Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Experimental and Therapeutic Medicine
Join Editorial Board Propose a Special Issue
Print ISSN: 1792-0981 Online ISSN: 1792-1015
Journal Cover
November-2021 Volume 22 Issue 5

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
November-2021 Volume 22 Issue 5

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article Open Access

MEX3A knockdown inhibits the tumorigenesis of colorectal cancer via modulating CDK2 expression

  • Authors:
    • Xin Zhou
    • Shaojie Li
    • Tiexiang Ma
    • Jian Zeng
    • Huanyu Li
    • Xiang Liu
    • Feng Li
    • Bin Jiang
    • Ming Zhao
    • Zhuo Liu
    • Yiyu Qin
  • View Affiliations / Copyright

    Affiliations: Department of General Surgery, Xiangtan Central Hospital, Xiangtan, Hunan 411100, P.R. China, Clinical Medical College, Follow-up Research Center, Jiangsu Vocational College of Medicine, Yancheng, Jiangsu 224005, P.R. China
    Copyright: © Zhou et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 1343
    |
    Published online on: September 22, 2021
       https://doi.org/10.3892/etm.2021.10778
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Colorectal cancer (CRC) is a malignant tumor of the gastrointestinal tract and a leading cause of cancer‑associated mortality worldwide. Mex‑3 RNA binding family member A (MEX3A) promotes the progression of multiple types of cancer, including ovarian and cervical cancer. However, to the best of our knowledge, the role of MEX3A in CRC is not completely understood. Therefore, the present study aimed to investigate the function of MEX3A in CRC. The mRNA and protein expression levels of MEX3A in CRC cells were analyzed using reverse transcription‑quantitative PCR and western blotting, respectively. Cell Counting Kit‑8 assays were used to measure cell viability. Cell apoptosis and cell cycle distribution were detected via flow cytometry, and CRC cell invasion was analyzed by performing Transwell assays. Moreover, the mitochondrial membrane potential in CRC cells was measured via JC‑1 staining. The results of the present study revealed that the expression levels of MEX3A were upregulated in CRC tissues compared with adjacent healthy tissues. MEX3A knockdown notably inhibited CRC cell viability, and induced apoptosis and mitochondrial injury. In addition, MEX3A knockdown markedly induced G1 phase cell cycle arrest in CRC cells via downregulating CDK2 expression. In conclusion, the findings of the present study suggested that MEX3A knockdown may inhibit the tumorigenesis of CRC cells by regulating CDK2 expression. Therefore, MEX3A may serve as a novel target for CRC treatment.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

Figure 6

View References

1 

Parine NR, Azzam NA, Shaik J, Aljebreen AM, Alharbi O, Almadi MA, Alanazi M and Khan Z: Genetic variants in the WNT signaling pathway are protectively associated with colorectal cancer in a Saudi population. Saudi J Biol Sci. 26:286–293. 2019.PubMed/NCBI View Article : Google Scholar

2 

Feroldi F, Verlaan M, Knaus H, Davidoiu V, Vugts DJ, van Dongen GA, Molthoff CF and de Boer JF: High resolution combined molecular and structural optical imaging of colorectal cancer in a xenograft mouse model. Biomed Opt Express. 9:6186–6204. 2018.PubMed/NCBI View Article : Google Scholar

3 

Gala de Pablo J, Armistead FJ, Peyman SA, Bonthron D, Lones M, Smith S and Evans SD: Biochemical fingerprint of colorectal cancer cell lines using label-free live single-cell Raman spectroscopy. J Raman Spectrosc. 49:1323–1332. 2018.PubMed/NCBI View Article : Google Scholar

4 

Panzeri V, Manni I, Capone A, Naro C, Sacconi A, Di Agostino S, de Latouliere L, Montori A, Pilozzi E, Piaggio G, et al: The RNA-binding protein MEX3A is a prognostic factor and regulator of resistance to gemcitabine in pancreatic ductal adenocarcinoma. Mol Oncol. 15:579–595. 2021.PubMed/NCBI View Article : Google Scholar

5 

Naef V, De Sarlo M, Testa G, Corsinovi D, Azzarelli R, Borello U and Ori M: The stemness gene Mex3A is a key regulator of neuroblast proliferation during neurogenesis. Front Cell Dev Biol. 8(549533)2020.PubMed/NCBI View Article : Google Scholar

6 

Liang J, Li H, Han J, Jiang J, Wang J, Li Y, Feng Z, Zhao R, Sun Z, Lv B, et al: Mex3a interacts with LAMA2 to promote lung adenocarcinoma metastasis via PI3K/AKT pathway. Cell Death Dis. 11(614)2020.PubMed/NCBI View Article : Google Scholar

