Optimization of a GF‑AAS method for lead testing in blood and urine: A useful tool in acute abdominal pain management in emergency

  • Authors:
    • Mihail Silviu Tudosie
    • Genica Caragea
    • Dragos Marian Popescu
    • Oana Avram
    • Dragos Serban
    • Catalin Gabriel Smarandache
    • Corneliu Tudor
    • Cristinel Dumitru Badiu
    • Bogdan Socea
    • Alexandru Dan Sabau
    • Meda Comandasu
    • Radu Spataru
    • Daniel Ovidiu Costea
    • Ciprian Tanasescu
    • Ana Maria Dascalu
  • View Affiliations

  • Published online on: July 13, 2021     https://doi.org/10.3892/etm.2021.10417
  • Article Number: 985
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Abstract

Suspicion of lead poisoning is confirmed by its concentration in blood and protoporphyrin red blood cells. At low concentrations, lead influences the synthesis of the heme in the sense of lowering it. Acute and chronic lead intoxication is extremely polymorphic in regards to its clinical manifestations, with digestive, hematological, cardiovascular, renal hepatic and neurological features. The aim of the study was to evaluate the presence of lead in human whole blood and urine harvested before and during chelation treatment in the case of lead poisoning. An atomic absorption spectroscopic method for the analysis of lead was developed using graphite furnace atomic absorption spectrophotometer (GF‑AAS), Varian Spectra AA‑880 with a hollow cathode lead lamp and a deuterium lamp for background correction, coupled to a GTA‑100 atomizer and a programmable sample dispenser. Standard calibration solutions were used for the range 10‑100 µg/l. The linearity range was 10.0 to 100.0 µg/l with the correlation coefficient of 0.999. We established that the method can be applied for the determination of lead in whole blood and urine, and the results obtained are useful for monitoring chelation therapy in cases of acute lead poisoning, a neglected cause of abdominal colic pain in an emergency situation.

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September-2021
Volume 22 Issue 3

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Tudosie MS, Caragea G, Popescu DM, Avram O, Serban D, Smarandache CG, Tudor C, Badiu CD, Socea B, Dan Sabau A, Dan Sabau A, et al: Optimization of a GF‑AAS method for lead testing in blood and urine: A useful tool in acute abdominal pain management in emergency. Exp Ther Med 22: 985, 2021
APA
Tudosie, M.S., Caragea, G., Popescu, D.M., Avram, O., Serban, D., Smarandache, C.G. ... Dascalu, A.M. (2021). Optimization of a GF‑AAS method for lead testing in blood and urine: A useful tool in acute abdominal pain management in emergency. Experimental and Therapeutic Medicine, 22, 985. https://doi.org/10.3892/etm.2021.10417
MLA
Tudosie, M. S., Caragea, G., Popescu, D. M., Avram, O., Serban, D., Smarandache, C. G., Tudor, C., Badiu, C. D., Socea, B., Dan Sabau, A., Comandasu, M., Spataru, R., Costea, D. O., Tanasescu, C., Dascalu, A. M."Optimization of a GF‑AAS method for lead testing in blood and urine: A useful tool in acute abdominal pain management in emergency". Experimental and Therapeutic Medicine 22.3 (2021): 985.
Chicago
Tudosie, M. S., Caragea, G., Popescu, D. M., Avram, O., Serban, D., Smarandache, C. G., Tudor, C., Badiu, C. D., Socea, B., Dan Sabau, A., Comandasu, M., Spataru, R., Costea, D. O., Tanasescu, C., Dascalu, A. M."Optimization of a GF‑AAS method for lead testing in blood and urine: A useful tool in acute abdominal pain management in emergency". Experimental and Therapeutic Medicine 22, no. 3 (2021): 985. https://doi.org/10.3892/etm.2021.10417