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MicroRNA‑124‑5p delays the progression of cerebral aneurysm by regulating FoxO1

  • Authors:
    • Ru-Ke Wang
    • Yuan-Yuan Sun
    • Guang-You Li
    • Hua-Tang Yang
    • Xiu-Jie Liu
    • Ke-Feng Li
    • Xu Zhu
    • Guo-Yuan Yu
  • View Affiliations / Copyright

    Affiliations: Section 2, Department of Neurosurgery, Handan Central Hospital, Handan, Hebei 056001, P.R. China, CT Room, Handan First Hospital, Handan, Hebei 056002, P.R. China
    Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 1172
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    Published online on: August 13, 2021
       https://doi.org/10.3892/etm.2021.10606
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Abstract

Cerebral aneurysm (CA) is a common brain disease, and the development of cerebral aneurysm is driven by inflammation and hemodynamic stress. MicroRNA (miR)‑124‑5p is reported to be associated with inflammatory response in brain disease such as cerebral ischemia‑reperfusion injury. However, the function and molecular mechanism of miR‑124‑5p in CA are not clear, thus, the effects of miR‑124‑5p on inflammatory response in CA were explored. Firstly, the expression of miR‑124‑5p in the peripheral blood of patients with CA and the control group was detected by reverse transcription‑quantitative PCR. Then, the human umbilical vein endothelial cells (HUVECs) were used as an in vitro model system and stimulated with interleukin (IL)‑1β to simulate the inflammatory environment of CA, and the expression of miR‑124‑5p was detected. Next, the effect of miR‑124‑5p on the migration and invasion of HUVECs was detected using Transwell assays. Meanwhile, the function of miR‑124‑5p on various inflammatory factors was determined by western blotting and enzyme‑linked immunosorbent assay (ELISA). Next, the TargetScan website was used to predict FoxO1 as a target gene of miR‑124‑5p, and this target association was validated by double luciferase reporter assay and western blotting. Finally, the interaction of miR‑124‑5p with FoxO1 in CA was measured by Transwell western blotting and ELISA assays. The results showed that the expression level of miR‑124‑5p in the peripheral blood of patients with CA was lower compared with that of control group, and the miR‑124‑5p in HUVECs stimulated by IL‑1β was less compared with that in normal HUVECs. Besides, miR‑124‑5p could inhibit the migration and invasion abilities of HUVECs and the release of inflammatory factors. Additionally, the overexpression of miR‑124‑5p was able to inhibit the expression of FoxO1. miR‑124‑5p‑inhibitor promoted the migration and invasion of HUVECs, as well as inflammatory response, which was weakened following the introduction of FoxO1 small interfering RNA. Overall, the present study demonstrated that miR‑124‑5p could prevent the occurrence and development of cerebral aneurysm by downregulating the expression of FoxO1.
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Copy and paste a formatted citation
Spandidos Publications style
Wang R, Sun Y, Li G, Yang H, Liu X, Li K, Zhu X and Yu G: MicroRNA‑124‑5p delays the progression of cerebral aneurysm by regulating FoxO1. Exp Ther Med 22: 1172, 2021.
APA
Wang, R., Sun, Y., Li, G., Yang, H., Liu, X., Li, K. ... Yu, G. (2021). MicroRNA‑124‑5p delays the progression of cerebral aneurysm by regulating FoxO1. Experimental and Therapeutic Medicine, 22, 1172. https://doi.org/10.3892/etm.2021.10606
MLA
Wang, R., Sun, Y., Li, G., Yang, H., Liu, X., Li, K., Zhu, X., Yu, G."MicroRNA‑124‑5p delays the progression of cerebral aneurysm by regulating FoxO1". Experimental and Therapeutic Medicine 22.4 (2021): 1172.
Chicago
Wang, R., Sun, Y., Li, G., Yang, H., Liu, X., Li, K., Zhu, X., Yu, G."MicroRNA‑124‑5p delays the progression of cerebral aneurysm by regulating FoxO1". Experimental and Therapeutic Medicine 22, no. 4 (2021): 1172. https://doi.org/10.3892/etm.2021.10606
Copy and paste a formatted citation
x
Spandidos Publications style
Wang R, Sun Y, Li G, Yang H, Liu X, Li K, Zhu X and Yu G: MicroRNA‑124‑5p delays the progression of cerebral aneurysm by regulating FoxO1. Exp Ther Med 22: 1172, 2021.
APA
Wang, R., Sun, Y., Li, G., Yang, H., Liu, X., Li, K. ... Yu, G. (2021). MicroRNA‑124‑5p delays the progression of cerebral aneurysm by regulating FoxO1. Experimental and Therapeutic Medicine, 22, 1172. https://doi.org/10.3892/etm.2021.10606
MLA
Wang, R., Sun, Y., Li, G., Yang, H., Liu, X., Li, K., Zhu, X., Yu, G."MicroRNA‑124‑5p delays the progression of cerebral aneurysm by regulating FoxO1". Experimental and Therapeutic Medicine 22.4 (2021): 1172.
Chicago
Wang, R., Sun, Y., Li, G., Yang, H., Liu, X., Li, K., Zhu, X., Yu, G."MicroRNA‑124‑5p delays the progression of cerebral aneurysm by regulating FoxO1". Experimental and Therapeutic Medicine 22, no. 4 (2021): 1172. https://doi.org/10.3892/etm.2021.10606
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