrocytes, which is related to increased miR‑150 expression and activation of the Wnt/β‑catenin pathway.
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1 

Charlier E, Relic B, Deroyer C, Malaise O, Neuville S, Collée J, Malaise MG and De Seny D: Insights on molecular mechanisms of chondrocytes death in osteoarthritis. Int J Mol Sci. 17(2146)2016.PubMed/NCBI View Article : Google Scholar

2 

Eveque-Mourroux MR, Rocha B, Barre FPY, Heeren RMA and Cillero-Pastor B: Spatially resolved proteomics in osteoarthritis: State of the art and new perspectives. J Proteomics. 215(103637)2020.PubMed/NCBI View Article : Google Scholar

3 

Goldring M and Goldring S: Osteoarthritis. J Cell Physiol. 213:626–634. 2007.PubMed/NCBI View Article : Google Scholar

4 

Jiang S, Liu Y, Xu B, Zhang Y and Yang M: Noncoding RNAs: New regulatory code in chondrocyte apoptosis and autophagy. Wiley Interdiscip Rev RNA. 11(e1584)2020.PubMed/NCBI View Article : Google Scholar

5 

Zhu J, Yu W, Wang Y, Xia K, Huang Y, Xu A, Chen Q, Liu B, Tao H, Li F and Liang C: lncRNAs: Function and mechanism in cartilage development, degeneration, and regeneration. Stem Cell Res Ther. 10(344)2019.PubMed/NCBI View Article : Google Scholar

6 

Shui X, Xie Q, Chen S, Zhou C, Kong J and Wang Y: Identification and functional analysis of long non-coding RNAs in the synovial membrane of osteoarthritis patients. Cell Biochem Funct. 38:460–471. 2020.PubMed/NCBI View Article : Google Scholar

7 

Yuangang W, Xiaoxi L, Bin S and Yi Z: The therapeutic potential and role of miRNA, lncRNA, and circRNA in osteoarthritis. Curr Gene Ther. 19:255–263. 2019.PubMed/NCBI View Article : Google Scholar

8 

Chen YG, Satpathy AT and Chang HY: Gene regulation in the immune system by long noncoding RNAs. Nat Immunol. 18:962–972. 2017.PubMed/NCBI View Article : Google Scholar

9 

Luo H, Sun S, Li P, Bu D, Cao H and Zhao Y: Comprehensive characterization of 10,571 mouse large intergenic noncoding RNAs from whole transcriptome sequencing. PLoS One. 8(e70835)2013.PubMed/NCBI View Article : Google Scholar

10 

Terracciano D, Terreri S, de Nigris F, Costa V, Calin GA and Cimmino A: The role of a new class of long noncoding RNAs transcribed from ultraconserved regions in cancer. Biochim Biophys Acta Rev Cancer. 1868:449–455. 2017.PubMed/NCBI View Article : Google Scholar

11 

Dominguez-Andres J, Fanucchi S, Joosten LAB, Mhlanga MM and Netea MG: Advances in understanding molecular regulation of innate immune memory. Curr Opin Cell Biol. 63:68–75. 2020.PubMed/NCBI View Article : Google Scholar

12 

Carpenter S, Aiello D, Atianand MK, Ricci EP, Gandhi P, Hall LL, Byron M, Monks B, Henry-Bezy M, Lawrence JB, et al: A long noncoding RNA mediates both activation and repression of immune response genes. Science. 341:789–792. 2013.PubMed/NCBI View Article : Google Scholar

13 

Elling R, Robinson EK, Shapleigh B, Liapis SC, Covarrubias S, Katzman S, Groff AF, Jiang Z, Agarwal S, Motwani M, et al: Genetic models reveal cis and Trans immune-regulatory activities for lincRNA-Cox2. Cell Rep. 25:1511–1524.e6. 2018.PubMed/NCBI View Article : Google Scholar

14 

Tong Q, Gong AY, Zhang XT, Lin C, Ma S, Chen J, Hu G and Chen XM: lincRNA-Cox2 modulates TNF-α-induced transcription of Il12b gene in intestinal epithelial cells through regulation of Mi-2/NuRD-mediated epigenetic histone modifications. FASEB J. 30:1187–1197. 2016.PubMed/NCBI View Article : Google Scholar

