Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Experimental and Therapeutic Medicine
Join Editorial Board Propose a Special Issue
Print ISSN: 1792-0981 Online ISSN: 1792-1015
Journal Cover
October-2021 Volume 22 Issue 4

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
October-2021 Volume 22 Issue 4

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML

  • Supplementary Files
    • Supplementary_Data1.xls
    • Supplementary_Data2.xls
    • Supplementary_Data3.xls
Article Open Access

Osteogenic ability using porous hydroxyapatite scaffold‑based delivery of human placenta‑derived mesenchymal stem cells

  • Authors:
    • Xiaohua Ren
    • Qingwei Wang
    • Chunhui Liu
    • Qian Zhao
    • Jiajun Zheng
    • Kun Tian
    • Huijuan Xu
    • Yandong Mu
  • View Affiliations / Copyright

    Affiliations: Stomatology Department, Sichuan Provincial People's Hospital, University of Electronic Science and Technology of China, Chengdu, Sichuan 610072, P.R. China, Institute of Chengdu Biology and Sichuan Translational Medicine Hospital, Chinese Academy of Sciences, Chengdu, Sichuan 610041, P.R. China, Shuangliu Hospital of Traditional Chinese Medicine, Chengdu, Sichuan 610000, P.R. China, Southwest Medical University, Luzhou, Sichuan 646000, P.R. China
    Copyright: © Ren et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 1091
    |
    Published online on: August 2, 2021
       https://doi.org/10.3892/etm.2021.10525
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Previous preliminary studies have suggested that hydroxyapatite with a grooved structure (HAG) scaffold has good osteogenic potential. This type of scaffold may aid osteogenesis during the repair of large maxillofacial bony defects. The ectopic osteogenic effect and underlying mechanism were further studied using porous HAG scaffold‑based delivery of human placenta‑derived mesenchymal stem cells (hPMSCs). A total of 18 dogs were randomly allocated into a HAG scaffold group and a HAG scaffold‑based hPMSC (HAG/hPMSC) group, and three scaffolds were implanted into the dorsal muscle of each dog. Samples were taken for subsequent analysis and tested 4, 8 and 12 weeks following heterotopic implantation. H&E staining was used to study the osteogenic effect in dog dorsal muscles, and RNA sequencing (RNA‑seq) was used for exploring the underlying osteogenic mechanism. The osteogenic ability and effector of the HAG/hPMSC group were significantly greater than those of the HAG scaffold group at 4 weeks after implantation. After 12 weeks, a mature bone plate structure was seen in the HAG/hPMSC group. RNA‑seq demonstrated that various osteogenesis‑related pathways participated at different stages of metabolism, and that the expression of collagen‑1 and runt‑related transcription factor 2 increased with implantation time. The present study preliminarily focused on the ectopic osteogenic effect of the porous HAG scaffold‑based delivery of hPMSCs in vivo, which may be helpful for the improved application of HAG scaffolds in the future.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

View References

1 

Cheng X, Lei D, Mao T, Yang S, Chen F and Wu W: Repair of critical bone defects with injectable platelet rich plasma/bone marrow-derived stromal cells composite: Experimental study in rabbits. Ulus Travma Acil Cerrahi Derg. 14:87–95. 2008.PubMed/NCBI

2 

Marx RE, Miller RI, Ehler WJ, Hubbard G and Malinin TI: A comparison of particulate allogeneic and particulate autogenous bone grafts into maxillary alveolar clefts in dogs. J Oral Maxillofac Surg. 42:3–9. 1984.PubMed/NCBI View Article : Google Scholar

3 

Janicki P and Schmidmaier G: What should be the characteristics of the ideal bone graft substitute? Combining scaffolds with growth factors and/or stem cells. Injury. 42 (Suppl 2):S77–S81. 2011.PubMed/NCBI View Article : Google Scholar

4 

Suresh SS, Raniga S, Shanmugam V, George M and Zaki H: Carpal tunnel syndrome due to hydroxyapatite crystal deposition disease. J Hand Microsurg. 5:96–99. 2013.PubMed/NCBI View Article : Google Scholar

5 

Xiaohua R, Qiang T, Kun T, Huang GT, Li JY, Xu T, Lv X, Wu J, Chen Z, Weng J, et al: Enhancement of osteogenesis using a novel porous hydroxyapatite scaffold in vivo and vitro. Ceram Int. 44:21656–21665. 2018.

