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Berberine ameliorates nonalcoholic fatty liver disease by decreasing the liver lipid content via reversing the abnormal expression of MTTP and LDLR

  • Authors:
    • Ping Chen
    • Yusheng Li
    • Li Xiao
  • View Affiliations

  • Published online on: August 3, 2021     https://doi.org/10.3892/etm.2021.10543
  • Article Number: 1109
  • Copyright: © Chen et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The global incidence of nonalcoholic fatty liver disease (NAFLD) is increasing. The present study explored the effect and mechanism of berberine (BBR) on NAFLD in rats. Thirty‑five Sprague‑Dawley rats were randomly divided into the control and NAFLD groups, which were fed a normal diet or high‑fat diet, respectively, for 8 weeks. Hematoxylin and eosin staining was performed on liver tissues and establishment of the NAFLD model was confirmed by microscopy. NAFLD rats were subsequently randomly subdivided and treated with saline or BBR for 8 weeks. The liver wet weight of rats in each group was measured, the liver tissue structure was observed by microscopy, and alanine aminotransferase (ALT), aspartate aminotransferase (AST), total cholesterol (TC), triglyceride (TG), fasting blood glucose (FBG), low‑density lipoprotein (LDL) and high‑density lipoprotein (HDL) levels were detected using a semi‑automatic biochemical detector. Reverse transcription‑quantitative PCR and western blotting were performed to determine the mRNA and protein expression levels of microsomal triglyceride transfer protein (MTTP), apolipoprotein B and low‑density lipoprotein receptor (LDLR). Compared with the control group, the liver wet weight of the NAFLD rats was higher; the liver showed obvious fatty degeneration and liver TG levels increased significantly, as did serum levels of ALT, AST, TC, TG, FBG, HDL and LDL, while expression of MTTP and LDLR significantly decreased. Compared with the saline‑treated NAFLD rats, the BBR‑treated rats had reduced liver wet weight, improved liver steatosis and a significant decrease in liver TG levels, while ALT, AST, TC, TG, and LDL serum levels significantly decreased and MTTP levels were significantly upregulated. In conclusion, BBR treatment ameliorated the fatty liver induced by a high‑fat diet in rats. Furthermore, BBR reversed the abnormal expression of MTTP and LDLR in rats with high‑fat diet induced‑NAFLD. The present findings suggest that fatty liver could be improved by BBR administration, via reversing the abnormal expression of MTTP and LDLR and inhibiting lipid synthesis.
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October-2021
Volume 22 Issue 4

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Chen P, Li Y and Xiao L: Berberine ameliorates nonalcoholic fatty liver disease by decreasing the liver lipid content via reversing the abnormal expression of MTTP and LDLR. Exp Ther Med 22: 1109, 2021.
APA
Chen, P., Li, Y., & Xiao, L. (2021). Berberine ameliorates nonalcoholic fatty liver disease by decreasing the liver lipid content via reversing the abnormal expression of MTTP and LDLR. Experimental and Therapeutic Medicine, 22, 1109. https://doi.org/10.3892/etm.2021.10543
MLA
Chen, P., Li, Y., Xiao, L."Berberine ameliorates nonalcoholic fatty liver disease by decreasing the liver lipid content via reversing the abnormal expression of MTTP and LDLR". Experimental and Therapeutic Medicine 22.4 (2021): 1109.
Chicago
Chen, P., Li, Y., Xiao, L."Berberine ameliorates nonalcoholic fatty liver disease by decreasing the liver lipid content via reversing the abnormal expression of MTTP and LDLR". Experimental and Therapeutic Medicine 22, no. 4 (2021): 1109. https://doi.org/10.3892/etm.2021.10543