MicroRNA‑361 reduces the viability and migratory ability of pancreatic cancer cells via mediation of the MAPK/JNK pathway

Wang,Juan; Xie,Zongjing; Liu,Yan; Zhang,Weiguo; Ji,Tingting

doi:10.3892/etm.2021.10799

MicroRNA‑361 reduces the viability and migratory ability of pancreatic cancer cells via mediation of the MAPK/JNK pathway

Authors:
- Juan Wang
- Zongjing Xie
- Yan Liu
- Weiguo Zhang
- Tingting Ji
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Affiliations: Department of Gastroenterology, People's Hospital of Leling City, Dezhou, Shandong 253600, P.R. China, Department of General Surgery, Zhucheng People's Hospital, Weifang, Shandong 262200, P.R. China, Department of Gastroenterology, Qiqihar Jianhua Hospital, Qiqihar, Heilongjiang 161000, P.R. China, Second Department of General Surgery, Tianjin Fifth Central Hospital, Tianjin 300450, P.R. China, Department of Gastroenterology, No. 215 Hospital of Shaanxi Nuclear Industry, Xianyang, Shaanxi 712000, P.R. China
Published online on: September 27, 2021 https://doi.org/10.3892/etm.2021.10799
Article Number: 1365
Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Previous research has revealed that microRNA‑361 (miR‑361) functions as a fundamental modulator in non‑small‑cell lung cancer and esophageal carcinoma. However, its involvement in pancreatic cancer (PC) is yet to be elucidated. Therefore, the present study aimed to examine the mechanism and function of miR‑361 during the regulation of PC cell migration and viability. It was demonstrated that miR‑361 expression decreased in PC cell lines and tissues, and the overexpression of miR‑361 suppressed in vivo PC cell proliferation in mice. Moreover, flow cytometry and MTT assays indicated that the miR‑361 mimic decreased the viability and increased the apoptosis of PC cells. Both Transwell migration and wound healing assays identified that miR‑361 ameliorated the migratory ability of PC cells. Using dual‑luciferase reporter assays, it was found that miR‑361 targeted mitogen‑activated protein kinase (MAPK)/JNK 3'‑untranslated regions, inducing the downregulation of this gene. In PC cells, overexpression of MAPK/JNK diminished the pro‑apoptotic effect of the miR‑361 mimic, while restoring the migratory activity of PC cells. Collectively, the present results suggested novel molecular mechanisms underlying PC progression and development.

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