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Emodin inhibits the proliferation of papillary thyroid carcinoma by activating AMPK

  • Authors:
    • Weilong Li
    • Dong Wang
    • Meijing Li
    • Baoyuan Li
  • View Affiliations / Copyright

    Affiliations: Department of Nuclear Medicine, The Affiliated Yantai Yuhuangding Hospital of Qingdao University, Yantai, Shandong 264000, P.R. China, Department of Thyroid, The Affiliated Yantai Yuhuangding Hospital of Qingdao University, Yantai, Shandong 264000, P.R. China, Department of Hepatobiliary, The Affiliated Yantai Yuhuangding Hospital of Qingdao University, Yantai, Shandong 264000, P.R. China
    Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 1075
    |
    Published online on: July 28, 2021
       https://doi.org/10.3892/etm.2021.10509
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Abstract

Emodin has been demonstrated to serve antitumor roles in a variety of tumor types, but the effect of emodin on papillary thyroid carcinoma and its molecular mechanisms remain unclear. In the current study, the role of emodin on papillary thyroid carcinoma was analyzed in vitro and in vivo. TPC‑1 cells were treated with emodin (0, 10, 25 or 50 µM), and cell viability and apoptosis were detected using Cell Counting Kit‑8 and flow cytometry, respectively. The expression levels of AMPK‑associated proteins were examined using western blot analysis. To study the effect of emodin on the AMPK pathway, AMPK activator, AICAR and an AMPK inhibitor, Dorsomorphin, were used in TPC‑1 cells. In vivo, mice were used to confirm the mechanism of emodin on papillary thyroid carcinoma. The results of the current study indicated that emodin treatment induced cell apoptosis and cell cycle arrest in TPC‑1 cells. Furthermore, the inhibitory effect increased in a dose dependent manner. Following emodin treatment, the cell viability of TPC‑1 cells was significantly decreased, and apoptosis rate increased (P<0.05). Furthermore, the expression levels of AMPK were increased in the emodin group compared with the control group (P<0.05). Similar effects were observed following AMPK activator treatment in TPC‑1 cells. Following AMPK activator treatment, cell proliferation and the cell cycle were inhibited. Also, the AMPK inhibitor was demonstrated to mediate the therapeutic effect of emodin. In addition, the results of the present study demonstrated that emodin inhibited the MEK/ERK pathway. Additionally, the in vivo results of the current study were consistent with those in vitro. In conclusion, the current study demonstrated that the administration of Emodin inhibited the proliferation of papillary thyroid cancer cells via activating AMPK pathway activity.
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Copy and paste a formatted citation
Spandidos Publications style
Li W, Wang D, Li M and Li B: Emodin inhibits the proliferation of papillary thyroid carcinoma by activating AMPK. Exp Ther Med 22: 1075, 2021.
APA
Li, W., Wang, D., Li, M., & Li, B. (2021). Emodin inhibits the proliferation of papillary thyroid carcinoma by activating AMPK. Experimental and Therapeutic Medicine, 22, 1075. https://doi.org/10.3892/etm.2021.10509
MLA
Li, W., Wang, D., Li, M., Li, B."Emodin inhibits the proliferation of papillary thyroid carcinoma by activating AMPK". Experimental and Therapeutic Medicine 22.4 (2021): 1075.
Chicago
Li, W., Wang, D., Li, M., Li, B."Emodin inhibits the proliferation of papillary thyroid carcinoma by activating AMPK". Experimental and Therapeutic Medicine 22, no. 4 (2021): 1075. https://doi.org/10.3892/etm.2021.10509
Copy and paste a formatted citation
x
Spandidos Publications style
Li W, Wang D, Li M and Li B: Emodin inhibits the proliferation of papillary thyroid carcinoma by activating AMPK. Exp Ther Med 22: 1075, 2021.
APA
Li, W., Wang, D., Li, M., & Li, B. (2021). Emodin inhibits the proliferation of papillary thyroid carcinoma by activating AMPK. Experimental and Therapeutic Medicine, 22, 1075. https://doi.org/10.3892/etm.2021.10509
MLA
Li, W., Wang, D., Li, M., Li, B."Emodin inhibits the proliferation of papillary thyroid carcinoma by activating AMPK". Experimental and Therapeutic Medicine 22.4 (2021): 1075.
Chicago
Li, W., Wang, D., Li, M., Li, B."Emodin inhibits the proliferation of papillary thyroid carcinoma by activating AMPK". Experimental and Therapeutic Medicine 22, no. 4 (2021): 1075. https://doi.org/10.3892/etm.2021.10509
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