Fas regulates the apoptosis and migration of trophoblast cells by targeting NF‑κB

Lan,Ruihong; Yang,Yang; Song,Jie; Wang,Ling; Gong,Humin

doi:10.3892/etm.2021.10489

Fas regulates the apoptosis and migration of trophoblast cells by targeting NF‑κB

Authors:
- Ruihong Lan
- Yang Yang
- Jie Song
- Ling Wang
- Humin Gong
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Affiliations: Department of Obstetrics, Hainan General Hospital/Affiliated Hainan Hospital of Hainan Medical College, Haikou, Hainan 570311, P.R. China
Published online on: July 23, 2021 https://doi.org/10.3892/etm.2021.10489
Article Number: 1055
Copyright: © Lan et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Placental trophoblast apoptosis is a major pathological feature of preeclampsia. Fas has been reported to be highly expressed in the placentas of patients with preeclampsia. However, the role and underlying mechanisms of Fas in the pathogenesis of preeclampsia have not been elucidated. In the present study, the expression of Fas in JAR human choriocarcinoma cells was overexpressed and knocked down to determine the function and possible mechanism of Fas in trophoblast cells in the progression of preeclampsia. The results of flow cytometry, Cell Counting Kit‑8 and Transwell assays indicated that the overexpression of Fas promoted apoptosis, suppressed viability and impaired the migration of the human trophoblast cells. In addition, western blotting revealed that the overexpression of Fas increased the expression of nuclear factor kB (NF‑kB), Bax, tumor necrosis factor α (TNF‑α) and interleukin‑2 (IL‑2), and decreased the expression of Bcl‑2 at the protein level in trophoblast cells. By contrast, the knockdown of Fas decreased the apoptosis of trophoblast cells and increased their viability and migration. In addition, the knockdown of Fas suppressed the expression of NF‑κB, Bax, TNF‑α and IL‑2, and increased the expression of Bcl‑2. Notably, the overexpression of NF‑κB p65 attenuated the Fas knockdown‑induced inhibition of apoptosis and acceleration of migration of the trophoblast cells. The overexpression of NF‑κB in trophoblast cells also reversed the reduction in Bax expression and increase in Bcl‑2 expression induced by Fas knockdown in trophoblast cells. These results indicate that Fas regulates the apoptosis and migration of trophoblast cells by targeting NF‑κB, which suggests that the silencing of Fas is a promising therapeutic strategy for preeclampsia.

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