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Ononin alleviates H2O2‑induced cardiomyocyte apoptosis and improves cardiac function by activating the AMPK/mTOR/autophagy pathway

  • Authors:
    • Rongrong Pan
    • Qin Zhuang
    • Jiangtin Wang
  • View Affiliations / Copyright

    Affiliations: Department of Cardiology, Cixi People's Hospital, Wenzhou Medical University, Cixi, Zhejiang 315300, P.R. China, Department of Cardiology, Zhejiang Hospital, Hangzhou, Zhejiang 310013, P.R. China
    Copyright: © Pan et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 1307
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    Published online on: September 16, 2021
       https://doi.org/10.3892/etm.2021.10742
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Abstract

Ononin (ON) is an isoflavone with numerous reported bioactivities, including anti‑oxidative, anti‑inflammatory and neuroprotective effects. Autophagy is a critical homeostatic process in the body that has been reported to closely associate with the apoptotic processes of cardiomyocytes. Using flow cytometry, western blotting, echocardiography and Masson's staining, the present study investigated the effects of ON on H2O2‑induced cardiomyocyte apoptosis and myocardial infarction, in addition to any potential underlying molecular mechanisms. H2O2 treatment reliably induced apoptosis in H9C2 cells. The anti‑apoptotic effects of ON were revealed by flow cytometry results and by the downregulation of cleaved‑caspase 3. Further investigations indicated that ON may alleviate apoptosis by enhancing autophagy, as evidenced by increased microtubule‑associated proteins 1A/1B light chain 3B expression and p62 degradation. Activation of the 5' AMP‑activated protein kinase (AMPK)/mTOR pathway was observed after ON administration following H2O2‑induced cardiomyocyte injury. However, these anti‑apoptotic effects mediated by ON were lost after autophagy inhibition by chloroquine or AMPK inhibition by Compound C. Finally, the protective effects of ON on cardiomyocytes in vitro could also be observed in vivo. A myocardial infarction model was established by ligating the left anterior descending branch of the rat heart. Using echocardiography and Masson's staining, ON was shown to increase the ejection fraction and decrease cardiac fibrosis in rats with myocardial infarction. These results suggest that ON exerts cardioprotective effects by improving autophagy via the AMPK/mTOR signaling pathway.
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Copy and paste a formatted citation
Spandidos Publications style
Pan R, Zhuang Q and Wang J: Ononin alleviates H<sub>2</sub>O<sub>2</sub>‑induced cardiomyocyte apoptosis and improves cardiac function by activating the AMPK/mTOR/autophagy pathway. Exp Ther Med 22: 1307, 2021.
APA
Pan, R., Zhuang, Q., & Wang, J. (2021). Ononin alleviates H<sub>2</sub>O<sub>2</sub>‑induced cardiomyocyte apoptosis and improves cardiac function by activating the AMPK/mTOR/autophagy pathway. Experimental and Therapeutic Medicine, 22, 1307. https://doi.org/10.3892/etm.2021.10742
MLA
Pan, R., Zhuang, Q., Wang, J."Ononin alleviates H<sub>2</sub>O<sub>2</sub>‑induced cardiomyocyte apoptosis and improves cardiac function by activating the AMPK/mTOR/autophagy pathway". Experimental and Therapeutic Medicine 22.5 (2021): 1307.
Chicago
Pan, R., Zhuang, Q., Wang, J."Ononin alleviates H<sub>2</sub>O<sub>2</sub>‑induced cardiomyocyte apoptosis and improves cardiac function by activating the AMPK/mTOR/autophagy pathway". Experimental and Therapeutic Medicine 22, no. 5 (2021): 1307. https://doi.org/10.3892/etm.2021.10742
Copy and paste a formatted citation
x
Spandidos Publications style
Pan R, Zhuang Q and Wang J: Ononin alleviates H<sub>2</sub>O<sub>2</sub>‑induced cardiomyocyte apoptosis and improves cardiac function by activating the AMPK/mTOR/autophagy pathway. Exp Ther Med 22: 1307, 2021.
APA
Pan, R., Zhuang, Q., & Wang, J. (2021). Ononin alleviates H<sub>2</sub>O<sub>2</sub>‑induced cardiomyocyte apoptosis and improves cardiac function by activating the AMPK/mTOR/autophagy pathway. Experimental and Therapeutic Medicine, 22, 1307. https://doi.org/10.3892/etm.2021.10742
MLA
Pan, R., Zhuang, Q., Wang, J."Ononin alleviates H<sub>2</sub>O<sub>2</sub>‑induced cardiomyocyte apoptosis and improves cardiac function by activating the AMPK/mTOR/autophagy pathway". Experimental and Therapeutic Medicine 22.5 (2021): 1307.
Chicago
Pan, R., Zhuang, Q., Wang, J."Ononin alleviates H<sub>2</sub>O<sub>2</sub>‑induced cardiomyocyte apoptosis and improves cardiac function by activating the AMPK/mTOR/autophagy pathway". Experimental and Therapeutic Medicine 22, no. 5 (2021): 1307. https://doi.org/10.3892/etm.2021.10742
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