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circASS1 overexpression inhibits the proliferation, invasion and migration of colorectal cancer cells by regulating the miR‑1269a/VASH1 axis

  • Authors:
    • Hai-Lin Xiong
    • Xiao-Hua Zhong
    • Xiao-Hong Guo
    • Hao-Jie Liao
    • Xia Yuan
  • View Affiliations / Copyright

    Affiliations: Department of Medical Oncology, Huizhou Municipal Central Hospital of Guangdong Province, Huizhou, Guangdong 516000, P.R. China, Department of Gastroenterological Surgery, Huizhou Municipal Central Hospital of Guangdong Province, Huizhou, Guangdong 516000, P.R. China
    Copyright: © Xiong et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 1155
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    Published online on: August 10, 2021
       https://doi.org/10.3892/etm.2021.10589
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Abstract

Colorectal cancer (CRC), the third most common cancer worldwide, poses a threat to human life. However, its underlying mechanism is unclear and no satisfactory treatment is available. The present study aimed to investigate the role of circular RNA argininosuccinate synthase 1 (circASS1) in CRC cells and tissues to identify the potential mechanism underlying the pathogenesis of CRC. The expression of circASS1 in CRC cells and tissues was determined by reverse transcription‑quantitative PCR. Following circASS1 overexpression in HT29 cells, cell viability, colony formation and apoptosis were measured using MTT, colony formation and TUNEL assays, respectively. Cell invasion and migration were also assessed. After confirming the associations among circASS1, microRNA (miR)‑1269a and vasohibin 1 (VASH1), the characteristics of the HT29 cell line were assessed by performing the aforementioned assays. circASS1 expression was decreased in CRC cells and tissues, and circASS1 overexpression suppressed CRC cell proliferation, invasion and migration. circASS1 adsorbed miR‑1269a and regulated its expression, and VASH1 was a target protein of miR‑1269a. circASS1 overexpression decreased cell proliferation, invasion and migration, but enhanced cell apoptosis in HT29 cells, which was reversed by co‑transfection with miR‑1269a mimic or short hairpin RNA‑VASH1. In conclusion, circASS1 overexpression inhibited CRC cell proliferation, invasion and migration by regulating miR‑1269a/VASH1, which indicated a potential molecular mechanism underlying the pathogenesis of CRC.
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Copy and paste a formatted citation
Spandidos Publications style
Xiong H, Zhong X, Guo X, Liao H and Yuan X: circASS1 overexpression inhibits the proliferation, invasion and migration of colorectal cancer cells by regulating the miR‑1269a/VASH1 axis. Exp Ther Med 22: 1155, 2021.
APA
Xiong, H., Zhong, X., Guo, X., Liao, H., & Yuan, X. (2021). circASS1 overexpression inhibits the proliferation, invasion and migration of colorectal cancer cells by regulating the miR‑1269a/VASH1 axis. Experimental and Therapeutic Medicine, 22, 1155. https://doi.org/10.3892/etm.2021.10589
MLA
Xiong, H., Zhong, X., Guo, X., Liao, H., Yuan, X."circASS1 overexpression inhibits the proliferation, invasion and migration of colorectal cancer cells by regulating the miR‑1269a/VASH1 axis". Experimental and Therapeutic Medicine 22.4 (2021): 1155.
Chicago
Xiong, H., Zhong, X., Guo, X., Liao, H., Yuan, X."circASS1 overexpression inhibits the proliferation, invasion and migration of colorectal cancer cells by regulating the miR‑1269a/VASH1 axis". Experimental and Therapeutic Medicine 22, no. 4 (2021): 1155. https://doi.org/10.3892/etm.2021.10589
Copy and paste a formatted citation
x
Spandidos Publications style
Xiong H, Zhong X, Guo X, Liao H and Yuan X: circASS1 overexpression inhibits the proliferation, invasion and migration of colorectal cancer cells by regulating the miR‑1269a/VASH1 axis. Exp Ther Med 22: 1155, 2021.
APA
Xiong, H., Zhong, X., Guo, X., Liao, H., & Yuan, X. (2021). circASS1 overexpression inhibits the proliferation, invasion and migration of colorectal cancer cells by regulating the miR‑1269a/VASH1 axis. Experimental and Therapeutic Medicine, 22, 1155. https://doi.org/10.3892/etm.2021.10589
MLA
Xiong, H., Zhong, X., Guo, X., Liao, H., Yuan, X."circASS1 overexpression inhibits the proliferation, invasion and migration of colorectal cancer cells by regulating the miR‑1269a/VASH1 axis". Experimental and Therapeutic Medicine 22.4 (2021): 1155.
Chicago
Xiong, H., Zhong, X., Guo, X., Liao, H., Yuan, X."circASS1 overexpression inhibits the proliferation, invasion and migration of colorectal cancer cells by regulating the miR‑1269a/VASH1 axis". Experimental and Therapeutic Medicine 22, no. 4 (2021): 1155. https://doi.org/10.3892/etm.2021.10589
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