15 

Brown AL, Al-Samadi A, Sperandio M, Soares AB, Teixeira LN, Martinez EF, Demasi APD, Araújo VC, Leivo I, Salo T and Passador-Santos F: miR-455-3p, miR-150 and miR-375 are aberrantly expressed in salivary gland adenoid cystic carcinoma and polymorphous adenocarcinoma. J Oral Pathol Med. 48:840–845. 2019.PubMed/NCBI View Article : Google Scholar

16 

Verma P, Pandey RK, Prajapati P and Prajapati VK: Circulating microRNAs: Potential and emerging biomarkers for diagnosis of human infectious diseases. Front Microbiol. 7(1274)2016.PubMed/NCBI View Article : Google Scholar

17 

Niimoto T, Nakasa T, Ishikawa M, Okuhara A, Izumi B, Deie M, Suzuki O, Adachi N and Ochi M: MicroRNA-146a expresses in interleukin-17 producing T cells in rheumatoid arthritis patients. BMC Musculoskelet Disord. 11(209)2010.PubMed/NCBI View Article : Google Scholar

18 

Sandell LJ and Aigner T: Articular cartilage and changes in arthritis. An introduction: Cell biology of osteoarthritis. Arthritis Res. 3:107–113. 2001.PubMed/NCBI View Article : Google Scholar

19 

Sun P, Wu Y, Li X and Jia Y: miR-142-5p protects against osteoarthritis through competing with lncRNA XIST. J Gene Med. 22(e3158)2020.PubMed/NCBI View Article : Google Scholar

20 

Lu X, Yu Y, Yin F, Yang C, Li B, Lin J and Yu H: Knockdown of PVT1 inhibits IL-1β-induced injury in chondrocytes by regulating miR-27b-3p/TRAF3 axis. Int Immunopharmacol. 79(106052)2020.PubMed/NCBI View Article : Google Scholar

21 

Lu C, Li Z, Hu S, Cai Y and Peng K: LncRNA PART-1 targets TGFBR2/Smad3 to regulate cell viability and apoptosis of chondrocytes via acting as miR-590-3p sponge in osteoarthritis. J Cell Mol Med. 23:8196–8205. 2019.PubMed/NCBI View Article : Google Scholar

22 

Gosset M, Berenbaum F, Thirion S and Jacques C: Primary culture and phenotyping of murine chondrocytes. Nat Protoc. 3:1253–1260. 2008.PubMed/NCBI View Article : Google Scholar

23 

Li Y, Wu Y, Jiang K, Han W, Zhang J, Xie L, Liu Y, Xiao J and Wang X: Mangiferin prevents TBHP-induced apoptosis and ECM degradation in mouse osteoarthritic chondrocytes via restoring autophagy and ameliorates murine osteoarthritis. Oxid Med Cell Longev. 2019(8783197)2019.PubMed/NCBI View Article : Google Scholar

24 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001.PubMed/NCBI View Article : Google Scholar

25 

Hawker GA: Osteoarthritis is a serious disease. Clin Exp Rheumatol. 120:3–6. 2019.PubMed/NCBI

26 

Xue Z, Zhang Z, Liu H, Li W, Guo X, Zhang Z, Liu Y, Jia L, Li Y, Ren Y, et al: lincRNA-Cox2 regulates NLRP3 inflammasome and autophagy mediated neuroinflammation. Cell Death Differ. 26:130–145. 2019.PubMed/NCBI View Article : Google Scholar

27 

Jurikova M, Danihel L, Polak S and Varga I: Ki67, PCNA, and MCM proteins: Markers of proliferation in the diagnosis of breast cancer. Acta Histochem. 118:544–552. 2016.PubMed/NCBI View Article : Google Scholar

28 

Yan X, Zhou R and Ma Z: Autophagy-cell survival and death. In: Autophagy: Biology and Diseases: Basic Science. Qin ZH (ed). Springer Singapore, Singapore, pp667-696, 2019.

29 

Aghaei M, KhanAhmad H, Aghaei S, Ali Nilforoushzadeh M, Mohaghegh MA and Hejazi SH: The role of Bax in the apoptosis of Leishmania-infected macrophages. Microb Pathog. 139(103892)2020.PubMed/NCBI View Article : Google Scholar

30 

Tay Y, Rinn J and Pandolfi PP: The multilayered complexity of ceRNA crosstalk and competition. Nature. 505:344–352. 2014.PubMed/NCBI View Article : Google Scholar

31 

Jeffries MA: Osteoarthritis year in review 2018: Genetics and epigenetics. Osteoarthritis Cartilage. 27:371–377. 2019.PubMed/NCBI View Article : Google Scholar

