Protectin D1 protects against lipopolysaccharide‑induced acute lung injury through inhibition of neutrophil infiltration and the formation of neutrophil extracellular traps in lung tissue

  • Authors:
    • Zhiyang Wu
    • Luyao Zhang
    • Xiangyang Zhao
    • Zhi Li
    • Haining Lu
    • Chanyuan Bu
    • Rui Wang
    • Xiaofei Wang
    • Tiantian Cai
    • Dawei Wu
  • View Affiliations

  • Published online on: July 28, 2021     https://doi.org/10.3892/etm.2021.10508
  • Article Number: 1074
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Abstract

Protectin D1 (PD1), a DHA‑derived lipid mediator, has recently been shown to possess anti‑inflammatory and pro‑resolving properties. To date, little is known about the effect of PD1 on lipopolysaccharide (LPS)‑induced acute lung injury (ALI) in mice. The aim of the present study was to investigate the therapeutic effects of PD1 on LPS‑induced ALI and its potential mechanisms of action. ALI was induced via an intraperitoneal injection of LPS, where PD1 (2 ng/mouse) was administered intravenously 30 min after LPS challenge. Mice were sacrificed 24 h after modeling. Lung histopathological changes were assessed using hematoxylin and eosin staining and myeloperoxidase (MPO) activity was tested using immunohistochemistry. Tumor necrosis‑α and interleukin‑6 levels in the bronchoalveolar lavage fluid (BALF) and serum were measured using ELISA. To detect neutrophil extracellular traps produced by infiltrated neutrophils in the lung tissue, immunofluorescence staining was performed using anti‑MPO and anti‑histone H3 antibodies. The results indicated that PD1 significantly attenuated histological damage and neutrophil infiltration in lung tissue, reduced the lung wet/dry weight ratio, protein concentration and proinflammatory cytokine levels in BALF and decreased proinflammatory cytokine levels in serum. Moreover, neutrophil citrullinated histone H3 (CitH3) expression was also reduced after PD1 administration. In conclusion, PD1 attenuated LPS‑induced ALI in mice via inhibition of neutrophil extracellular trap formation in lung tissue. Therefore, PD1 administration may serve to be a new strategy for treating ALI.
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October-2021
Volume 22 Issue 4

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Wu Z, Zhang L, Zhao X, Li Z, Lu H, Bu C, Wang R, Wang X, Cai T, Wu D, Wu D, et al: Protectin D1 protects against lipopolysaccharide‑induced acute lung injury through inhibition of neutrophil infiltration and the formation of neutrophil extracellular traps in lung tissue. Exp Ther Med 22: 1074, 2021
APA
Wu, Z., Zhang, L., Zhao, X., Li, Z., Lu, H., Bu, C. ... Wu, D. (2021). Protectin D1 protects against lipopolysaccharide‑induced acute lung injury through inhibition of neutrophil infiltration and the formation of neutrophil extracellular traps in lung tissue. Experimental and Therapeutic Medicine, 22, 1074. https://doi.org/10.3892/etm.2021.10508
MLA
Wu, Z., Zhang, L., Zhao, X., Li, Z., Lu, H., Bu, C., Wang, R., Wang, X., Cai, T., Wu, D."Protectin D1 protects against lipopolysaccharide‑induced acute lung injury through inhibition of neutrophil infiltration and the formation of neutrophil extracellular traps in lung tissue". Experimental and Therapeutic Medicine 22.4 (2021): 1074.
Chicago
Wu, Z., Zhang, L., Zhao, X., Li, Z., Lu, H., Bu, C., Wang, R., Wang, X., Cai, T., Wu, D."Protectin D1 protects against lipopolysaccharide‑induced acute lung injury through inhibition of neutrophil infiltration and the formation of neutrophil extracellular traps in lung tissue". Experimental and Therapeutic Medicine 22, no. 4 (2021): 1074. https://doi.org/10.3892/etm.2021.10508