6 

Wang Z, Gerstein M and Snyder M: RNA-Seq: A revolutionary tool for transcriptomics. Nat Rev Genet. 10:57–63. 2009.PubMed/NCBI View Article : Google Scholar

7 

Carnes MU, Allingham RR, Ashley-Koch A and Hauser MA: Transcriptome analysis of adult and fetal trabecular meshwork, cornea, and ciliary body tissues by RNA sequencing. Exp Eye Res. 167:91–99. 2018.PubMed/NCBI View Article : Google Scholar

8 

Cloonan N, Forrest AR, Kolle G, Gardiner BB, Faulkner GJ, Brown MK, Taylor DF, Steptoe AL, Wani S, Bethel G, et al: Stem cell transcriptome profiling via massive-scale mRNA sequencing. Nat Methods. 5:613–619. 2008.PubMed/NCBI View Article : Google Scholar

9 

Mortazavi A, Williams BA, McCue K, Schaeffer L and Wold B: Mapping and quantifying mammalian transcriptomes by RNA-Seq. Nat Methods. 5:621–628. 2008.PubMed/NCBI View Article : Google Scholar

10 

Trapnell C, Williams BA, Pertea G, Mortazavi A, Kwan G, van Baren MJ, Salzberg SL, Wold BJ and Pachter L: Transcript assembly and quantification by RNA-Seq reveals unannotated transcripts and isoform switching during cell differentiation. Nat Biotechnol. 28:511–515. 2010.PubMed/NCBI View Article : Google Scholar

11 

Guttman M, Garber M, Levin JZ, Donaghey J, Robinson J, Adiconis X, Fan L, Koziol MJ, Gnirke A, Nusbaum C, et al: Ab initio reconstruction of cell type-specific transcriptomes in mouse reveals the conserved multi-exonic structure of lincRNAs. Nat Biotechnol. 28:503–510. 2010.PubMed/NCBI View Article : Google Scholar

12 

Satija NK, Sharma D, Afrin F, Tripathi RP and Gangenahalli G: High throughput transcriptome profiling of lithium stimulated human mesenchymal stem cells reveals priming towards osteoblastic lineage. PLoS One. 8(e55769)2013.PubMed/NCBI View Article : Google Scholar

13 

Dominici M, Le Blanc K, Mueller I, Slaper-Cortenbach I, Marini F, Krause D, Deans R, Keating A, Prockop DJ and Horwitz E: Minimal criteria for defining multipotent mesenchymal stromal cells. The international society for cellular therapy position statement. Cytotherapy. 8:315–317. 2006.PubMed/NCBI View Article : Google Scholar

14 

Squillaro T, Peluso G and Galderisi U: Clinical trials with mesenchymal stem cells: An Update. Cell Transplant. 25:829–848. 2016.PubMed/NCBI View Article : Google Scholar

15 

Alessio N, Squillaro T, Özcan S, Di Bernardo G, Venditti M, Melone M, Peluso G and Galderisi U: Stress and stem cells: adult Muse cells tolerate extensive genotoxic stimuli better than mesenchymal stromal cells. Oncotarget. 9:19328–19341. 2018.PubMed/NCBI View Article : Google Scholar

16 

Fathi E and Farahzadi R: Zinc sulphate mediates the stimulation of cell proliferation of rat adipose tissue-derived mesenchymal stem cells under high intensity of EMF exposure. Biol Trace Elem Res. 184:529–535. 2017.PubMed/NCBI View Article : Google Scholar

17 

Fathi E, Farahzadi R and Sheikhzadeh N: Immunophenotypic characterization, multi-lineage differentiation and aging of zebrafish heart and liver tissue-derived mesenchymal stem cells as a novel approach in stem cell-based therapy. Tissue Cell. 57:15–21. 2019.PubMed/NCBI View Article : Google Scholar

18 

Lim J, Lee J, Yun HS, Park EK and Shin HI: Comparison of bone regeneration rate in flat and long bone defects: Calvarial and tibial bone. Tissue Eng Regene Med. 10:336–340. 2013.