32 

Zhang Y, Wang F, Chen G, He R and Yang L: LncRNA MALAT1 promotes osteoarthritis by modulating miR-150-5p/AKT3 axis. Cell Biosci. 9(54)2019.PubMed/NCBI View Article : Google Scholar

33 

Takahashi T: Roles of nAChR and Wnt signaling in intestinal stem cell function and inflammation. Int Immunopharmacol. 81(106260)2020.PubMed/NCBI View Article : Google Scholar

34 

Huang J, Chen C, Liang C, Luo P, Xia G, Zhang L, Wang X, Wen Z, Cao X and Wu S: Dysregulation of the Wnt signaling pathway and synovial stem cell dysfunction in osteoarthritis development. Stem Cells Dev. 29:401–413. 2020.PubMed/NCBI View Article : Google Scholar

35 

Xiaoliang Y, Haiqing L, Hao H, Hai L, Linfu L, Jianqiong Y, Weimei S, Weiyou L and Longhuo W: The key role of canonical Wntβ-catenin signaling in cartilage chondrocytes. Curr Drug Targets. 17:475–484. 2016.PubMed/NCBI View Article : Google Scholar

36 

Vallée A and Lecarpentier Y: Crosstalk between peroxisome proliferator-activated receptor gamma and the canonical WNT/β-catenin pathway in chronic inflammation and oxidative stress during carcinogenesis. Front Immunol. 9(745)2018.PubMed/NCBI View Article : Google Scholar

37 

Mao T, He C, Wu H, Yang B and Li X: Silencing lncRNA HOTAIR declines synovial inflammation and synoviocyte proliferation and promotes synoviocyte apoptosis in osteoarthritis rats by inhibiting Wnt/β-catenin signaling pathway. Cell Cycle. 18:3189–3205. 2019.PubMed/NCBI View Article : Google Scholar

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Copy and paste a formatted citation
Spandidos Publications style
Jiang M, Xu K, Ren H, Wang M, Hou X and Cao J: Role of lincRNA‑Cox2 targeting miR‑150 in regulating the viability of chondrocytes in osteoarthritis. Exp Ther Med 22: 800, 2021.
APA
Jiang, M., Xu, K., Ren, H., Wang, M., Hou, X., & Cao, J. (2021). Role of lincRNA‑Cox2 targeting miR‑150 in regulating the viability of chondrocytes in osteoarthritis. Experimental and Therapeutic Medicine, 22, 800. https://doi.org/10.3892/etm.2021.10232
MLA
Jiang, M., Xu, K., Ren, H., Wang, M., Hou, X., Cao, J."Role of lincRNA‑Cox2 targeting miR‑150 in regulating the viability of chondrocytes in osteoarthritis". Experimental and Therapeutic Medicine 22.2 (2021): 800.
Chicago
Jiang, M., Xu, K., Ren, H., Wang, M., Hou, X., Cao, J."Role of lincRNA‑Cox2 targeting miR‑150 in regulating the viability of chondrocytes in osteoarthritis". Experimental and Therapeutic Medicine 22, no. 2 (2021): 800. https://doi.org/10.3892/etm.2021.10232
Copy and paste a formatted citation
x
Spandidos Publications style
Jiang M, Xu K, Ren H, Wang M, Hou X and Cao J: Role of lincRNA‑Cox2 targeting miR‑150 in regulating the viability of chondrocytes in osteoarthritis. Exp Ther Med 22: 800, 2021.
APA
Jiang, M., Xu, K., Ren, H., Wang, M., Hou, X., & Cao, J. (2021). Role of lincRNA‑Cox2 targeting miR‑150 in regulating the viability of chondrocytes in osteoarthritis. Experimental and Therapeutic Medicine, 22, 800. https://doi.org/10.3892/etm.2021.10232
MLA
Jiang, M., Xu, K., Ren, H., Wang, M., Hou, X., Cao, J."Role of lincRNA‑Cox2 targeting miR‑150 in regulating the viability of chondrocytes in osteoarthritis". Experimental and Therapeutic Medicine 22.2 (2021): 800.
Chicago
Jiang, M., Xu, K., Ren, H., Wang, M., Hou, X., Cao, J."Role of lincRNA‑Cox2 targeting miR‑150 in regulating the viability of chondrocytes in osteoarthritis". Experimental and Therapeutic Medicine 22, no. 2 (2021): 800. https://doi.org/10.3892/etm.2021.10232
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