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Role of C5a and C5aR in doxorubicin‑induced cardiomyocyte senescence

  • Authors:
    • Yahui Wen
    • Feiyan Shen
    • Haibin Wu
  • View Affiliations / Copyright

    Affiliations: Medical Care Ward, Beijing Luhe Hospital, Capital Medical University, Beijing 101100, P.R. China, Department of Cardiology, QingPu District Central Hospital, Shanghai 201700, P.R. China, Department of Outpatients, Shenzhen Traditional Chinese Medicine Hospital, Guangdong Shenzhen Health Management Center, Shenzhen, Guangdong 518033, P.R. China
    Copyright: © Wen et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 1114
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    Published online on: August 4, 2021
       https://doi.org/10.3892/etm.2021.10548
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Abstract

Doxorubicin (DOX) is an efficacious antineoplastic drug; however, its use is limited due to its cardiotoxicity. Cardiomyocyte senescence is considered to be a key factor in the development of DOX‑related cardiomyopathy. Complement component 5a (C5a) and the C5a receptor (C5aR) have been reported to play a key role in the process of cellular senescence. However, to the best of our knowledge, the exact role of C5a and C5aR in cellular senescence in the heart remains largely unknown. Reverse transcription‑quantitative (RT‑q)PCR and western blot assays were used to analyze the expression levels of C5a and C5aR in H9c2 embryonic rat cardiomyocytes and AC16 human cardiomyocyte‑like cells. The cells were treated with DOX and a C5aR antagonist (C5aRA). The expression of TNF‑α and IFN‑γ was determined using ELISA and western blotting. The levels of reactive oxygen species (ROS) were also measured using ELISA. Cellular senescence was determined using senescence‑associated β‑galactosidase (SA‑β‑gal) staining and by analyzing the protein expression levels of p53, p16, p21 and insulin‑like growth factor‑binding protein 3 (IGFBP3). The expression levels of C5a and C5aR were found to be upregulated during the DOX‑induced senescence of H9c2 and AC16 cardiomyocytes. Treatment with C5aRA downregulated TNF‑α and IFN‑γ expression, in addition to ROS levels. Furthermore, C5aRA prevented DOX‑induced cellular senescence and decreased the levels of positive SA‑β‑gal staining in H9c2 and AC16 cardiomyocytes, in addition to downregulating the expression levels of p53, p16, p21 and IGFBP3. C5aRA also increased the telomere length and telomerase activity in H9c2 and AC16 cardiomyocytes following DOX stimulation. In conclusion, the findings of the present study indicated that C5a and C5aR may play a key role in cardiomyocyte senescence, and treatment with C5aRA may be an effective method for preventing DOX‑induced cardiomyocyte aging.
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Copy and paste a formatted citation
Spandidos Publications style
Wen Y, Shen F and Wu H: Role of C5a and C5aR in doxorubicin‑induced cardiomyocyte senescence. Exp Ther Med 22: 1114, 2021.
APA
Wen, Y., Shen, F., & Wu, H. (2021). Role of C5a and C5aR in doxorubicin‑induced cardiomyocyte senescence. Experimental and Therapeutic Medicine, 22, 1114. https://doi.org/10.3892/etm.2021.10548
MLA
Wen, Y., Shen, F., Wu, H."Role of C5a and C5aR in doxorubicin‑induced cardiomyocyte senescence". Experimental and Therapeutic Medicine 22.4 (2021): 1114.
Chicago
Wen, Y., Shen, F., Wu, H."Role of C5a and C5aR in doxorubicin‑induced cardiomyocyte senescence". Experimental and Therapeutic Medicine 22, no. 4 (2021): 1114. https://doi.org/10.3892/etm.2021.10548
Copy and paste a formatted citation
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Spandidos Publications style
Wen Y, Shen F and Wu H: Role of C5a and C5aR in doxorubicin‑induced cardiomyocyte senescence. Exp Ther Med 22: 1114, 2021.
APA
Wen, Y., Shen, F., & Wu, H. (2021). Role of C5a and C5aR in doxorubicin‑induced cardiomyocyte senescence. Experimental and Therapeutic Medicine, 22, 1114. https://doi.org/10.3892/etm.2021.10548
MLA
Wen, Y., Shen, F., Wu, H."Role of C5a and C5aR in doxorubicin‑induced cardiomyocyte senescence". Experimental and Therapeutic Medicine 22.4 (2021): 1114.
Chicago
Wen, Y., Shen, F., Wu, H."Role of C5a and C5aR in doxorubicin‑induced cardiomyocyte senescence". Experimental and Therapeutic Medicine 22, no. 4 (2021): 1114. https://doi.org/10.3892/etm.2021.10548
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