7 

Jiang S, Meng L, Chen X, Liu H, Zhang J, Chen F, Zheng J, Liu H, Wang F, Hu J, et al: MEX3A promotes triple negative breast cancer proliferation and migration via the PI3K/AKT signaling pathway. Exp Cell Res. 395(112191)2020.PubMed/NCBI View Article : Google Scholar

8 

Wang X, Shan YQ, Tan QQ, Tan CL, Zhang H, Liu JH, Ke NW, Chen YH and Liu XB: MEX3A knockdown inhibits the development of pancreatic ductal adenocarcinoma. Cancer Cell Int. 20(63)2020.PubMed/NCBI View Article : Google Scholar

9 

Bufalieri F, Caimano M, Lospinoso Severini L, Basili I, Paglia F, Sampirisi L, Loricchio E, Petroni M, Canettieri G, Santoro A, et al: The RNA-binding ubiquitin ligase MEX3A affects glioblastoma tumorigenesis by inducing ubiquitylation and degradation of RIG-I. Cancers (Basel). 12(12)2020.PubMed/NCBI View Article : Google Scholar

10 

Yang D, Jiao Y, Li Y and Fang X: Clinical characteristics and prognostic value of MEX3A mRNA in liver cancer. PeerJ. 8(e8252)2020.PubMed/NCBI View Article : Google Scholar

11 

Jiang H, Zhang X, Luo J, Dong C, Xue J, Wei W, Chen J, Zhou J, Gao Y and Yang C: Knockdown of hMex-3A by small RNA interference suppresses cell proliferation and migration in human gastric cancer cells. Mol Med Rep. 6:575–580. 2012.PubMed/NCBI View Article : Google Scholar

12 

Yang C, Zhan H, Zhao Y, Wu Y, Li L and Wang H: MEX3A contributes to development and progression of glioma through regulating cell proliferation and cell migration and targeting CCL2. Cell Death Dis. 12(14)2021.PubMed/NCBI View Article : Google Scholar

13 

Jin X, Ge LP, Li DQ, Shao ZM, Di GH, Xu XE and Jiang YZ: LncRNA TROJAN promotes proliferation and resistance to CDK4/6 inhibitor via CDK2 transcriptional activation in ER+ breast cancer. Mol Cancer. 19(87)2020.PubMed/NCBI View Article : Google Scholar

14 

Krepischi ACV, Maschietto M, Ferreira EN, Silva AG, Costa SS, da Cunha IW, Barros BDF, Grundy PE, Rosenberg C and Carraro DM: Genomic imbalances pinpoint potential oncogenes and tumor suppressors in Wilms tumors. Mol Cytogenet. 9(20)2016.PubMed/NCBI View Article : Google Scholar

15 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001.PubMed/NCBI View Article : Google Scholar

16 

Pereira B, Sousa S, Barros R, Carreto L, Oliveira P, Oliveira C, Chartier NT, Plateroti M, Rouault JP, Freund JN, et al: CDX2 regulation by the RNA-binding protein MEX3A: Impact on intestinal differentiation and stemness. Nucleic Acids Res. 41:3986–3999. 2013.PubMed/NCBI View Article : Google Scholar

17 

Li H, Liang J, Wang J, Han J, Li S, Huang K and Liu C: Mex3a promotes oncogenesis through the RAP1/MAPK signaling pathway in colorectal cancer and is inhibited by hsa-miR-6887-3p. Cancer Commun (Lond). 41:472–491. 2021.PubMed/NCBI View Article : Google Scholar

18 

Peng W, Li J, Chen R, Gu Q, Yang P, Qian W, Ji D, Wang Q, Zhang Z, Tang J, et al: Upregulated METTL3 promotes metastasis of colorectal Cancer via miR-1246/SPRED2/MAPK signaling pathway. J Exp Clin Cancer Res. 38(393)2019.PubMed/NCBI View Article : Google Scholar

19 

He X, Li S, Yu B, Kuang G, Wu Y, Zhang M, He Y, Ou C and Cao P: LINC00467Up-regulation of promotes the tumourigenesis in colorectal cancer. J Cancer. 10:6405–6413. 2019.PubMed/NCBI View Article : Google Scholar

20 

Wells N, Quigley J, Pascua J, Pinkowski N, Almaiman L, Brasser SM and Hong MY: Effects of low-to-moderate ethanol consumption on colonic growth and gene expression in young adult and middle-aged male rats. PLoS One. 15(e0243499)2020.PubMed/NCBI View Article : Google Scholar

21 

Kumarasamy V, Vail P, Nambiar R, Witkiewicz AK and Knudsen ES: Functional determinants of cell-cycle plasticity and sensitivity to CDK4/6 inhibition. Cancer Res. 81:1347–1360. 2021.PubMed/NCBI View Article : Google Scholar