19 

Lin YH, Chen CY, Chou LY, Chen CH, Kang L and Wang CZ: Enhancement of bone marrow-derived mesenchymal stem cell osteogenesis and new bone formation in rats by obtusilactone A. Int J Mol Sci. 18(2422)2017.PubMed/NCBI View Article : Google Scholar

20 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2002.PubMed/NCBI View Article : Google Scholar

21 

Wang YG, Han XG, Yang Y, Qiao H, Dai KR, Fan QM and Tang TT: Functional differences between AMPK alpha1 and alpha2 subunits in osteogenesis, osteoblast-associated induction of osteoclastogenesis, and adipogenesis. Sci Rep. 6(32771)2016.PubMed/NCBI View Article : Google Scholar

22 

Shu C, Zhao M, Anderson JP, Garg G, Singh KB, Zheng W, Wang C, Yang M and Zhou E: Transcriptome analysis reveals molecular mechanisms of sclerotial development in the rice sheath blight pathogen Rhizoctonia solani AG1-IA. Funct Integr Genomics. 19:743–758. 2015.PubMed/NCBI View Article : Google Scholar

23 

Liu Z, Yin X, Ye Q, He W and Zou S: Periodontal regeneration with stem cells-seeded collagen-hydroxyapatite scaffold. J Biomater Appl. 31(121)2016.PubMed/NCBI View Article : Google Scholar

24 

Wang G, Yang H, Li M, Lu S, Chen X and Cai X: The use of silk fibroin/hydroxyapatite composite co-cultured with rabbit bone-marrow stromal cells in the healing of a segmental bone defect. Bone Joint Surg Br. 92:320–325. 2010.PubMed/NCBI View Article : Google Scholar

25 

Diao Y, Ma Q, Cui F and Zhong Y: Human umbilical cord mesenchymal stem cells: Osteogenesis in vivo as seed cells for bone tissue engineering. J Biomed Mater Res Part A. 91A:123–131. 2010.PubMed/NCBI View Article : Google Scholar

26 

Liu Z, Yin X, Ye Q, He W, Ge M, Zhou X, Hu J and Zou S: Periodontal regeneration with stem cells-seeded collagen-hydroxyapatite scaffold. J Biomater Appl. 31:121–131. 2016.PubMed/NCBI View Article : Google Scholar

27 

Zhou L, Chen J, Li Z, Li X, Hu X, Huang Y, Zhao X, Liang C, Wang Y, Sun L, et al: Integrated profiling of microRNAs and mRNAs: microRNAs located on Xq27.3 associate with clear cell renal cell carcinoma. PLoS One. 5(e15224)2010.PubMed/NCBI View Article : Google Scholar

28 

Hung SC, Pochampally RR, Chen SC, Hsu SC and Prockop DJ: Angiogenic effects of human multipotent stromal cell conditioned medium activate the PI3K-Akt pathway in hypoxic endothelial cells to inhibit apoptosis, increase survival, and stimulate angiogenesis. Stem Cells. 25:2363–2370. 2007.PubMed/NCBI View Article : Google Scholar

29 

Wang YG, Qu XH, Yang Y, Han XG, Wang L, Qiao H, Fan QM, Tang TT and Dai KR: AMPK promotes osteogenesis and inhibits adipogenesis through AMPK-Gfi1-OPN axis. Cell Signal. 28:1270–1282. 2016.PubMed/NCBI View Article : Google Scholar

