Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Experimental and Therapeutic Medicine
Join Editorial Board Propose a Special Issue
Print ISSN: 1792-0981 Online ISSN: 1792-1015
Journal Cover
October-2021 Volume 22 Issue 4

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
October-2021 Volume 22 Issue 4

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article Open Access

Decreased expression of miR‑3135b reduces sensitivity to 5‑fluorouracil in colorectal cancer by direct repression of PIM1

  • Authors:
    • Yan Wang
    • Xiaofeng Cui
    • Shurong Ma
    • Haishan Zhang
  • View Affiliations / Copyright

    Affiliations: Science Research Center, China‑Japan Union Hospital of Jilin University, Changchun, Jilin 130033, P.R. China, Department of Gastrointestinal Colorectal and Anal Surgery, China‑Japan Union Hospital of Jilin University, Changchun, Jilin 130033, P.R. China, Endoscopic Center, China‑Japan Union Hospital of Jilin University, Changchun, Jilin 130033, P.R. China
    Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 1151
    |
    Published online on: August 10, 2021
       https://doi.org/10.3892/etm.2021.10585
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

5‑Fluorouracil (5‑FU)‑based chemotherapy is the conventional treatment approach for patients with colorectal cancer (CRC). However, de novo and acquired resistance to 5‑FU are frequently observed during treatment, which eventually lead to patients succumbing to the disease. Accumulating data have revealed an association of CRC resistance to 5‑FU with aberrant expression of microRNAs (miRs). In the present study, Cell Counting Kit‑8 was performed to measure cell viability, flow cytometry was performed to detect cell apoptosis, reverse transcription‑quantitative PCR was conducted to measure proviral integration site for Moloney murine leukemia virus 1 (PIM1) and miR‑3135b expression, western blotting was conducted to measure PIM1 expression. Microarray data analysis indicated that the level of miR‑3135b expression was decreased in patients with recurrent CRC that were treated with 5‑FU when compared with non‑recurrent cases. Overexpression of miR‑3135b increased the sensitivity of CRC cells to 5‑FU treatment. Moreover, PIM1 was identified as a target gene of miR‑3135b using bioinformatics analysis, reverse transcription‑quantitative PCR and western blotting. The direct interaction between these two targets was confirmed by luciferase reporter assays. Notably, PIM1 overexpression compensated the effect of miR‑3135b in CRC cells. Furthermore, an inverse correlation between PIM1 mRNA expression levels and miR‑3135b expression was observed in clinical samples. Therefore, the present study identified miR‑3135b as a novel regulator of 5‑FU sensitivity in CRC.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

Figure 6

View References

1 

Torre LA, Bray F, Siegel RL, Ferlay J, Lortet-Tieulent J and Jemal A: Global cancer statistics, 2012. CA Cancer J Clin. 65:87–108. 2015.PubMed/NCBI View Article : Google Scholar

2 

Brody H: Colorectal cancer. Nature. 521(S1)2015.PubMed/NCBI View Article : Google Scholar

3 

Pourhoseingholi MA: Increased burden of colorectal cancer in Asia. World J Gastrointest Oncol. 4:68–70. 2012.PubMed/NCBI View Article : Google Scholar

4 

García-Osogobio S, Téllez-Ávila FI, Méndez N and Uribe-Esquivel M: Results of the first program of colorectal cancer screening in Mexico. Endoscopia. 27:59–63. 2015.

5 

Arnold D, Prager GW, Quintela A, Stein A, Vera SM, Mounedji N and Taieb J: Beyond second-line therapy in patients with metastatic colorectal cancer: A systematic review. Ann Oncol. 29:835–856. 2018.PubMed/NCBI View Article : Google Scholar

6 

Stintzing S: Management of colorectal cancer. F1000Prime Rep. 6(108)2014.PubMed/NCBI View Article : Google Scholar

7 

Vodenkova S, Buchler T, Cervena K, Veskrnova V, Vodicka P and Vymetalkova V: 5-fluorouracil and other fluoropyrimidines in colorectal cancer: Past, present and future. Pharmacol Ther. 206(107447)2020.PubMed/NCBI View Article : Google Scholar

8 

Liu C, Zhao Y, Wang J, Yang Y, Zhang Y, Qu X, Peng S, Yao Z, Zhao S, He B, et al: FoxO3 reverses 5-fluorouracil resistance in human colorectal cancer cells by inhibiting the Nrf2/TR1 signaling pathway. Cancer Lett. 470:29–42. 2020.PubMed/NCBI View Article : Google Scholar

9 

Bartel DP: MicroRNAs: Genomics, biogenesis, mechanism, and function. Cell. 116:281–297. 2004.PubMed/NCBI View Article : Google Scholar

