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Fibroblast growth factor 2 suppresses the expression of C-C motif chemokine 11 through the c-Jun N-terminal kinase pathway in human dental pulp-derived mesenchymal stem cells

  • Authors:
    • Rika Kurogoushi
    • Tomokazu Hasegawa
    • Yuki Akazawa
    • Kokoro Iwata
    • Asuna Sugimoto
    • Kimiko Yamaguchi-Ueda
    • Aya Miyazaki
    • Anrizandy Narwidina
    • Keita Kawarabayashi
    • Takamasa Kitamura
    • Hiroshi Nakagawa
    • Tomonori Iwasaki
    • Tsutomu Iwamoto
  • View Affiliations / Copyright

    Affiliations: Department of Pediatric Dentistry, Institute of Biomedical Sciences, Tokushima University Graduate School, Kuramoto, Tokushima 770-8504, Japan, Department of Pediatric Dentistry/Special Needs Dentistry, Division of Oral Health Sciences, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, Tokyo 113-8549, Japan
    Copyright: © Kurogoushi et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 1356
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    Published online on: September 24, 2021
       https://doi.org/10.3892/etm.2021.10791
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Abstract

The regulation of the mesenchymal stem cell (MSC) programming mechanism promises great success in regenerative medicine. Tissue regeneration has been associated not only with the differentiation of MSCs, but also with the microenvironment of the stem cell niche that involves various cytokines and immune cells in the tissue regeneration site. In the present study, fibroblast growth factor 2 (FGF2), the principal growth factor for tooth development, dental pulp homeostasis and dentin repair, was reported to affect the expression of cytokines in human dental pulp‑derived MSCs. FGF2 significantly inhibited the expression of chemokine C‑C motif ligand 11 (CCL11) in a time‑ and dose‑dependent manner in the SDP11 human dental pulp‑derived MSC line. This inhibition was diminished following treatment with the AZD4547 FGF receptor (FGFR) inhibitor, indicating that FGF2 negatively regulated the expression of CCL11 in SDP11 cells. Furthermore, FGF2 activated the phosphorylation of p38 mitogen‑activated protein kinase (p38 MAPK), extracellular signal‑regulated kinase 1/2 (ERK1/2) and c‑Jun N‑terminal kinases (JNK) in SDP11 cells. The mechanism of the FGFR‑downstream signaling pathway was then studied using the SB203580, U0126 and SP600125 inhibitors for p38 MAPK, ERK1/2, and JNK, respectively. Interestingly, only treatment with SP600125 blocked the FGF2‑mediated suppression of CCL11. The present results suggested that FGF2 regulated the expression of cytokines and suppressed the expression of CCL11 via the JNK signaling pathway in human dental pulp‑derived MSCs. The present findings could provide important insights into the association of FGF2 and CCL11 in dental tissue regeneration therapy.
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Copy and paste a formatted citation
Spandidos Publications style
Kurogoushi R, Hasegawa T, Akazawa Y, Iwata K, Sugimoto A, Yamaguchi-Ueda K, Miyazaki A, Narwidina A, Kawarabayashi K, Kitamura T, Kitamura T, et al: Fibroblast growth factor 2 suppresses the expression of C-C motif chemokine 11 through the c-Jun N-terminal kinase pathway in human dental pulp-derived mesenchymal stem cells. Exp Ther Med 22: 1356, 2021.
APA
Kurogoushi, R., Hasegawa, T., Akazawa, Y., Iwata, K., Sugimoto, A., Yamaguchi-Ueda, K. ... Iwamoto, T. (2021). Fibroblast growth factor 2 suppresses the expression of C-C motif chemokine 11 through the c-Jun N-terminal kinase pathway in human dental pulp-derived mesenchymal stem cells. Experimental and Therapeutic Medicine, 22, 1356. https://doi.org/10.3892/etm.2021.10791
MLA
Kurogoushi, R., Hasegawa, T., Akazawa, Y., Iwata, K., Sugimoto, A., Yamaguchi-Ueda, K., Miyazaki, A., Narwidina, A., Kawarabayashi, K., Kitamura, T., Nakagawa, H., Iwasaki, T., Iwamoto, T."Fibroblast growth factor 2 suppresses the expression of C-C motif chemokine 11 through the c-Jun N-terminal kinase pathway in human dental pulp-derived mesenchymal stem cells". Experimental and Therapeutic Medicine 22.6 (2021): 1356.
Chicago
Kurogoushi, R., Hasegawa, T., Akazawa, Y., Iwata, K., Sugimoto, A., Yamaguchi-Ueda, K., Miyazaki, A., Narwidina, A., Kawarabayashi, K., Kitamura, T., Nakagawa, H., Iwasaki, T., Iwamoto, T."Fibroblast growth factor 2 suppresses the expression of C-C motif chemokine 11 through the c-Jun N-terminal kinase pathway in human dental pulp-derived mesenchymal stem cells". Experimental and Therapeutic Medicine 22, no. 6 (2021): 1356. https://doi.org/10.3892/etm.2021.10791
Copy and paste a formatted citation
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Spandidos Publications style
Kurogoushi R, Hasegawa T, Akazawa Y, Iwata K, Sugimoto A, Yamaguchi-Ueda K, Miyazaki A, Narwidina A, Kawarabayashi K, Kitamura T, Kitamura T, et al: Fibroblast growth factor 2 suppresses the expression of C-C motif chemokine 11 through the c-Jun N-terminal kinase pathway in human dental pulp-derived mesenchymal stem cells. Exp Ther Med 22: 1356, 2021.
APA
Kurogoushi, R., Hasegawa, T., Akazawa, Y., Iwata, K., Sugimoto, A., Yamaguchi-Ueda, K. ... Iwamoto, T. (2021). Fibroblast growth factor 2 suppresses the expression of C-C motif chemokine 11 through the c-Jun N-terminal kinase pathway in human dental pulp-derived mesenchymal stem cells. Experimental and Therapeutic Medicine, 22, 1356. https://doi.org/10.3892/etm.2021.10791
MLA
Kurogoushi, R., Hasegawa, T., Akazawa, Y., Iwata, K., Sugimoto, A., Yamaguchi-Ueda, K., Miyazaki, A., Narwidina, A., Kawarabayashi, K., Kitamura, T., Nakagawa, H., Iwasaki, T., Iwamoto, T."Fibroblast growth factor 2 suppresses the expression of C-C motif chemokine 11 through the c-Jun N-terminal kinase pathway in human dental pulp-derived mesenchymal stem cells". Experimental and Therapeutic Medicine 22.6 (2021): 1356.
Chicago
Kurogoushi, R., Hasegawa, T., Akazawa, Y., Iwata, K., Sugimoto, A., Yamaguchi-Ueda, K., Miyazaki, A., Narwidina, A., Kawarabayashi, K., Kitamura, T., Nakagawa, H., Iwasaki, T., Iwamoto, T."Fibroblast growth factor 2 suppresses the expression of C-C motif chemokine 11 through the c-Jun N-terminal kinase pathway in human dental pulp-derived mesenchymal stem cells". Experimental and Therapeutic Medicine 22, no. 6 (2021): 1356. https://doi.org/10.3892/etm.2021.10791
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