Open Access

Anti‑inflammatory effects of miR‑150 are associated with the downregulation of STAT1 in macrophages following lipopolysaccharide treatment

  • Authors:
    • Song Chen
    • Haijun Zhu
    • Jie Sun
    • Lili Zhu
    • Long Qin
    • Jian Wan
  • View Affiliations

  • Published online on: July 23, 2021     https://doi.org/10.3892/etm.2021.10483
  • Article Number: 1049
  • Copyright: © Chen et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Sepsis is a condition that is associated with high rates of mortality. It is characterized by serious systemic inflammatory responses induced by pathogenic invasion. Although microRNA‑150 (miR‑150) has been previously reported to be involved in the modulation of sepsis, the underlying molecular mechanism in sepsis remains poorly understood. In the present study, the human monocytic cell line THP‑1 was treated with LPS to mimic sepsis in vitro, following which miR‑150 and STAT1 expression were measured using reverse transcription‑quantitative PCR or western blotting. Secretion of inflammatory cytokines interleukin (IL)‑1β, IL‑6 and tumor necrosis factor‑α (TNF‑α) into the medium were measured by ELISA. The potential relationship between STAT1 and miR‑150 was determined using dual‑luciferase reporter and RNA immunoprecipitation assays. miR‑150 expression was found to be was downregulated by LPS treatment in THP‑1 cells in both dose‑ and time‑dependent manners. LPS treatment also induced IL‑1β, IL‑6 and TNF‑α secretion in a manner that could be inhibited by miR‑150 overexpression and enhanced by transfection with the miR‑150 inhibitor. miR‑150 was revealed to directly target STAT1 by negatively regulating its expression. In addition, STAT1 expression was demonstrated to be upregulated by LPS treatment. STAT1 overexpression reversed the inhibitory effects of miR‑150 overexpression on IL‑1β, IL‑6 and TNF‑α secretion whilst STAT1 knockdown attenuated IL‑1β, IL‑6 and TNF‑α secretion induced by miR‑150 inhibitor transfection. In conclusion, the present study suggested that miR‑150 regulates the inflammatory response in macrophages following LPS challenge by regulating the expression of STAT1.
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October-2021
Volume 22 Issue 4

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Chen S, Zhu H, Sun J, Zhu L, Qin L and Wan J: Anti‑inflammatory effects of miR‑150 are associated with the downregulation of STAT1 in macrophages following lipopolysaccharide treatment. Exp Ther Med 22: 1049, 2021
APA
Chen, S., Zhu, H., Sun, J., Zhu, L., Qin, L., & Wan, J. (2021). Anti‑inflammatory effects of miR‑150 are associated with the downregulation of STAT1 in macrophages following lipopolysaccharide treatment. Experimental and Therapeutic Medicine, 22, 1049. https://doi.org/10.3892/etm.2021.10483
MLA
Chen, S., Zhu, H., Sun, J., Zhu, L., Qin, L., Wan, J."Anti‑inflammatory effects of miR‑150 are associated with the downregulation of STAT1 in macrophages following lipopolysaccharide treatment". Experimental and Therapeutic Medicine 22.4 (2021): 1049.
Chicago
Chen, S., Zhu, H., Sun, J., Zhu, L., Qin, L., Wan, J."Anti‑inflammatory effects of miR‑150 are associated with the downregulation of STAT1 in macrophages following lipopolysaccharide treatment". Experimental and Therapeutic Medicine 22, no. 4 (2021): 1049. https://doi.org/10.3892/etm.2021.10483