10 

Wang XJ, Reyes JL, Chua NH and Gaasterland T: Prediction and identification of arabidopsis thaliana microRNAs and their mRNA targets. Genome Biol. 5(R65)2004.PubMed/NCBI View Article : Google Scholar

11 

Trang P, Weidhaas JB and Slack FJ: MicroRNAs as potential cancer therapeutics. Oncogene. 27 (Suppl 2):S52–S57. 2008.PubMed/NCBI View Article : Google Scholar

12 

Barisciano G, Colangelo T, Rosato V, Muccillo L, Taddei ML, Ippolito L, Chiarugi P, Galgani M, Bruzzaniti S, Matarese G, et al: miR-27a is a master regulator of metabolic reprogramming and chemoresistance in colorectal cancer. Br J Cancer. 122:1354–1366. 2020.PubMed/NCBI View Article : Google Scholar

13 

Liu T, Zhang X, Du L, Wang Y, Liu X, Tian H, Wang L, Li P, Zhao Y, Duan W, et al: Exosome-transmitted miR-128-3p increase chemosensitivity of oxaliplatin-resistant colorectal cancer. Mol Cancer. 18(43)2019.PubMed/NCBI View Article : Google Scholar

14 

Choi Y, Choi HS, Jeon WK, Kim BI, Park DI, Cho YK, Kim HJ, Park JH and Sohn CI: Optimal number of endoscopic biopsies in diagnosis of advanced gastric and colorectal cancer. J Korean Med Sci. 27:36–39. 2012.PubMed/NCBI View Article : Google Scholar

15 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001.PubMed/NCBI View Article : Google Scholar

16 

Xie P, Mo JL, Liu JH, Li X, Tan LM, Zhang W, Zhou HH and Liu ZQ: Pharmacogenomics of 5-fluorouracil in colorectal cancer: Review and update. Cell Oncol (Dordr). 43:989–1001. 2020.PubMed/NCBI View Article : Google Scholar

17 

Blondy S, David V, Verdier M, Mathonnet M, Perraud A and Christou N: 5-Fluorouracil resistance mechanisms in colorectal cancer: From classical pathways to promising processes. Cancer Sci. 111:3142–3154. 2020.PubMed/NCBI View Article : Google Scholar

18 

Zhao P, Ma YG, Zhao Y, Liu D, Dai ZJ, Yan CY and Guan HT: MicroRNA-552 deficiency mediates 5-fluorouracil resistance by targeting SMAD2 signaling in DNA-mismatch-repair-deficient colorectal cancer. Cancer Chemother Pharmacol. 84:427–439. 2019.PubMed/NCBI View Article : Google Scholar

19 

Liu G, Zhou J and Dong M: Down-regulation of miR-543 expression increases the sensitivity of colorectal cancer cells to 5-Fluorouracil through the PTEN/PI3K/AKT pathway. Biosci Rep. 39(BSR20190249)2019.PubMed/NCBI View Article : Google Scholar

20 

Dermani FK and Najafi R: miR-200c as a predictive biomarker for 5-Fluorouracil chemosensitivity in colorectal cancer. J Gastrointest Cancer. 49:102–103. 2018.PubMed/NCBI View Article : Google Scholar

21 

van Lohuizen M, Verbeek S, Krimpenfort P, Domen J, Saris C, Radaszkiewicz T and Berns A: Predisposition to lymphomagenesis in pim-1 transgenic mice: Cooperation with c-myc and N-myc in murine leukemia virus-induced tumors. Cell. 56:673–682. 1989.PubMed/NCBI View Article : Google Scholar

22 

Herzog S, Fink MA, Weitmann K, Friedel C, Hadlich S, Langner S, Kindermann K, Holm T, Böhm A, Eskilsson E, et al: Pim1 kinase is upregulated in glioblastoma multiforme and mediates tumor cell survival. Neuro Oncol. 17:223–242. 2015.PubMed/NCBI View Article : Google Scholar

23 

Jiang R, Wang X, Jin Z and Li K: Association of nuclear PIM1 expression with lymph node metastasis and poor prognosis in patients with lung adenocarcinoma and squamous cell carcinoma. J Cancer. 7:324–334. 2016.PubMed/NCBI View Article : Google Scholar

24 

Zhang M, Liu T, Sun H, Weng W, Zhang Q, Liu C, Han Y and Sheng W: Pim1 supports human colorectal cancer growth during glucose deprivation by enhancing the warburg effect. Cancer Sci. 109:1468–1479. 2018.PubMed/NCBI View Article : Google Scholar

