Involvement of COL5A2 and TGF‑β1 in pathological scarring

Ilieș,Roxana Flavia; Aioanei,Casian Simon; Cătană,Andreea; Halmagyi,Salomea-Ruth; Lukacs,Istvan; Tokes,Reka-Eniko; Rotar,Ioana Cristina; Pop,Ioan Victor

doi:10.3892/etm.2021.10501

October-2021 Volume 22 Issue 4

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October-2021 Volume 22 Issue 4

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Article

Involvement of COL5A2 and TGF‑β1 in pathological scarring

Authors:
- Roxana Flavia Ilieș
- Casian Simon Aioanei
- Andreea Cătană
- Salomea-Ruth Halmagyi
- Istvan Lukacs
- Reka-Eniko Tokes
- Ioana Cristina Rotar
- Ioan Victor Pop
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Affiliations: Department of Medical Genetics, ‘Iuliu Hațieganu’ University of Medicine and Pharmacy, 400349 Cluj‑Napoca, Romania, First Department of Obstetrics and Gynecology, ‘Iuliu Hațieganu’ University of Medicine and Pharmacy, 400006 Cluj‑Napoca, Romania
Article Number: 1067
|
Published online on: July 27, 2021

https://doi.org/10.3892/etm.2021.10501
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Abstract

Dysregulation in the cutaneous wound‑healing process is a consequence of alterations in the efficiency and activity of the various components involved in the healing process. This dysregulation may result in various clinical appearances of a lesion, such as skin ulcers, keloids, hypertrophic and atrophic scars. The collagen type V alpha 2 (COL5A2) gene provides a template for a component of type V collagen, found primarily within the skin basement membrane. Transforming growth factor (TGF)‑β is involved in inflammation, angiogenesis, proliferation of fibroblasts, collagen synthesis and extracellular matrix remodeling. Hypertrophic scar fibroblasts possess a disrupted expression pattern of the TGF‑β signaling compared to normal healing, while an increased TGF‑β signaling reduces the epidermal proliferation rate, triggering atrophic scarring. In the present study, 71 female patients who had undergone planned Caesarean section, without postoperative complications, were examined. These patients were clinically and molecularly evaluated after developing scars in order to determine the role of TGF‑β1 (rs201700967 and rs200230083) and COL5A2 (rs369072636) in pathological scarring. Clinical scar evaluation was carried out using SCAR and POSAS scales and genotyping was performed by RT‑PCR. No statistical differences were found between the subgroups regarding the genotype and the pathological scarring, since all the patients included were wild‑type allele carriers. Further investigations and a more representative study group may highlight the involvement of COL5A2 and TGF‑β1 single nucleotide variants in pathological scarring.

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1	Turabelidze A, Guo S and DiPietro LA: Importance of housekeeping gene selection for accurate reverse transcription-quantitative polymerase chain reaction in a wound healing model. Wound Repair Regen. 18:460–466. 2010.PubMed/NCBI View Article : Google Scholar
2	Xue M and Jackson CJ: Extracellular matrix reorganization during wound healing and its impact on abnormal scarring. Adv Wound Care (New Rochelle). 4:119–136. 2015.PubMed/NCBI View Article : Google Scholar
3	Fathke C, Wilson L, Shah K, Kim B, Hocking A, Moon R and Isik F: Wnt signaling induces epithelial differentiation during cutaneous wound healing. BMC Cell Biol. 7(4)2006.PubMed/NCBI View Article : Google Scholar
4	Abreu-Velez AM and Howard MS: Collagen IV in normal skin and in pathological processes. N Am J Med Sci. 4:1–8. 2012.PubMed/NCBI View Article : Google Scholar
5	Malfait F, Wenstrup RJ and De Paepe A: Clinical and genetic aspects of Ehlers-Danlos syndrome, classic type. Genet Med. 12:597–605. 2010.PubMed/NCBI View Article : Google Scholar
6	Park AC, Phan N, Massoudi D, Liu Z, Kernien JF, Adams SM, Davidson JM, Birk DE, Liu B and Greenspan DS: Deficits in Col5a2 expression result in novel skin and adipose abnormalities and predisposition to aortic aneurysms and dissections. Am J Pathol. 187:2300–2311. 2017.PubMed/NCBI View Article : Google Scholar
7	Mokos ZB, Jović A, Grgurević L, Dumić-Čule I, Kostović K, Čeović R and Marinović B: Current therapeutic approach to hypertrophic scars. Front Med (Lausanne). 4(83)2017.PubMed/NCBI View Article : Google Scholar
8	Ilie MA, Caruntu C, Georgescu SR, Matei C, Negrei C, Ion RM, Constantin C, Neagu M and Boda D: Capsaicin: Physicochemical properties, cutaneous reactions and potential applications in painful and inflammatory conditions. Exp Ther Med. 18:916–925. 2019.PubMed/NCBI View Article : Google Scholar
9	Penn JW, Grobbelaar AO and Rolfe KJ: The role of the TGF-β family in wound healing, burns and scarring: A review. Int J Burns Trauma. 2:18–28. 2012.PubMed/NCBI
10	Moon J, Yoon JY, Yang JH, Kwon HH, Min S and Suh DH: Atrophic acne scar: A process from altered metabolism of elastic fibres and collagen fibres based on transforming growth factor-β1 signalling. Br J Dermatol. 181:1226–1237. 2019.PubMed/NCBI View Article : Google Scholar
11	Kantor J: The SCAR (Scar Cosmesis Assessment and Rating) scale: Development and validation of a new outcome measure for postoperative scar assessment. Br J Dermatol. 175:1394–1396. 2016.PubMed/NCBI View Article : Google Scholar
12	Rabello FB, Souza CD and Farina Júnior JA: Update on hypertrophic scar treatment. Clinics (Sao Paulo). 69:565–573. 2014.PubMed/NCBI View Article : Google Scholar
13	Rorison P, Thomlinson A, Hassan Z, Roberts SA, Ferguson MWJ and Shah M: Longitudinal changes in plasma Transforming growth factor beta-1 and post-burn scarring in children. Burns. 36:89–96. 2010.PubMed/NCBI View Article : Google Scholar
14	Wang J, Jiao H, Stewart TL, Shankowsky HA, Scott PG and Tredget EE: Increased TGF-β-producing CD4⁺ T lymphocytes in postburn patients and their potential interaction with dermal fibroblasts in hypertrophic scarring. Wound Repair Regen. 15:530–539. 2007.PubMed/NCBI View Article : Google Scholar
15	Shah M, Foreman DM and Ferguson MWJ: Neutralisation of TGF-β 1 and TGF-β 2 or exogenous addition of TGF-β 3 to cutaneous rat wounds reduces scarring. J Cell Sci. 108:985–1002. 1995.PubMed/NCBI
16	Mao JH, Saunier EF, de Koning JP, McKinnon MM, Higgins MN, Nicklas K, Yang HT, Balmain A and Akhurst RJ: Genetic variants of Tgfb1 act as context-dependent modifiers of mouse skin tumor susceptibility. Proc Natl Acad Sci USA. 103:8125–8130. 2006.PubMed/NCBI View Article : Google Scholar

Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Involvement of COL5A2 and TGF‑β1 in pathological scarring

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