Open Access

Effects of Psoralea corylifolia L. seed extract on AGEs‑induced cell proliferation and fibrotic factor expression in mesangial cells

  • Authors:
    • Hyunah Chung
    • Eunhui Seo
    • Hee-Sook Jun
  • View Affiliations

  • Published online on: September 20, 2021     https://doi.org/10.3892/etm.2021.10767
  • Article Number: 1332
  • Copyright: © Chung et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Diabetic nephropathy is a microvascular complication of diabetes that is characterized by mesangial expansion and thickening of the glomerular basement membrane. The production of advanced glycation end products (AGEs) increases in diabetic patients. Activation of the receptor of AGE (RAGE) signaling pathway induces mesangial expansion via the reactive oxygen species (ROS)‑mediated production of pro‑inflammatory and extracellular matrix molecules. The Psoralea corylifolia L. seed (PCS) is a widely used herbal medicine with various biological activities. The current study investigated the effect of PCS extract on mesangial cell proliferation and the RAGE signaling pathway in SV40 MES 13 cells. SV40 MES 13 cells were harvested after treatment with various concentrations of PCS extract at 10 µg/ml AGEs for 24 h. The results revealed that the PCS extract inhibited AGEs‑induced mesangial cell proliferation and cyclin protein expression in a concentration‑dependent manner. In addition, the AGEs‑induced expression of fibrotic factors, such as transforming growth factor β, fibronectin and collagen, was reduced in mesangial cells after exposure to the PCS extract. The PCS extract also reduced RAGE expression and inhibited the expression of its downstream signaling pathways, such as NADPH oxidase, intracellular ROS and phospho‑NF‑κB. In conclusion, the data suggested that the PCS extract attenuated AGEs‑induced renal mesangial cell proliferation and fibrosis via the suppression of oxidative stress and the downregulation of inflammatory and fibrotic factor expression.
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November-2021
Volume 22 Issue 5

Print ISSN: 1792-0981
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Spandidos Publications style
Chung H, Seo E and Jun H: Effects of <em>Psoralea corylifolia </em>L. seed extract on AGEs‑induced cell proliferation and fibrotic factor expression in mesangial cells. Exp Ther Med 22: 1332, 2021
APA
Chung, H., Seo, E., & Jun, H. (2021). Effects of <em>Psoralea corylifolia </em>L. seed extract on AGEs‑induced cell proliferation and fibrotic factor expression in mesangial cells. Experimental and Therapeutic Medicine, 22, 1332. https://doi.org/10.3892/etm.2021.10767
MLA
Chung, H., Seo, E., Jun, H."Effects of <em>Psoralea corylifolia </em>L. seed extract on AGEs‑induced cell proliferation and fibrotic factor expression in mesangial cells". Experimental and Therapeutic Medicine 22.5 (2021): 1332.
Chicago
Chung, H., Seo, E., Jun, H."Effects of <em>Psoralea corylifolia </em>L. seed extract on AGEs‑induced cell proliferation and fibrotic factor expression in mesangial cells". Experimental and Therapeutic Medicine 22, no. 5 (2021): 1332. https://doi.org/10.3892/etm.2021.10767