30 

Gupta V, Shah MA, Shah SK and Shah JM: Osteoanabolics. Indian J Endocrinol Metab. 6:349–357. 2012.PubMed/NCBI View Article : Google Scholar

31 

Zheng J, Mao X, Ling J, Chen C and Zhang W: Role of magnesium transporter subtype 1 (MagT1) in the osteogenic differentiation of rat bone marrow stem cells. Biol Trace Elem Res. 171:131–137. 2016.PubMed/NCBI View Article : Google Scholar

32 

Cui CB, Cooper LF, Yang X, Karsenty G and Aukhil I: Transcriptional coactivation of bone-specific transcription factor Cbfa1 by TAZ. Mol Cell Biol. 23:1004–1013. 2003.PubMed/NCBI View Article : Google Scholar

33 

Kwun IS, Cho YE, Lomeda RA, Shin HI, Choi JY, Kang YH and Beattie JH: Zinc deficiency suppresses matrix mineralization and retards osteogenesis transiently with catch-up possibly through Runx 2 modulation. Bone. 46:732–741. 2010.PubMed/NCBI View Article : Google Scholar

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Ren X, Wang Q, Liu C, Zhao Q, Zheng J, Tian K, Xu H and Mu Y: Osteogenic ability using porous hydroxyapatite scaffold‑based delivery of human placenta‑derived mesenchymal stem cells. Exp Ther Med 22: 1091, 2021.
APA
Ren, X., Wang, Q., Liu, C., Zhao, Q., Zheng, J., Tian, K. ... Mu, Y. (2021). Osteogenic ability using porous hydroxyapatite scaffold‑based delivery of human placenta‑derived mesenchymal stem cells. Experimental and Therapeutic Medicine, 22, 1091. https://doi.org/10.3892/etm.2021.10525
MLA
Ren, X., Wang, Q., Liu, C., Zhao, Q., Zheng, J., Tian, K., Xu, H., Mu, Y."Osteogenic ability using porous hydroxyapatite scaffold‑based delivery of human placenta‑derived mesenchymal stem cells". Experimental and Therapeutic Medicine 22.4 (2021): 1091.
Chicago
Ren, X., Wang, Q., Liu, C., Zhao, Q., Zheng, J., Tian, K., Xu, H., Mu, Y."Osteogenic ability using porous hydroxyapatite scaffold‑based delivery of human placenta‑derived mesenchymal stem cells". Experimental and Therapeutic Medicine 22, no. 4 (2021): 1091. https://doi.org/10.3892/etm.2021.10525
Copy and paste a formatted citation
x
Spandidos Publications style
Ren X, Wang Q, Liu C, Zhao Q, Zheng J, Tian K, Xu H and Mu Y: Osteogenic ability using porous hydroxyapatite scaffold‑based delivery of human placenta‑derived mesenchymal stem cells. Exp Ther Med 22: 1091, 2021.
APA
Ren, X., Wang, Q., Liu, C., Zhao, Q., Zheng, J., Tian, K. ... Mu, Y. (2021). Osteogenic ability using porous hydroxyapatite scaffold‑based delivery of human placenta‑derived mesenchymal stem cells. Experimental and Therapeutic Medicine, 22, 1091. https://doi.org/10.3892/etm.2021.10525
MLA
Ren, X., Wang, Q., Liu, C., Zhao, Q., Zheng, J., Tian, K., Xu, H., Mu, Y."Osteogenic ability using porous hydroxyapatite scaffold‑based delivery of human placenta‑derived mesenchymal stem cells". Experimental and Therapeutic Medicine 22.4 (2021): 1091.
Chicago
Ren, X., Wang, Q., Liu, C., Zhao, Q., Zheng, J., Tian, K., Xu, H., Mu, Y."Osteogenic ability using porous hydroxyapatite scaffold‑based delivery of human placenta‑derived mesenchymal stem cells". Experimental and Therapeutic Medicine 22, no. 4 (2021): 1091. https://doi.org/10.3892/etm.2021.10525
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team