25 

Gu JJ, Wang Z, Reeves R and Magnuson NS: PIM1 phosphorylates and negatively regulates ASK1-mediated apoptosis. Oncogene. 28:4261–4271. 2009.PubMed/NCBI View Article : Google Scholar

26 

Li Q, Chen L, Luo C, ChenYan Ge J, Zhu Z, Wang K, Yu X, Lei J, Liu T, et al: TAB3 upregulates PIM1 expression by directly activating the TAK1-STAT3 complex to promote colorectal cancer growth. Exp Cell Res. 391(111975)2020.PubMed/NCBI View Article : Google Scholar

27 

Braso-Maristany F, Filosto S, Catchpole S, Marlow R, Quist J, Francesch-Domenech E, Plumb DA, Zakka L, Gazinska P, Liccardi G, et al: PIM1 kinase regulates cell death, tumor growth and chemotherapy response in triple-negative breast cancer. Nat Med. 22:1303–1313. 2016.PubMed/NCBI View Article : Google Scholar

28 

Blanco FF, Jimbo M, Wulfkuhle J, Gallagher I, Deng J, Enyenihi L, Meisner-Kober N, Londin E, Rigoutsos I, Sawicki JA, et al: The mRNA-binding protein HuR promotes hypoxia-induced chemoresistance through posttranscriptional regulation of the proto-oncogene PIM1 in pancreatic cancer cells. Oncogene. 35:2529–2541. 2016.PubMed/NCBI View Article : Google Scholar

29 

Weirauch U, Beckmann N, Thomas M, Grünweller A, Huber K, Bracher F, Hartmann RK and Aigner A: Functional role and therapeutic potential of the pim-1 kinase in colon carcinoma. Neoplasia. 15:783–794. 2013.PubMed/NCBI View Article : Google Scholar

30 

Núñez-Olvera SI, Chávez-Munguía B, Del Rocío Terrones-Gurrola MC, Marchat LA, Puente-Rivera J, Ruíz-García E, Campos-Parra AD, Vázquez-Calzada C, Lizárraga-Verdugo ER, Ramos-Payán R, et al: A novel protective role for microRNA-3135b in golgi apparatus fragmentation induced by chemotherapy via GOLPH3/AKT1/mTOR axis in colorectal cancer cells. Sci Rep. 10(10555)2020.PubMed/NCBI View Article : Google Scholar

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Wang Y, Cui X, Ma S and Zhang H: Decreased expression of miR‑3135b reduces sensitivity to 5‑fluorouracil in colorectal cancer by direct repression of PIM1. Exp Ther Med 22: 1151, 2021.
APA
Wang, Y., Cui, X., Ma, S., & Zhang, H. (2021). Decreased expression of miR‑3135b reduces sensitivity to 5‑fluorouracil in colorectal cancer by direct repression of PIM1. Experimental and Therapeutic Medicine, 22, 1151. https://doi.org/10.3892/etm.2021.10585
MLA
Wang, Y., Cui, X., Ma, S., Zhang, H."Decreased expression of miR‑3135b reduces sensitivity to 5‑fluorouracil in colorectal cancer by direct repression of PIM1". Experimental and Therapeutic Medicine 22.4 (2021): 1151.
Chicago
Wang, Y., Cui, X., Ma, S., Zhang, H."Decreased expression of miR‑3135b reduces sensitivity to 5‑fluorouracil in colorectal cancer by direct repression of PIM1". Experimental and Therapeutic Medicine 22, no. 4 (2021): 1151. https://doi.org/10.3892/etm.2021.10585
Copy and paste a formatted citation
x
Spandidos Publications style
Wang Y, Cui X, Ma S and Zhang H: Decreased expression of miR‑3135b reduces sensitivity to 5‑fluorouracil in colorectal cancer by direct repression of PIM1. Exp Ther Med 22: 1151, 2021.
APA
Wang, Y., Cui, X., Ma, S., & Zhang, H. (2021). Decreased expression of miR‑3135b reduces sensitivity to 5‑fluorouracil in colorectal cancer by direct repression of PIM1. Experimental and Therapeutic Medicine, 22, 1151. https://doi.org/10.3892/etm.2021.10585
MLA
Wang, Y., Cui, X., Ma, S., Zhang, H."Decreased expression of miR‑3135b reduces sensitivity to 5‑fluorouracil in colorectal cancer by direct repression of PIM1". Experimental and Therapeutic Medicine 22.4 (2021): 1151.
Chicago
Wang, Y., Cui, X., Ma, S., Zhang, H."Decreased expression of miR‑3135b reduces sensitivity to 5‑fluorouracil in colorectal cancer by direct repression of PIM1". Experimental and Therapeutic Medicine 22, no. 4 (2021): 1151. https://doi.org/10.3892/etm.2021.10585
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team