22 

Shih LJ, Wang JY, Jheng JY, Siao AC, Lin YY, Tsuei YW, Kuo YC, Chuu CP and Kao YH: Betel nut arecoline induces different phases of growth arrest between normal and cancerous prostate cells through the reactive oxygen species pathway. Int J Mol Sci. 21(21)2020.PubMed/NCBI View Article : Google Scholar

23 

Pandey K, Park N, Park KS, Hur J, Cho YB, Kang M, An HJ, Kim S, Hwang S and Moon YW: Combined CDK2 and CDK4/6 inhibition overcomes palbociclib resistance in breast cancer by enhancing senescence. Cancers (Basel). 12(12)2020.PubMed/NCBI View Article : Google Scholar

24 

Qu X, Zhu L, Song L and Liu S: circ_0084927 promotes cervical carcinogenesis by sponging miR-1179 that suppresses CDK2, a cell cycle-related gene. Cancer Cell Int. 20(333)2020.PubMed/NCBI View Article : Google Scholar

25 

Zhao Y, He J, Li Y, Xu M, Peng X, Mao J, Xu B and Cui H: PHF14 promotes cell proliferation and migration through the AKT and ERK1/2 pathways in gastric cancer cells. BioMed Res Int. 2020(6507510)2020.PubMed/NCBI View Article : Google Scholar

26 

Xu X, Tao R, Sun L and Ji X: Exosome-transferred hsa_circ_0014235 promotes DDP chemoresistance and deteriorates the development of non-small cell lung cancer by mediating the miR-520a-5p/CDK4 pathway. Cancer Cell Int. 20(552)2020.PubMed/NCBI View Article : Google Scholar

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Zhou X, Li S, Ma T, Zeng J, Li H, Liu X, Li F, Jiang B, Zhao M, Liu Z, Liu Z, et al: MEX3A knockdown inhibits the tumorigenesis of colorectal cancer via modulating CDK2 expression. Exp Ther Med 22: 1343, 2021.
APA
Zhou, X., Li, S., Ma, T., Zeng, J., Li, H., Liu, X. ... Qin, Y. (2021). MEX3A knockdown inhibits the tumorigenesis of colorectal cancer via modulating CDK2 expression. Experimental and Therapeutic Medicine, 22, 1343. https://doi.org/10.3892/etm.2021.10778
MLA
Zhou, X., Li, S., Ma, T., Zeng, J., Li, H., Liu, X., Li, F., Jiang, B., Zhao, M., Liu, Z., Qin, Y."MEX3A knockdown inhibits the tumorigenesis of colorectal cancer via modulating CDK2 expression". Experimental and Therapeutic Medicine 22.5 (2021): 1343.
Chicago
Zhou, X., Li, S., Ma, T., Zeng, J., Li, H., Liu, X., Li, F., Jiang, B., Zhao, M., Liu, Z., Qin, Y."MEX3A knockdown inhibits the tumorigenesis of colorectal cancer via modulating CDK2 expression". Experimental and Therapeutic Medicine 22, no. 5 (2021): 1343. https://doi.org/10.3892/etm.2021.10778
Copy and paste a formatted citation
x
Spandidos Publications style
Zhou X, Li S, Ma T, Zeng J, Li H, Liu X, Li F, Jiang B, Zhao M, Liu Z, Liu Z, et al: MEX3A knockdown inhibits the tumorigenesis of colorectal cancer via modulating CDK2 expression. Exp Ther Med 22: 1343, 2021.
APA
Zhou, X., Li, S., Ma, T., Zeng, J., Li, H., Liu, X. ... Qin, Y. (2021). MEX3A knockdown inhibits the tumorigenesis of colorectal cancer via modulating CDK2 expression. Experimental and Therapeutic Medicine, 22, 1343. https://doi.org/10.3892/etm.2021.10778
MLA
Zhou, X., Li, S., Ma, T., Zeng, J., Li, H., Liu, X., Li, F., Jiang, B., Zhao, M., Liu, Z., Qin, Y."MEX3A knockdown inhibits the tumorigenesis of colorectal cancer via modulating CDK2 expression". Experimental and Therapeutic Medicine 22.5 (2021): 1343.
Chicago
Zhou, X., Li, S., Ma, T., Zeng, J., Li, H., Liu, X., Li, F., Jiang, B., Zhao, M., Liu, Z., Qin, Y."MEX3A knockdown inhibits the tumorigenesis of colorectal cancer via modulating CDK2 expression". Experimental and Therapeutic Medicine 22, no. 5 (2021): 1343. https://doi.org/10.3892/etm.2021.10778
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team