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Sodium tanshinone IIA sulfonate ameliorates cerebral ischemic injury through regulation of angiogenesis

  • Authors:
    • Jiazhen Xu
    • Pei Zhang
    • Yao Chen
    • Yulan Xu
    • Pengwei Luan
    • Yuying Zhu
    • Jiange Zhang
  • View Affiliations / Copyright

    Affiliations: Research Center of Chiral Drugs, Innovation Research Institute of Traditional Chinese Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, P.R. China, Institute of Drug Discovery and Development, School of Pharmaceutical Sciences, Zhengzhou University, Zhengzhou, Henan 450001, P.R. China
    Copyright: © Xu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 1122
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    Published online on: August 4, 2021
       https://doi.org/10.3892/etm.2021.10556
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Abstract

Vascular remodeling and neuroprotection are two major adaptable methods for treating ischemic stroke. Edaravone is a protective agent for the treatment of stroke and was used as a positive control in the present study. Sodium tanshinone IIA sulfonate (STS) has demonstrated therapeutic clinical effects in cerebral infarction in China, while its mechanisms of action in ischemic stroke have remained elusive. The angiogenesis and neuroprotective effects of STS were evaluated in a rat model induced by middle cerebral artery occlusion and 3 days of reperfusion. When used at the same dose, the magnitude of the therapeutic effect of STS was similar to that of edaravone in terms of decreased blood‑brain barrier damage as indicated by reduced Evans blue leakage, improved neurological deficits, alleviated cerebral edema and inhibition of histopathological changes caused by ischemia/reperfusion. The TUNEL assay demonstrated that the ability of STS to inhibit neuronal apoptosis was equivalent to that of edaravone. Immunofluorescence detection of CD31 and α‑smooth muscle actin indicated that the vascular density was significantly reduced in the vehicle group compared with that in the sham operation group, STS increased the microvessel density in the ischemic area. Furthermore, in the vehicle group the protein expression of vascular endothelial growth factor (VEGF) and VEGF receptor 2 (VEGFR) as determined by fluorescence microscopy and immunohistochemistry was significantly reduced compared with that in the sham group. However, STS promoted their expression compared to the vehicle group respectively, and increaed the mRNA expression of VEGF, VEGFR, CD31 and angiopoietin‑1 as determined by reverse transcription‑quantitative PCR, but these changes were not significant or not present for edaravone apart from Ang‑1. In conclusion, STS protected against ischemic brain injury by promoting angiogenesis in ischemic areas and inhibiting neuronal apoptosis. These results provide a potential treatment for stroke recovery.
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Spandidos Publications style
Xu J, Zhang P, Chen Y, Xu Y, Luan P, Zhu Y and Zhang J: Sodium tanshinone IIA sulfonate ameliorates cerebral ischemic injury through regulation of angiogenesis. Exp Ther Med 22: 1122, 2021.
APA
Xu, J., Zhang, P., Chen, Y., Xu, Y., Luan, P., Zhu, Y., & Zhang, J. (2021). Sodium tanshinone IIA sulfonate ameliorates cerebral ischemic injury through regulation of angiogenesis. Experimental and Therapeutic Medicine, 22, 1122. https://doi.org/10.3892/etm.2021.10556
MLA
Xu, J., Zhang, P., Chen, Y., Xu, Y., Luan, P., Zhu, Y., Zhang, J."Sodium tanshinone IIA sulfonate ameliorates cerebral ischemic injury through regulation of angiogenesis". Experimental and Therapeutic Medicine 22.4 (2021): 1122.
Chicago
Xu, J., Zhang, P., Chen, Y., Xu, Y., Luan, P., Zhu, Y., Zhang, J."Sodium tanshinone IIA sulfonate ameliorates cerebral ischemic injury through regulation of angiogenesis". Experimental and Therapeutic Medicine 22, no. 4 (2021): 1122. https://doi.org/10.3892/etm.2021.10556
Copy and paste a formatted citation
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Spandidos Publications style
Xu J, Zhang P, Chen Y, Xu Y, Luan P, Zhu Y and Zhang J: Sodium tanshinone IIA sulfonate ameliorates cerebral ischemic injury through regulation of angiogenesis. Exp Ther Med 22: 1122, 2021.
APA
Xu, J., Zhang, P., Chen, Y., Xu, Y., Luan, P., Zhu, Y., & Zhang, J. (2021). Sodium tanshinone IIA sulfonate ameliorates cerebral ischemic injury through regulation of angiogenesis. Experimental and Therapeutic Medicine, 22, 1122. https://doi.org/10.3892/etm.2021.10556
MLA
Xu, J., Zhang, P., Chen, Y., Xu, Y., Luan, P., Zhu, Y., Zhang, J."Sodium tanshinone IIA sulfonate ameliorates cerebral ischemic injury through regulation of angiogenesis". Experimental and Therapeutic Medicine 22.4 (2021): 1122.
Chicago
Xu, J., Zhang, P., Chen, Y., Xu, Y., Luan, P., Zhu, Y., Zhang, J."Sodium tanshinone IIA sulfonate ameliorates cerebral ischemic injury through regulation of angiogenesis". Experimental and Therapeutic Medicine 22, no. 4 (2021): 1122. https://doi.org/10.3892/etm.2021.10556
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