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Cyclophosphamide inhibits the progression of Meniere's disease by reducing the generation of circulating immune complex

  • Authors:
    • Shu Zhang
    • Yulin Gong
    • Yu Liang
    • Boqian Wang
    • Wei Gao
    • Qianyun Xu
  • View Affiliations / Copyright

    Affiliations: Department of Otolaryngology, The Affiliated Hospital of Inner Mongolia Medical University, Hohhot, Inner Mongolia 010110, P.R. China, Department of Rheumatology and Immunology, The Affiliated Hospital of Inner Mongolia Medical University, Hohhot, Inner Mongolia 010110, P.R. China, Department of Computer Information and Network Management Center, The Affiliated Hospital of Inner Mongolia Medical University, Hohhot, Inner Mongolia 010110, P.R. China
    Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 1177
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    Published online on: August 13, 2021
       https://doi.org/10.3892/etm.2021.10611
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Abstract

Endolymphatic hydrops is a characteristic pathological manifestation of Meniere's disease (MD) that has been previously associated with autoimmunity. Interest in the circulating immune complex (CIC) has increased due to its reported role in the occurrence of MD. The present study aimed to investigate the potential value of serum CIC concentration in the diagnosis of MD and the therapeutic potential of cyclophosphamide (CTX) for the treatment of MD. In the present study, guinea pigs were immunized with isologous crude inner ear antigens to establish an autoimmune MD model. Pure tone audiometry, Vestibular‑evoked myogenic potential test, electrocochleography test and auditory brainstem response was applied in this study for assessing the severity of MD in guinea pigs. ELISA was applied to measure CIC, tumor necrosis factor α (TNF‑α) and heat shock protein 70 (HSP70) expression levels in the serum samples of different groups of patients. Western blotting was applied to detect the protein expression of HSP70 in inner ear tissues in guinea pigs. Hematoxylin and eosin staining was applied to visualize the spiral ganglions in spiral ganglions models. CIC expression in the inner ear was detected by immunohistochemistry. In vivo experiments were performed to confirm the therapeutic effects of CTX in MD. Serum concentrations of CIC, TNF‑α and HSP70 were found to be significantly higher in patients with MD, which were also associated with increases in hearing classification and the severity of endolymphatic hydrops. Using a guinea pig MD model, ELISA results revealed significantly increased serum CIC, TNF‑α and HSP70 concentrations compared with those in the control group. ABR results showed that the thresholds in the CTX group were notably decreased compared with that in the dexamethasone group, whereas CIC concentrations in the serum were reduced following dexamethasone and CTX treatments compared with those after saline treatment. In the inner ear tissues, the CIC concentration in CTX group was lower than that in the dexamethasone group. Similarly, reductions in HSP70 and TNF‑α concentrations was also observed in a similar manner. Immunohistochemistry staining found notably lower CIC deposition in the inner ear tissues following CTX treatment than that in dexamethasone group. Taken together, higher CIC expression can be used as a biomarker for MD diagnosis. The efficacy of CTX in MD was found to be higher compared with that in dexamethasone, which may be associated with the effective inhibition of CIC, HSP70 and TNF‑α generation.
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Copy and paste a formatted citation
Spandidos Publications style
Zhang S, Gong Y, Liang Y, Wang B, Gao W and Xu Q: Cyclophosphamide inhibits the progression of Meniere's disease by reducing the generation of circulating immune complex. Exp Ther Med 22: 1177, 2021.
APA
Zhang, S., Gong, Y., Liang, Y., Wang, B., Gao, W., & Xu, Q. (2021). Cyclophosphamide inhibits the progression of Meniere's disease by reducing the generation of circulating immune complex. Experimental and Therapeutic Medicine, 22, 1177. https://doi.org/10.3892/etm.2021.10611
MLA
Zhang, S., Gong, Y., Liang, Y., Wang, B., Gao, W., Xu, Q."Cyclophosphamide inhibits the progression of Meniere's disease by reducing the generation of circulating immune complex". Experimental and Therapeutic Medicine 22.4 (2021): 1177.
Chicago
Zhang, S., Gong, Y., Liang, Y., Wang, B., Gao, W., Xu, Q."Cyclophosphamide inhibits the progression of Meniere's disease by reducing the generation of circulating immune complex". Experimental and Therapeutic Medicine 22, no. 4 (2021): 1177. https://doi.org/10.3892/etm.2021.10611
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang S, Gong Y, Liang Y, Wang B, Gao W and Xu Q: Cyclophosphamide inhibits the progression of Meniere's disease by reducing the generation of circulating immune complex. Exp Ther Med 22: 1177, 2021.
APA
Zhang, S., Gong, Y., Liang, Y., Wang, B., Gao, W., & Xu, Q. (2021). Cyclophosphamide inhibits the progression of Meniere's disease by reducing the generation of circulating immune complex. Experimental and Therapeutic Medicine, 22, 1177. https://doi.org/10.3892/etm.2021.10611
MLA
Zhang, S., Gong, Y., Liang, Y., Wang, B., Gao, W., Xu, Q."Cyclophosphamide inhibits the progression of Meniere's disease by reducing the generation of circulating immune complex". Experimental and Therapeutic Medicine 22.4 (2021): 1177.
Chicago
Zhang, S., Gong, Y., Liang, Y., Wang, B., Gao, W., Xu, Q."Cyclophosphamide inhibits the progression of Meniere's disease by reducing the generation of circulating immune complex". Experimental and Therapeutic Medicine 22, no. 4 (2021): 1177. https://doi.org/10.3892/etm.2021.10611
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